Jesse D Buffington,Hung-Che Kuo,Kuang Hu et al.
Jesse D Buffington et al.
Retrons can produce multicopy single-stranded DNA in cells through self-primed reverse transcription. However, their potential for inserting genetic cargos in eukaryotes remains largely unexplored. Here we report the discovery and engineeri...
Live imaging of late-stage preimplantation human embryos reveals de novo mitotic errors [0.03%]
活体成像揭示晚期植入前人类胚胎出现新的有丝分裂错误
Ahmed Abdelbaki,Afshan McCarthy,Anita Karsa et al.
Ahmed Abdelbaki et al.
Existing methods to image chromosome segregation errors are not suitable for studying human embryos at advanced preimplantation stages. As chromosomal errors are a leading cause of miscarriage and infertility, it remains unclear whether mis...
Corentin Mollier,Jean-Léon Maître
Corentin Mollier
Overcoming barriers to the wide adoption of single-cell large language models in biomedical research [0.03%]
克服单细胞大型语言模型在生物医学研究中广泛应用的障碍
Fang Xie,Bingkang Zhao,Songxiang Xu et al.
Fang Xie et al.
Aidan C Daly,Francesco Cambuli,Tarmo Äijö et al.
Aidan C Daly et al.
Tissue structure and molecular circuitry in the colon can be profoundly impacted by systemic age-related effects but many of the underlying molecular cues remain unclear. Here, we build a cellular and spatial atlas of the colon across three...
Laine Goudy,Alvin Ha,Ashir A Borah et al.
Laine Goudy et al.
Targeted epigenetic engineering of gene expression in cell therapies would allow programming of desirable phenotypes without many of the challenges and safety risks associated with double-strand break-based genetic editing approaches. Here,...
AlphaDIA enables DIA transfer learning for feature-free proteomics [0.03%]
基于特征的蛋白质组学的AlphaDIA使DIA迁移学习成为可能
Georg Wallmann,Patricia Skowronek,Vincenth Brennsteiner et al.
Georg Wallmann et al.
The scale of data generated for mass-spectrometry-based proteomics and modern acquisition strategies poses a challenge to bioinformatic analysis. Search engines need to make optimal use of the data for biological discoveries while remaining...
Charles Schmidt
Charles Schmidt
A tumor-on-a-chip for in vitro study of CAR-T cell immunotherapy in solid tumors [0.03%]
一种用于体外研究CAR-T细胞免疫疗法在实体瘤中的作用的肿瘤芯片
Haijiao Liu,Estela Noguera-Ortega,Xuanqi Dong et al.
Haijiao Liu et al.
Our limited understanding of cancer-immune interactions remains a critical barrier to advancing chimeric antigen receptor (CAR)-T cell therapy for solid malignancies. Here, we present a microengineered system that enables vascularization of...
Gordana Vunjak-Novakovic
Gordana Vunjak-Novakovic