Ultrastructure of dopaminergic varicosities revealed by cryo-correlative light and electron microscopy [0.03%]
cryo-correlative光镜和电子显微镜技术揭示多巴胺神经元突触小体的精细结构
Matthew Domenic Lycas,Dustin R Morado,Ulrik Gether et al.
Matthew Domenic Lycas et al.
Dopaminergic neurons are fundamental in governing motivation, movement, and many aspects of cognition. The targeted modulation of dopaminergic signaling serves as a cornerstone in developing therapeutic interventions for conditions such as ...
Time-resolved tmFRET reveals GTP-coupled conformational changes in Mfn1 [0.03%]
时间分辨的tmFRET显示Mfn1中与GTP结合相关的构象变化
Sophie M Hurwitz,William N Zagotta,Sharona E Gordon et al.
Sophie M Hurwitz et al.
Outer mitochondrial membrane fusion is mediated by the mitofusin paralogs Mfn1 and Mfn2. Nucleotide-driven self-assembly and conformational changes are required for regulated membrane fusion activity, but the allosteric mechanisms remain en...
The environmental stress response regulates biophysics of the cytoplasm and survival in quiescence [0.03%]
环境压力反应调节静止状态下的细胞浆生物物理性质和生存能力
Lorena Kronig,Carmen A Weber,Pablo Aurelio Gómez-García et al.
Lorena Kronig et al.
All organisms employ strategies to cope with changing environmental conditions. In budding yeast, nutrient deprivation induces a reversible non-proliferative state known as quiescence, characterized by extensive remodeling of gene expressio...
Pulling the strings: Contractile VE-cadherin junctions to stabilize angiogenic sprouts [0.03%]
牵线搭桥：收缩型血管内皮钙黏蛋白连接稳定新生血管芽丝
Stephan Huveneers
Stephan Huveneers
Dynamic yet stable endothelial interactions during angiogenesis remain poorly understood despite their importance in development and regeneration. Mayo et al. (https://doi.org/10.1083/jcb.202503146) identify Scribble and myosin-1c as key re...
Regulation of the Cell Wall Integrity pathway at the contact site between mating partners in yeast [0.03%]
调控芽殖酵母交配偶联体结合位点的细胞壁完整性途径
Erin R Curtis,Aarshi Jain,Daniel J Lew
Erin R Curtis
The fungal cell wall is constantly remodeled to allow cell growth, but any holes in the cell wall would lead to catastrophic lysis. The "Cell Wall Integrity" pathway (CWI) detects cell wall defects and promotes cell wall thickening or repai...
Divergent Rickettsia species exhibit distinct mechanisms of actin-based motility [0.03%]
分歧立克次氏体物种表现出不同类型的基于肌动蛋白的运动机制
Meghan C Bacher,Julie E Choe,Jiawen Jiang et al.
Meghan C Bacher et al.
Many Rickettsia species undergo actin-based motility to promote cell-cell spread during infection. Rickettsial genomes often encode two motility effectors, RickA and Sca2, which in the spotted fever group I species, Rickettsia parkeri act b...
Scrib organizes cortical actomyosin clusters to maintain adherens junctions and angiogenic sprouting [0.03%]
Scrib通过组织皮层肌动蛋白肌球蛋白聚集体来维持粘着斑连接和血管生成分支行为
Lakyn N Mayo,Fiona Duong,Ana Mompeón et al.
Lakyn N Mayo et al.
Spatiotemporal control of adherens junction fluidity and integrity is critical for angiogenesis, but underlying mechanisms are incompletely understood. To identify unappreciated regulators of endothelial adherens junctions, we performed VE-...
Actomyosin contractility is a potent suppressor of mesoderm induction by human pluripotent stem cells [0.03%]
肌动蛋白力学张力可抑制人多能干细胞向中胚层的诱导分化
Loic Fort,Vaishna Vamadevan,Wenjun Wang et al.
Loic Fort et al.
Activation of WNT signaling in human pluripotent stem cells efficiently drives lateral mesoderm specification and subsequent cardiomyocyte differentiation. Stabilization of the WNT effector β-catenin induces mesodermal genes such as TBXT (...
Florian Fröhlich
Florian Fröhlich
Bridge-like lipid-transport protein 2 (BLTP2) transfers lipids at membrane contact sites, but its precise localization is unclear. Dziurdzik et al. and Dai et al. identify and characterize a conserved contact site between the endoplasmic re...
Morphological control of gut lumen access shapes enteroendoceine cell function [0.03%]
肠腔进入的形态控制塑造了肠内分泌细胞的功能
Qingyin Qian,Ryusuke Niwa,Yu-Ichiro Nakajima
Qingyin Qian
In this issue, Yu et al. (https://doi.org/10.1083/jcb.202506084) uncover a genetic mechanism that determines whether enteroendocrine cells access the gut lumen. Their findings link regional transcriptional control to epithelial integration ...