Neuropixels Opto: combining high-resolution electrophysiology and optogenetics [0.03%]
神经像素光遗传学：高分辨率电生理与光遗传学的结合
Anna A Lakunina,Karolina Z Socha,Alexander E Ladd et al.
Anna A Lakunina et al.
High-resolution extracellular electrophysiology is the gold standard for recording spikes from distributed neural populations and is especially powerful when combined with optogenetics for manipulation of specific cell types with high tempo...
Vivien Marx
Vivien Marx
TADShop: systematic benchmarking and identification of topologically associating domains [0.03%]
TADShop：拓扑相关域的系统基准测试与识别
Pumin Li,Andras Hatos,Miljan Petrovic et al.
Pumin Li et al.
Topologically associating domains (TADs) are structural units of chromatin organization. Their definition and identification rely on computational analyses of chromosome conformation capture data. Here we systematically assessed and compare...
External validation improves generalizability, replicability and reproducibility in predictive models for neuroimaging [0.03%]
外部验证可提高神经影像预测模型的普遍性、可重复性和再现性
Matthew Rosenblatt,Maya L Foster,Brendan D Adkinson et al.
Matthew Rosenblatt et al.
Poor generalizability continues to hinder mapping brain activity to behavior using human neuroimaging data. A potential solution is predictive modeling, which evaluates generalizability in unseen data from the same dataset. However, predict...
Keren Zhou,Junhong Huang,Shurong Liu et al.
Keren Zhou et al.
RNA molecules interact extensively with each other and with RNA-binding proteins (RBPs) to regulate gene expression. To improve detection of RBP-RNA and RNA-RNA interactions, we developed the rbsSeeker and rriScan tools and integrated the i...
AreTomoLive: automated reconstruction of comprehensively corrected and denoised cryo-electron tomograms in real time and at high throughput [0.03%]
实时高通量重建全面校正和降噪的冷冻电子断层扫描图像的自动化系统（AreTomoLive）
Ariana Peck,Yue Yu,Mohammadreza Paraan et al.
Ariana Peck et al.
High-throughput data processing is necessary to realize the full potential of cryo-electron tomography and subtomogram averaging. The field's fragmented software landscape remains a considerable hurdle to this end. Here we present AreTomoLi...
Stefan Oberlin,Neil Q Tay,Albert Xue et al.
Stefan Oberlin et al.
CRISPR-based genetic perturbation screens have revolutionized the ability to link genes to cellular phenotypes with unprecedented precision and scale; however, conventional pooled CRISPR screens require large cell numbers to achieve adequat...
Decoding sequence determinants of gene expression in diverse cellular and disease states [0.03%]
解析多种细胞和疾病状态下基因表达的序列决定因素
Avantika Lal,Alexander Karollus,Laura Gunsalus et al.
Avantika Lal et al.
Sequence-to-function models that predict gene expression from genomic DNA sequence have proven valuable for many biological tasks, including understanding cis-regulatory syntax and interpreting noncoding genetic variation. However, current ...
Johannes Linder,David R Kelley
Johannes Linder
Subcellular chemical mapping using correlated cryogenic electron and mass spectrometry imaging [0.03%]
利用相关低温电子和质谱成像进行亚细胞化学图谱绘制
Hannah Ochner,Buse Isbilir,Sonja Blasche et al.
Hannah Ochner et al.
Electron cryomicroscopy (cryo-EM) allows high spatial resolution visualization of biological specimens; however, it is challenging to chemically identify densities observed in cryo-EM. To overcome this, we combined cryo-EM with chemical ima...