Isolation of progenitor cells from GFP-transgenic pigs and transplantation to the retina of allorecipients [0.03%]
从GFP转基因猪分离前体细胞并移植到同种异体受体视网膜中
Henry Klassen,Karin Warfvinge,Philip H Schwartz et al.
Henry Klassen et al.
Work in rodents has demonstrated that progenitor transplantation can achieve limited photoreceptor replacement in the mammalian retina; however, replication of these findings on a clinically relevant scale requires a large animal model. To ...
Development and validation of a highly efficient protocol of porcine somatic cloning using preovulatory embryo transfer in peripubertal gilts [0.03%]
一种高效克隆猪的方案及其在初情期母猪受体中利用排卵前胚胎移植的验证
Björn Petersen,Andrea Lucas-Hahn,Marianne Oropeza et al.
Björn Petersen et al.
The efficiency of porcine somatic nuclear transfer (born piglets/transferred embryos) is low. Here, we report a highly efficient protocol using peripubertal gilts as recipients synchronized to ovulate approximately 24 h after transfer of cl...
Transgenic-cloned pigs systemically expressing red fluorescent protein, Kusabira-Orange [0.03%]
全身表达红色荧光蛋白Kusabira-Orange的转基因克隆猪系统
Hitomi Matsunari,Masafumi Onodera,Norihiro Tada et al.
Hitomi Matsunari et al.
Genetically engineered pigs with cell markers such as fluorescent proteins are highly useful in lines of research that include the tracking of transplanted cells or tissues. In this study, we produced transgenic-cloned pigs carrying a gene ...
Knockout serum replacement (KSR) has a suppressive effect on Sendai virus-mediated transduction of cynomolgus ES cells [0.03%]
补体激活培养基中的小鼠胎肝白蛋白和KO-SCF对食蟹猴ES细胞Sendai病毒介导的基因修饰效率的影响研究
Yukiko Kishi,Makoto Inoue,Yujiro Tanaka et al.
Yukiko Kishi et al.
Sendai virus (SeV) vectors can introduce foreign genes efficiently and stably into primate embryonic stem (ES) cells. For the application of these cells, the control of transgene expression is important. Cynomolgus ES cells transduced with ...
Caffeine treatment prevents age-related changes in ovine oocytes and increases cell numbers in blastocysts produced by somatic cell nuclear transfer [0.03%]
Joon-Hee Lee,Keith H S Campbell
Joon-Hee Lee
Maturation-promoting factor (MPF) and mitogen-activated protein kinase (MAPK) are key regulators of both meiotic and mitotic cycles. Oocytes arrested at metaphase of the second meiotic division (MII) contain high levels of both kinases; how...
Bioreactor cultivation enhances NTEB formation and differentiation of NTES cells into cardiomyocytes [0.03%]
生物反应器培养促进NTEB形成及NTEB干细胞向心肌细胞分化
Shuanghong Lü,Sheng Liu,Wenjun He et al.
Shuanghong Lü et al.
Autogenic embryonic stem cells established from somatic cell nuclear transfer (SCNT) embryos have been proposed as unlimited cell sources for cell transplantation-based treatment of many genetic and degenerative diseases, which can eliminat...
Protocol for the recovery of in vivo matured canine oocytes based on once daily measurement of serum progesterone [0.03%]
基于每天一次测量血清孕酮的大狗体内成熟卵母细胞的回收方案
Mohammad Shamim Hossein,Yeon Woo Jeong,Sue Kim et al.
Mohammad Shamim Hossein et al.
The collection of in vivo matured canine oocytes relies on the accurate prediction of ovulation. The present study was designed to develop a protocol for the recovery of in vivo matured canine oocytes based on once daily measurements of ser...
Activation of human embryonic gene expression in cytoplasmic hybrid embryos constructed between bovine oocytes and human fibroblasts [0.03%]
人成纤维细胞与牛卵母细胞重组的嵌合胚胎中人端粒酶反转录酶基因启动子的人类胚胎基因表达激活
Feng Li,Henhua Cao,Quanjun Zhang et al.
Feng Li et al.
Cross-species somatic all number transfer (SCNT) provides a potential solution to overcome the problem of oocyte shortage for therapeutic cloning. To further characterize the system, we constructed cytoplasm hybrid embryos between bovine oo...
The inhibitory role of stromal cell mesenchyme on human embryonic stem cell hepatocyte differentiation is overcome by Wnt3a treatment [0.03%]
Wnt3a处理可克服基质细胞间充质组织对人胚胎干细胞向肝细胞分化的抑制作用
Judy Fletcher,Wei Cui,Kay Samuel et al.
Judy Fletcher et al.
Pluripotent stem cells are derived from the inner cell mass of preimplantation embryos, and display the ability of the embryonic founder cells by forming all three germ lineages in vitro. It is well established that the cellular niche plays...
High hydrostatic pressure treatment of porcine oocytes before handmade cloning improves developmental competence and cryosurvival [0.03%]
高压处理可提高手工克隆猪卵的发育能力和冻存后存活率
Yutao Du,Lin Lin,Mette Schmidt et al.
Yutao Du et al.
An innovative technique, called the high hydrostatic pressure (HHP) treatment, has been recently reported to improve the cryosurvival of gametes or embryos in certain mammalian species. The aim of the present study was to investigate the in...