CRISPR live-cell imaging reveals chromatin dynamics and enhancer interactions at multiple non-repetitive loci [0.03%]
CRISPR活细胞成像揭示多处非重复位点的染色质动态和增强子相互作用
Meishuo Liu,Keyun Huang,Jie Zhang et al.
Meishuo Liu et al.
Existing methods to visualize dynamic changes in the three-dimensional genome, promoter-enhancer interactions and the influence of epigenetic modifications in non-repetitive loci are limited. Here we introduce CRISPR PRO-LiveFISH (Pooled gR...
Andrew Marshall
Andrew Marshall
Malte Kuehl,Darius P Schaub,Francesco Carli et al.
Malte Kuehl et al.
A nonsurgical brain implant enabled through a cell-electronics hybrid for focal neuromodulation [0.03%]
基于细胞-电子混合技术的非手术脑植入设备实现焦点神经调控
Shubham Yadav,Ray X Lee,Shivam N Kajale et al.
Shubham Yadav et al.
Bioelectronic implants for brain stimulation are used to treat brain disorders but require invasive surgery. To provide a noninvasive alternative, we report nonsurgical implants consisting of immune cell-electronics hybrids, an approach we ...
KATMAP infers splicing factor activity and regulatory targets from knockdown data [0.03%]
基于敲除数据推断剪接因子活性和调控靶点的KATMAP方法
Michael P McGurk,David C McWatters,Christopher B Burge
Michael P McGurk
Typical RNA sequencing (RNA-seq) experiments uncover hundreds of splicing changes, reflecting underlying changes in splicing factor (SF) activity. Understanding how SF activity influences transcriptomic variation requires elucidating how ea...
Author Correction: Simultaneous editing of three homoeoalleles in hexaploid bread wheat confers heritable resistance to powdery mildew [0.03%]
作者更正：同时编辑六倍体小麦中的三个同源等位基因可赋予对该病具有遗传抗性的小麦品种
Yanpeng Wang,Xi Cheng,Qiwei Shan et al.
Yanpeng Wang et al.
Published Erratum
Nature biotechnology. 2025 Nov 3. DOI:10.1038/s41587-025-02916-1 2025
Growth-coupled microbial biosynthesis of the animal pigment xanthommatin [0.03%]
与生长耦合的微生物生物合成动物色素_xanthommatin
Leah B Bushin,Tobias B Alter,María V G Alván-Vargas et al.
Leah B Bushin et al.
Engineering heterologous natural product pathways in bacteria has achieved broad success but most approaches suffer from low initial production levels that require extensive, resource-heavy iterative strain optimization. Xanthommatin is a s...
Josquin Moraly,Jaehyun Suh,Naomi Taylor
Josquin Moraly
Mesenchymal thymic niche cells enable regeneration of the adult thymus and T cell immunity [0.03%]
间充质胸腺生态位细胞能够使成人胸腺和T细胞免疫再生
Karin Gustafsson,Sergey Isaev,Kamdin Mirsanaye et al.
Karin Gustafsson et al.
Thymic atrophy and the progressive immune decline that accompanies it is a major health problem, chronically with age and acutely with immune injury. No definitive solution is available. Here we demonstrate that one of the three mesenchymal...
High-plex spatial RNA imaging in one round with conventional microscopes using color-intensity barcodes [0.03%]
使用颜色强度条形码在一轮中用常规显微镜进行高通量空间RNA成像
Tianyi Chang,Shihui Zhao,Kunyue Deng et al.
Tianyi Chang et al.
Spatial RNA imaging has not been widely adopted because conventional fluorescence microscopy is limited to only a few channels and the cyclic reactions needed to increase multiplexing in techniques such as sequential fluorescence in situ hy...