Data-driven extraction of human kinase-substrate relationships from omics datasets [0.03%]
基于组学数据集的人类激酶-底物关系的提取
Benjamin Dominik Maier,Borgthor Petursson,Alessandro Lussana et al.
Benjamin Dominik Maier et al.
Phosphorylation forms an important part of the signalling system that cells use for decision making and regulation of processes such as cell division and differentiation. In human, >90% of identified phosphosites don't have annotations rega...
Native Top-down analysis of membrane protein complexes directly from in vitro and native membranes [0.03%]
基于体外和天然膜的膜蛋白复合物的本征自上而下分析法
Wonhyeuk Jung,Aniruddha Panda,Jaywon Lee et al.
Wonhyeuk Jung et al.
Macromolecular organization of proteins and lipids in cellular membranes is fundamental to cell functionality. Recent advances in native mass spectrometry (nMS) have established it as a key analytical tool for capturing these associations. ...
Chronic alcohol consumption reprograms hepatic metabolism through organelle-specific acetylation in mice [0.03%]
慢性酒精摄入通过器官特异性乙酰化重构小鼠肝脏代谢
Mirjavid Aghayev,Megan R McMullen,Serguei Ilchenko et al.
Mirjavid Aghayev et al.
Post-translational acetylation of proteins by acetyl-CoA is a crucial regulator of proteostasis and substrate metabolism. Ethanol metabolism in the liver induces protein accumulation, acetylation and metabolic disruption. While acetylation ...
Theileria annulata Hijacks Host Signaling: Integrated Phosphoproteomics and transcriptomics Unveils ERK1/2 as a Central Regulator of Host Transcription Factors [0.03%]
théilerialannulata干扰宿主信号转导：整合的磷酸蛋白质组学和转录组学揭示ERK1/2是宿主转录因子的核心调控因素
Debabrata Dandasena,Vengatachala Moorthy A,Akash Suresh et al.
Debabrata Dandasena et al.
THEILERIA: transformed bovine leukocytes exhibit cancer-like characteristics, but the molecular mechanisms driving these transformations remain unclear. This study provides the first comprehensive phosphoproteomic analysis of both host and ...
Proteomics and phosphoproteomics revealed dysregulated kinases and potential therapy for liver fibrosis [0.03%]
蛋白质组和磷酸化蛋白质组揭示了肝纤维化中失调的激酶及潜在治疗靶点
Xinyu Cheng,Li Kang,Jinfang Liu et al.
Xinyu Cheng et al.
Liver fibrosis is the initial stage of most liver diseases, and it is also a pathological process involving the liver in the late stages of many metabolic diseases. Therefore, it is important to systematically understand the pathological me...
Nina A Khristenko,Konstantin O Nagornov,Camille Garcia et al.
Nina A Khristenko et al.
Therapeutic antibodies, primarily immunoglobulin G-based monoclonal antibodies, are developed to treat cancer, autoimmune disorders, and infectious diseases. Their large size, structural complexity, and heterogeneity pose significant analyt...
Temporal wheat proteome remodeling by deoxynivalenol reveals novel detoxification signatures and strategies across cultivars [0.03%]
脱氧雪腐镰刀菌烯醇引起的小麦不同品种间时间依赖性蛋白组重编程揭示新的解毒标志物和策略
Reid Buchanan,Kholoud Shaban,Boyan Liu et al.
Reid Buchanan et al.
Fusarium head blight (FHB) is a globally devastating fungal disease resulting in reduced grain yield and quality, along with contamination of grains with dangerous mycotoxins. Consumption of such mycotoxins by humans through processed food ...
Laser-Induced Rehydration of Cryo-Landed Proteins Restores Native Structure [0.03%]
激光诱导的冷冻着陆蛋白的再水化可恢复其天然结构
Keaton L Mertz,Jordahl,Colin A Hemme et al.
Keaton L Mertz et al.
The use of native mass spectrometry (MS) to land biological molecules for subsequent cryogenic electron microscopy (cryoEM) imaging and three-dimensional reconstruction has gained momentum in recent years as a means to overcome longstanding...
Benchmarking SILAC Proteomics Workflows and Data Analysis Platforms [0.03%]
用于评估稳定同位素标记亲和纯化联合质谱的工作流程及数据分析平台性能
Ashley M Frankenfield,Kevin Yang,Wan Nur Atiqah Binti Mazli et al.
Ashley M Frankenfield et al.
Stable isotope labeling by amino acids in cell culture (SILAC) is a powerful metabolic labeling technique with broad applications and various study designs. SILAC proteomics relies on the accurate identification and quantification of all is...
Hirokazu Yagi,Taiki Saito,Shih-Yun Guu et al.
Hirokazu Yagi et al.
We characterized the N-glycosylation profiles of anhydrobiotic tardigrades, R. varieornatus and H. exemplaris, identifying high-mannose, paucimannose, and complex-type oligosaccharides, while hybrid-type glycans were undetectable. Notably, ...