Translocation of linearized full-length proteins through an engineered nanopore under opposing electrophoretic force [0.03%]
在相反电场力作用下通过工程纳米孔的全长线性化蛋白质的转移
Adina Sauciuc,Blasco Morozzo Della Rocca,Matthijs Jonathan Tadema et al.
Adina Sauciuc et al.
Nanopores have recently been used to identify and fingerprint proteins. However, because proteins, unlike DNA, do not have a uniform charge, the electrophoretic force cannot in general be used to translocate or linearize them. Here we show ...
Melanie Senior
Melanie Senior
Charles Schmidt
Charles Schmidt
Targeted genome editing with a DNA-dependent DNA polymerase and exogenous DNA-containing templates [0.03%]
一种DNA依赖性DNA聚合酶与外源模板协同作用实现靶向基因组编辑
Bin Liu,Xiaolong Dong,Chunwei Zheng et al.
Bin Liu et al.
Reverse transcriptases, used in prime editing systems, exhibit lower fidelity, processivity and dNTP affinity than many DNA-dependent DNA polymerases. We report that a DNA-dependent DNA polymerase (phi29), untethered from Cas9, enables edit...
Anuradha Wickramarachchi,Brendan Hosking,Yatish Jain et al.
Anuradha Wickramarachchi et al.
Hervé Vanderschuren,Patience Chatukuta,Detlef Weigel et al.
Hervé Vanderschuren et al.
Self-organizing models of human trunk organogenesis recapitulate spinal cord and spine co-morphogenesis [0.03%]
自组织的人体躯干器官发生模型再现脊髓和椎骨的协同发生过程
Simona Gribaudo,Rémi Robert,Björn van Sambeek et al.
Simona Gribaudo et al.
Integrated in vitro models of human organogenesis are needed to elucidate the multi-systemic events underlying development and disease. Here we report the generation of human trunk-like structures that model the co-morphogenesis, patterning...
Mengru Lyu,Jiyuan Chen,Yeheng Peng et al.
Mengru Lyu et al.
Cormac Sheridan
Cormac Sheridan