The Cousa objective: a long-working distance air objective for multiphoton imaging in vivo [0.03%]
Cousa物镜：一种用于体内多光子成像的长工作距离空气物镜
Che-Hang Yu,Yiyi Yu,Liam M Adsit et al.
Che-Hang Yu et al.
Multiphoton microscopy can resolve fluorescent structures and dynamics deep in scattering tissue and has transformed neural imaging, but applying this technique in vivo can be limited by the mechanical and optical constraints of conventiona...
Andrea Bonini,Adina Sauciuc,Giovanni Maglia
Andrea Bonini
Oda Helene Schiøtz,Christoph J O Kaiser,Sven Klumpe et al.
Oda Helene Schiøtz et al.
Cryo-focused ion beam milling of frozen-hydrated cells and subsequent cryo-electron tomography (cryo-ET) has enabled the structural elucidation of macromolecular complexes directly inside cells. Application of the technique to multicellular...
A data analysis framework for combining multiple batches increases the power of isobaric proteomics experiments [0.03%]
一种数据分析框架：结合多个批次的数据以增强等压蛋白质组学实验的功效
Jonathon J O&#x;Brien,Anil Raj,Aleksandr Gaun et al.
Jonathon J O&#x;Brien et al.
We present a framework for the analysis of multiplexed mass spectrometry proteomics data that reduces estimation error when combining multiple isobaric batches. Variations in the number and quality of observations have long complicated the ...
Sriram Subramaniam
Sriram Subramaniam
DeepMainmast: integrated protocol of protein structure modeling for cryo-EM with deep learning and structure prediction [0.03%]
基于深度学习和结构预测的冷冻电镜蛋白质结构建模集成协议
Genki Terashi,Xiao Wang,Devashish Prasad et al.
Genki Terashi et al.
Three-dimensional structure modeling from maps is an indispensable step for studying proteins and their complexes with cryogenic electron microscopy. Although the resolution of determined cryogenic electron microscopy maps has generally imp...
TubULAR: tracking in toto deformations of dynamic tissues via constrained maps [0.03%]
基于约束映射的动态组织整体变形跟踪方法(TUBULAR)
Noah P Mitchell,Dillon J Cislo
Noah P Mitchell
A common motif in biology is the arrangement of cells into tubes, which further transform into complex shapes. Traditionally, analysis of dynamic tissues has relied on inspecting static snapshots, live imaging of cross-sections or tracking ...
François Graner
François Graner
Embryo mechanics cartography: inference of 3D force atlases from fluorescence microscopy [0.03%]
胚胎力学图谱：从荧光显微镜图像推断三维力图集
Sacha Ichbiah,Fabrice Delbary,Alex McDougall et al.
Sacha Ichbiah et al.
Tissue morphogenesis results from a tight interplay between gene expression, biochemical signaling and mechanics. Although sequencing methods allow the generation of cell-resolved spatiotemporal maps of gene expression, creating similar map...