Detection of an ABCA1 variant associated with type 2 diabetes mellitus susceptibility for biochemistry and genetic laboratory courses [0.03%]
一种与2型糖尿病易感性相关的ABCA1变异的检测——用于生化及遗传实验课程
M Legorreta-Herrera,N A Mosqueda-Romo,F Hernández-Clemente et al.
M Legorreta-Herrera et al.
We selected diabetes mellitus for this laboratory exercise to provide students with an explicit model for scientific research concerning the association between the R230C polymorphism and susceptibility to type 2 diabetes mellitus, which is...
Comparative modeling of proteins: a method for engaging students' interest in bioinformatics tools [0.03%]
蛋白质的比较模型构建：一种激发学生对生物信息工具兴趣的方法
Fernanda Badotti,Alan Sales Barbosa,André Luiz Martins Reis et al.
Fernanda Badotti et al.
The huge increase in data being produced in the genomic era has produced a need to incorporate computers into the research process. Sequence generation, its subsequent storage, interpretation, and analysis are now entirely computer-dependen...
Raising environmental awareness through applied biochemistry laboratory experiments [0.03%]
通过应用生物化学实验进行环保意识教育
S Salman Ashraf
S Salman Ashraf
Our environment is under constant pressure and threat from various sources of pollution. Science students, in particular chemistry students, must not only be made aware of these issues, but also be taught that chemistry (and science) can pr...
Introduction: promoting concept driven teaching strategies in biochemistry and molecular biology [0.03%]
绪论：促进生物化学及分子生物学的概念驱动教学策略
Carla Mattos,Margaret Johnson,Hal White et al.
Carla Mattos et al.
Environmental regulation of plant gene expression: an RT-qPCR laboratory project for an upper-level undergraduate biochemistry or molecular biology course [0.03%]
环境调节植物基因表达：一项适用于高年级本科生生化或分子生物学课程的RT-qPCR实验室项目
Garrett J Eickelberg,Alison J Fisher
Garrett J Eickelberg
We present a novel laboratory project employing "real-time" RT-qPCR to measure the effect of environment on the expression of the FLOWERING LOCUS C gene, a key regulator of floral timing in Arabidopsis thaliana plants. The project requires ...
Studying epigenetic DNA modifications in undergraduate laboratories using complementary bioinformatic and molecular approaches [0.03%]
结合生物信息和分子方法在大学生化实验中研究表观遗传DNA修饰现象
Kevin T Militello
Kevin T Militello
Epigenetic inheritance is the inheritance of genetic information that is not based on DNA sequence alone. One type of epigenetic information that has come to the forefront in the last few years is modified DNA bases. The most common modifie...
Affordable hands-on DNA sequencing and genotyping: an exercise for teaching DNA analysis to undergraduates [0.03%]
一种针对本科生的DNA分析教学实验：亲民又实用的测序与基因型鉴定方法
Kushani Shah,Shelby Thomas,Arnold Stein
Kushani Shah
In this report, we describe a 5-week laboratory exercise for undergraduate biology and biochemistry students in which students learn to sequence DNA and to genotype their DNA for selected single nucleotide polymorphisms (SNPs). Students use...
Problem-solving test: conditional gene targeting using the Cre/loxP recombination system [0.03%]
利用Cre/LoxP重组系统进行条件基因敲除的问题测试
József Szeberényi
József Szeberényi
Terms to be familiar with before you start to solve the test: gene targeting, knock-out mutation, bacteriophage, complementary base-pairing, homologous recombination, deletion, transgenic organisms, promoter, polyadenylation element, transg...