Yu Xiao,Zhiliang Bai,Zhuoning Zou et al.
Yu Xiao et al.
N6-methyladenosine (m6A) on RNA plays diverse regulatory roles, yet its spatial distribution within tissues remains largely unexplored. Here we introduce m6A-ARTR-DBiT, a spatial m6A profiling assay that leverages reverse-transcription-base...
OrthoFinder: improved phylogenetic orthology inference with enhanced accuracy and scalability [0.03%]
OrthoFinder：改进的系统发育直系同源物推断方法，具有更高的准确性和可扩展性
David M Emms,Yi Liu,Laurence Belcher et al.
David M Emms et al.
Here we present a major advancement of the OrthoFinder method. This extends OrthoFinder's high-accuracy comparative genomic framework to provide substantially enhanced scalability and accuracy. Specifically, we show that enhanced phylogenet...
Simultaneous two- and three-photon multiplane imaging across cortical layers in freely moving mice [0.03%]
自由活动小鼠皮层各层的双光子和三光子多平面成像同时进行
Alexandr Klioutchnikov,Damian J Wallace,Caleb Berdahl et al.
Alexandr Klioutchnikov et al.
Head-mounted multiphoton microscopes enable imaging of activity from neuronal populations spread throughout the cortical layers in freely moving mice but so far have been restricted to recording from one cortical layer at a time. Here, comb...
Evaluating the role of pretraining dataset size and diversity on single-cell foundation model performance [0.03%]
评估预训练数据集大小和多样性对单细胞基础模型性能的作用
Alan DenAdel,Madeline Hughes,Akshaya Thoutam et al.
Alan DenAdel et al.
The success of transformer-based foundation models on natural language and images has motivated their use in single-cell biology. Single-cell foundation models have been trained on increasingly larger transcriptomic datasets, scaling from i...
A scalable approach to investigating sequence-to-function predictions from personal genomes [0.03%]
一种探究个人基因组序列与功能预测的可扩展方法
Anna E Spiro,Xinming Tu,Yilun Sheng et al.
Anna E Spiro et al.
Sequence-to-function (S2F) models can evaluate arbitrary DNA sequences, yet they struggle to fully capture inter-individual variation in gene expression. We introduce SAGE-net, a scalable framework for training and evaluating S2F models usi...
Chromatix: a differentiable, GPU-accelerated wave-optics library [0.03%]
Chromatix：一种可微分的、基于GPU的波动光学库
Diptodip Deb,Gert-Jan Both,Eric Bezzam et al.
Diptodip Deb et al.
Many current microscopy methods incorporate computational modeling as an integral part of the imaging process, either to solve inverse problems or optimize the optical system design itself. These methods often depend on differentiable optic...
Brightness demixing for simultaneous multi-target imaging in 3D single-molecule localization microscopy [0.03%]
三维单分子定位显微镜中用于同时多目标成像的亮度分离技术
Laurent Le,Surabhi K Sreenivas,Emmanuel Fort et al.
Laurent Le et al.
Single-molecule localization microscopy has enabled high-resolution imaging, but the simultaneous detection of multiple fluorophores traditionally relies on spectral-based separation, which is inherently constrained by spectral overlap. Her...