eSIG-Net: an interaction language model that decodes the protein code of single mutations [0.03%]
基于单点突变解码蛋白质编码的交互语言模型
Xingxin Pan,Aditya Shrawat,Sidharth Raghavan et al.
Xingxin Pan et al.
Most proteins act through interactions with other molecules, yet predicting how single mutations perturb these interactions-defined as 'protein codes'-remains a central challenge in computational biology. Here we introduce eSIG-Net, the edg...
Single-molecule localization and diffusivity microscopy reveals dynamic biomolecular organization in living cells [0.03%]
单分子定位及扩散显微镜揭示活细胞中生物大分子的动态组织规律
Zuhui Wang,Yiwen Liu,Bo Wang et al.
Zuhui Wang et al.
Single-molecule tracking in living cells measures protein diffusivity but requires sparse imaging, limiting high-density mapping. Here we introduce single-molecule localization and diffusivity microscopy (SMLDM), a deep learning-based appro...
Honghao Cao,Sarah Spitz,Li-Yu Yu et al.
Honghao Cao et al.
The formation of organized optical states in multidimensional systems is crucial for understanding light-matter interaction and advancing light-shaping technologies. Here we report the observation of a self-localized, ultrafast pencil beam ...
Spatial 5mC-seq profiling of embryos and decidua after implantation in mammals [0.03%]
哺乳动物着床后胚胎和蜕膜的全基因组5mC- seq图谱分析
Xun Shan,Yimin Tang,Jinzhou Hu et al.
Xun Shan et al.
DNA methylation is vital for development and diseases, yet no spatial DNA methylation profiling technology has been reported. Here we developed spatial 5mC-seq (SmC-seq), a microfluidic-based method providing unbiased genome-wide methylome ...
Systematically decoding pathological morphologies and molecular profiles with unified multimodal embedding [0.03%]
统一多模态嵌入病理形态和分子特征的系统解码方法
Peng Zhang,Chaofei Gao,Kui Hua et al.
Peng Zhang et al.
Systematic cross-modality inference and integration of pathological morphologies and multilayer molecular profiles have advanced disease biology; however, methodological challenges remain in multimodal learning. Here, we present Multi-Embed...
PinkyCaMP: an mScarlet-based calcium sensor with enhanced brightness, photostability and multiplexing capabilities [0.03%]
PinkyCaMP：一种基于mScarlet的钙离子传感器，具有增强的亮度、光稳定性和多重检测能力
Ryan Fink,Shosei Imai,Nala Gockel et al.
Ryan Fink et al.
Genetically encoded calcium (Ca2+) indicators (GECIs) are essential tools for monitoring neuronal activity, but the performance of red fluorescent GECIs has remained limited. In particular, many red indicators are relatively dim, produce lo...
Direct RNA sequencing and signal alignment reveal RNA structure ensembles in a eukaryotic cell [0.03%]
直接RNA测序和信号比对揭示了真核细胞中的RNA结构组合体
Jiaxu Wang,Jian Han,Wen Ting Tan et al.
Jiaxu Wang et al.
The extent to which an RNA folds into structure ensembles and how different structures in the ensemble regulate eukaryotic gene expression is not fully understood. Here, we coupled chemical probing with direct RNA sequencing to identify str...
Agustin A Corbat,Christa G Walther,Laura R de la Ballina et al.
Agustin A Corbat et al.
Synthetic multicolor antigen-stabilizable nanobody platform for intersectional labeling and functional imaging [0.03%]
合成多色抗原稳定化纳米体平台用于交联标记和功能成像
Natalia V Barykina,Erin M Carey,Olena S Oliinyk et al.
Natalia V Barykina et al.
We present a synthetic tool kit of antigen-stabilizable fluorescent nanobodies (VIS-Fbs) spanning the entire visible spectrum from 450 nm to 660 nm. By engineering over 20 fluorescent proteins (FPs) and biosensors into 8 nanobodies, we esta...