Dario R Alessi,Suzanne R Pfeffer
Dario R Alessi
Activating mutations in leucine-rich repeat kinase 2 (LRRK2) represent the most common cause of monogenic Parkinson's disease. LRRK2 is a large multidomain protein kinase that phosphorylates a specific subset of the ∼65 human Rab GTPases, ...
Harald W Platta,Julia Jeske,Nadine Schmidt et al.
Harald W Platta et al.
Peroxisomes are organelles that play a central role in lipid metabolism and cellular redox homeostasis. The import of peroxisomal matrix proteins by peroxisomal targeting signal (PTS) receptors is an ATP-dependent mechanism. However, the en...
The Bis(monoacylglycero)-phosphate Hypothesis: From Lysosomal Function to Therapeutic Avenues [0.03%]
双单酰基甘油磷酸假说：从溶酶体功能到治疗途径
Uche N Medoh,Monther Abu-Remaileh
Uche N Medoh
Lysosomes catabolize and recycle lipids and other biological molecules to maintain cellular homeostasis in diverse nutrient environments. Lysosomal lipid catabolism relies on the stimulatory activity of bis(monoacylglycero)phosphate (BMP), ...
Molecular Machines that Facilitate Bacterial Outer Membrane Protein Biogenesis [0.03%]
促进细菌外膜蛋白生物发生的分子机器
Matthew Thomas Doyle,Harris D Bernstein
Matthew Thomas Doyle
Almost all outer membrane proteins (OMPs) in Gram-negative bacteria contain a β-barrel domain that spans the outer membrane (OM). To reach the OM, OMPs must be translocated across the inner membrane by the Sec machinery, transported across...
Natural and Engineered Guide RNA-directed Transposition with CRISPR-Associated Tn7-like Transposons [0.03%]
具有CRISPR相关Tn7样转座酶的gRNA引导的自然和工程化转座子元件
Shan-Chi Hsieh,Joseph E Peters
Shan-Chi Hsieh
CRISPR-Cas (clustered regularly interspaced short palindromic repeats-CRISPR-associated nuclease) defense systems have been naturally coopted for guide RNA-directed transposition on multiple occasions. In all cases, cooption occurred with d...
How Natural Enzymes and Synthetic Ribozymes Generate Methylated Nucleotides in RNA [0.03%]
天然酶和人工核酶如何在RNA中生成甲基化核苷酸
Claudia Höbartner,Katherine E Bohnsack,Markus T Bohnsack
Claudia Höbartner
Methylation of RNA nucleotides represents an important layer of gene expression regulation, and perturbation of the RNA methylome is associated with pathophysiology. In cells, RNA methylations are installed by RNA methyltransferases (RNMTs)...
Kaitlyn R Browning,Houra Merrikh
Kaitlyn R Browning
DNA replication and transcription occur in all living cells across all domains of life. Both essential processes occur simultaneously on the same template, leading to conflicts between the macromolecular machines that perform these function...
Maria Falkenberg,Nils-Göran Larsson,Claes M Gustafsson
Maria Falkenberg
Mammalian mitochondrial DNA (mtDNA) is replicated and transcribed by phage-like DNA and RNA polymerases, and our understanding of these processes has progressed substantially over the last several decades. Molecular mechanisms have been elu...
Hemmo Meyer,Bojana Kravic
Hemmo Meyer
Lysosomes are the degradative endpoints of material delivered by endocytosis and autophagy and are therefore particularly prone to damage. Membrane permeabilization or full rupture of lysosomal or late endosomal compartments is highly delet...
Joseph M Paggi,Ayush Pandit,Ron O Dror
Joseph M Paggi
Molecular docking has become an essential part of a structural biologist's and medicinal chemist's toolkits. Given a chemical compound and the three-dimensional structure of a molecular target-for example, a protein-docking methods fit the ...