Sensitive and unbiased genome-wide profiling of base-editor-induced off-target activity using CHANGE-seq-BE [0.03%]
利用CHANGE-seq-BE对碱基编辑器诱导的脱靶活性进行敏感和无偏向性的全基因组分析
Cicera R Lazzarotto,Varun Katta,Yichao Li et al.
Cicera R Lazzarotto et al.
Detection of the off-target effects of base editors is important for identifying their safety risks but current methods for understanding their global activities have limitations in terms of sensitivity or bias by computationally selecting ...
Effective combinatorial antifungal therapy using a host defense peptide mimic that self-assembles into delivery micelles [0.03%]
一种宿主防御肽类似物自组装成递送米托尔的有效的组合抗真菌疗法
Longqiang Liu,Min Zhou,Ximian Xiao et al.
Longqiang Liu et al.
The synergistic combination of two antimicrobial drugs is a promising therapeutic modality for many infectious diseases. However, systemic fungal infections still have a high mortality rate because of distinct in vivo distributions of the t...
Single-strand deaminase-assisted editing for functional RNA manipulation [0.03%]
单链去氨酶介导的RNA编辑技术用于功能RNA修饰
Yuan Zhuang,Qingguo Zhu,Hao Wu et al.
Yuan Zhuang et al.
Rewriting RNA information to alter function requires controllable tools to edit RNA sequences within a user-defined region. Here we report a single-strand deaminase-assisted platform for adjustable RNA information manipulation (AIM). AIM is...
Florian Trigodet,Rohan Sachdeva,Jillian F Banfield et al.
Florian Trigodet et al.
Assessing the accuracy of long-read assemblies, especially from complex environmental metagenomes that include underrepresented organisms, is challenging. Here we benchmark four state-of-the-art long-read assembly software programs, HiCanu,...
Publisher Correction: Computational prediction of human genetic variants in the mouse genome [0.03%]
出版更正：计算预测人类基因组变异在小鼠基因组中的位置
Kexin Dong,Samuel I Gould,Minghang Li et al.
Kexin Dong et al.
Published Erratum
Nature biotechnology. 2025 Dec 29. DOI:10.1038/s41587-025-02991-4 2025
Machine learning enables scalable and systematic hierarchical virus taxonomy [0.03%]
机器学习使病毒的系统化层级分类可扩展
Benjamin Bolduc,Olivier Zablocki,Dann Turner et al.
Benjamin Bolduc et al.
Although virus ecogenomics has expanded access to and understanding of the virosphere, existing classification tools lack taxonomic resolution and are unable to scale to modern discovery-based datasets or classify previously unknown sequenc...
The import of biological research material is a silent barrier to biotechnology in the Global South [0.03%]
生物研究材料的进口是发展中国家生物技术的一个隐性障碍
Gabriela Bortz,Lilia Stubrin,Rafael Anta
Gabriela Bortz
Engineered base editors with reduced bystander editing through directed evolution [0.03%]
通过定向进化降低脱靶编辑的工程化碱基编辑器
Ramiro M Perrotta,Svenja Vinke,Raphaël Ferreira et al.
Ramiro M Perrotta et al.
Base editors enable precise genome modification but are constrained by bystander edits that limit their applicability. Existing strategies to enhance precision often compromise efficiency and remain highly sequence dependent. Here we presen...
Computational prediction of human genetic variants in the mouse genome [0.03%]
人类遗传变异的计算机预测在小鼠基因组中的应用
Kexin Dong,Samuel I Gould,Minghang Li et al.
Kexin Dong et al.
The design of genetically engineered mouse models would benefit from a computational pipeline to predict mouse genetic variants that mirror the sequence and functional effects of human disease variants. Here we present H2M (human-to-mouse),...