A telescopic microscope equipped with a quanta image sensor for live-cell bioluminescence imaging [0.03%]
一种用于活细胞生物发光成像的量子图像传感器显微镜
Ruyu Ma,Luciano M Santino,Tomáš Chobola et al.
Ruyu Ma et al.
Bioluminescence is an attractive alternative to fluorescence for live-cell imaging; however, its low intensity has prevented widespread adoption. Specialized microscopes compensate by sacrificing spatial resolution, field of view and dynami...
Decoding post-transcriptional regulatory networks by RNA-linked CRISPR screening in human cells [0.03%]
通过人类细胞中与RNA相连的CRISPR筛选解码转录后调控网络
Patrick J Nugent,Heungwon Park,Cynthia L Wladyka et al.
Patrick J Nugent et al.
RNAs undergo a complex choreography of metabolic processes that are regulated by thousands of RNA-associated proteins. Here we introduce ReLiC, a scalable and high-throughput RNA-linked CRISPR approach to measure the responses of diverse RN...
SAVANA: reliable analysis of somatic structural variants and copy number aberrations using long-read sequencing [0.03%]
基于长读测序可靠分析体细胞结构变异和拷贝数异常的SAVANA方法
Hillary Elrick,Carolin M Sauer,Jose Espejo Valle-Inclan et al.
Hillary Elrick et al.
Accurate detection of somatic structural variants (SVs) and somatic copy number aberrations (SCNAs) is critical to study the mutational processes underpinning cancer evolution. Here we describe SAVANA, an algorithm designed to detect somati...
InterpolAI: deep learning-based optical flow interpolation and restoration of biomedical images for improved 3D tissue mapping [0.03%]
基于深度学习的生物医学图像光流插值及复原以提升三维组织映射效果
Saurabh Joshi,André Forjaz,Kyu Sang Han et al.
Saurabh Joshi et al.
Recent advances in imaging and computation have enabled analysis of large three-dimensional (3D) biological datasets, revealing spatial composition, morphology, cellular interactions and rare events. However, the accuracy of these analyses ...
Vivien Marx
Vivien Marx
Single-cell ultra-high-throughput multiplexed chromatin and RNA profiling reveals gene regulatory dynamics [0.03%]
单细胞超高通量染色质和RNA多重分析揭示基因调控动力学
Sara Lobato-Moreno,Umut Yildiz,Annique Claringbould et al.
Sara Lobato-Moreno et al.
Enhancers and transcription factors (TFs) are crucial in regulating cellular processes. Current multiomic technologies to study these elements in gene regulatory mechanisms lack multiplexing capability and scalability. Here we present singl...
Laura C Kida,Caleb A Lareau
Laura C Kida
Daniel C Jones,Anna E Elz,Azadeh Hadadianpour et al.
Daniel C Jones et al.
Single-cell spatial transcriptomics promises a highly detailed view of a cell's transcriptional state and microenvironment, yet inaccurate cell segmentation can render these data murky by misattributing large numbers of transcripts to nearb...