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Abasic CRISPR RNAs inherently harness fidelity of SpCas9 for genome editing

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CRISPR-Cas9, an RNA-guided immune system, functions specifically in bacteria while controlling autoimmunity. However, its application to genome editing often causes deleterious off-target cleavages. Here, by sequencing CRISPR RNAs (crRNAs), we discovered abasi... ...