Small-Molecule-Based Fluorescent Sensors for Selective Detection of Reactive Oxygen Species in Biological Systems [0.03%]
基于小分子的荧光传感器选择性检测生物系统中的活性氧物种
Xiaoyu Bai,Kenneth King-Hei Ng,Jun Jacob Hu et al.
Xiaoyu Bai et al.
Reactive oxygen species (ROS) encompass a collection of intricately linked chemical entities characterized by individually distinct physicochemical properties and biological reactivities. Although excessive ROS generation is well known to u...
Christopher M Dobson
Christopher M Dobson
Over the past six decades, steadily increasing progress in the application of the principles and techniques of the physical sciences to the study of biological systems has led to remarkable insights into the molecular basis of life. Of part...
Horizontal Cell Biology: Monitoring Global Changes of Protein Interaction States with the Proteome-Wide Cellular Thermal Shift Assay (CETSA) [0.03%]
横向细胞生物学：利用基于细胞热休克蛋白组分析（CETSA）监测蛋白质相互作用的全局变化
Lingyun Dai,Nayana Prabhu,Liang Ying Yu et al.
Lingyun Dai et al.
The cellular thermal shift assay (CETSA) is a biophysical technique allowing direct studies of ligand binding to proteins in cells and tissues. The proteome-wide implementation of CETSA with mass spectrometry detection (MS-CETSA) has now be...
The BRCA Tumor Suppressor Network in Chromosome Damage Repair by Homologous Recombination [0.03%]
BRCA同源重组修复染色体损伤的肿瘤抑制网络
Weixing Zhao,Claudia Wiese,Youngho Kwon et al.
Weixing Zhao et al.
Mutations in the BRCA1 and BRCA2 genes predispose afflicted individuals to breast, ovarian, and other cancers. The BRCA-encoded products form complexes with other tumor suppressor proteins and with the recombinase enzyme RAD51 to mediate ch...
Lai-Xi Wang,Xin Tong,Chao Li et al.
Lai-Xi Wang et al.
Antibodies are immunoglobulins that play essential roles in immune systems. All antibodies are glycoproteins that carry at least one or more conserved N-linked oligosaccharides (N-glycans) at the Fc domain. Many studies have demonstrated th...
Jaime Vaquer-Alicea,Marc I Diamond
Jaime Vaquer-Alicea
Most common neurodegenerative diseases feature deposition of protein amyloids and degeneration of brain networks. Amyloids are ordered protein assemblies that can act as templates for their own replication through monomer addition. Evidence...
Gary J Doherty,Michele Petruzzelli,Emma Beddowes et al.
Gary J Doherty et al.
The complexity of human cancer underlies its devastating clinical consequences. Drugs designed to target the genetic alterations that drive cancer have improved the outcome for many patients, but not the majority of them. Here, we review th...
Jani Reddy Bolla,Mark T Agasid,Shahid Mehmood et al.
Jani Reddy Bolla et al.
Membrane proteins that exist in lipid bilayers are not isolated molecular entities. The lipid molecules that surround them play crucial roles in maintaining their full structural and functional integrity. Research directed at investigating ...
Werner Kühlbrandt
Werner Kühlbrandt
F1Fo ATP synthases produce most of the ATP in the cell. F-type ATP synthases have been investigated for more than 50 years, but a full understanding of their molecular mechanisms has become possible only with the recent structures of comple...
Evaluating and Enhancing Target Specificity of Gene-Editing Nucleases and Deaminases [0.03%]
评估和增强基因编辑核酸酶和脱氨酶的靶点特异性
Daesik Kim,Kevin Luk,Scot A Wolfe et al.
Daesik Kim et al.
Programmable nucleases and deaminases, which include zinc-finger nucleases, transcription activator-like effector nucleases, CRISPR RNA-guided nucleases, and RNA-guided base editors, are now widely employed for the targeted modification of ...