A highly photostable monomeric red fluorescent protein for dual-color 3D STED and time-lapse 3D SIM imaging [0.03%]
一种高光稳定的单体红色荧光蛋白，用于双色3D受激发射损耗和时间推移3D结构照明成像
Ya Ding,Wenting He,Kunhao Wang et al.
Ya Ding et al.
Highly photostable red fluorescent proteins (RFPs) are invaluable for dual-color fluorescence microscopy, including super-resolution microscopy. Here we present mScarlet3‑S2, an RFP that exhibits a 29-fold improvement in photostability ove...
From slices to deep dishes: spatial transcriptomics and translatomics of thick tissue blocks [0.03%]
从切片到深盘：厚组织块的空间转录组和翻译组
Maroš Pleška,Sanja Vickovic
Maroš Pleška
Helixer: ab initio prediction of primary eukaryotic gene models combining deep learning and a hidden Markov model [0.03%]
Helixer：结合深度学习和隐马尔可夫模型从头预测真核生物基因模型
Felix Holst,Anthony M Bolger,Felicitas Kindel et al.
Felix Holst et al.
The accurate identification of genes is vital for understanding biological function, yet this remains challenging across many newly sequenced or less-studied species. Here we present Helixer, an artificial intelligence-based tool for ab ini...
Scalable spatial single-cell transcriptomics and translatomics in 3D thick tissue blocks [0.03%]
厚组织块中可扩展的空间单细胞转录组和翻译组学
Xin Sui,Jennifer A Lo,Shuchen Luo et al.
Xin Sui et al.
Characterizing the transcriptional and translational gene expression patterns at the single-cell level within their three-dimensional (3D) tissue context is essential for revealing how genes shape tissue structure and function in health and...
TIRTL-seq: deep, quantitative and affordable paired TCR repertoire sequencing [0.03%]
TIRTL-seq：深入、定量且低成本的配对TCR库测序
Mikhail V Pogorelyy,Allison M Kirk,Samir Adhikari et al.
Mikhail V Pogorelyy et al.
The specificity of T cells is determined by T cell receptor (TCR) α and β chain sequences. While bulk TCR sequencing enables cost-effective repertoire profiling without chain pairing information, single-cell approaches provide paired data...
Zijian Niu,Thibault Bruyère,David Manthey et al.
Zijian Niu et al.
4Pi-SIMFLUX: 4Pi single-molecule localization microscopy with structured illumination [0.03%]
4Pi-SIMFLUX：具有结构照明的4Pi单分子定位显微镜
Qian Wang,Bei Zheng,Zijing Yu et al.
Qian Wang et al.
Single-molecule localization microscopy (SMLM) has transformed biological imaging by enabling nanoscale visualization of intricate subcellular structures. However, conventional three-dimensional SMLM techniques typically exhibit lower axial...
Author Correction: Confocal Airy beam oblique light-sheet tomography for brain-wide cell type distribution and morphology [0.03%]
作者更正：用于大脑范围细胞类型分布和形态的共聚焦艾里束斜光片层析术
Xiaoli Qi,Rodrigo Muñoz-Castañeda,Yuanlei Yue et al.
Xiaoli Qi et al.
Published Erratum
Nature methods. 2025 Nov 19. DOI:10.1038/s41592-025-02982-y 2025