Capture, Release, and Identification of Newly Synthesized Proteins for Improved Profiling of Functional Translatomes [0.03%]
新合成蛋白质的捕获、释放和识别以改善功能性转录组谱分析
Nancy J Phillips,Bala M Vinaithirthan,Juan A Oses-Prieto et al.
Nancy J Phillips et al.
New protein synthesis is regulated both at the level of mRNA transcription and translation. RNA-Seq is effective at measuring levels of mRNA expression, but techniques to monitor mRNA translation are much more limited. Previously, we report...
TEAD Proteins Associate With DNA Repair Proteins to Facilitate Cellular Recovery From DNA Damage [0.03%]
TEAD蛋白与DNA修复蛋白结合以促进细胞从DNA损伤中恢复
Philamer C Calses,Victoria C Pham,Alissa D Guarnaccia et al.
Philamer C Calses et al.
Transcriptional enhanced associate domain family members 1 to 4 (TEADs) are a family of four transcription factors and the major transcriptional effectors of the Hippo pathway. In order to activate transcription, TEADs rely on interactions ...
In-Cell Chemical Crosslinking Identifies Hotspots for SQSTM-1/p62-IκBα Interaction That Underscore a Critical Role of p62 in Limiting NF-κB Activation Through IκBα Stabilization [0.03%]
细胞内化学交联确定SQSTM-1/p62-IκBα相互作用热点，强调p62通过IκBα稳定来限制NF-κB激活的关键作用
Yi Liu,Michael J Trnka,Liang He et al.
Yi Liu et al.
We have previously documented that in liver cells, the multifunctional protein scaffold p62/SQSTM1 is closely associated with IκBα, an inhibitor of the transcriptional activator NF-κB. Such an intimate p62-IκBα association we now docum...
Integrated Single-Cell (Phospho-)Protein and RNA Detection Uncovers Phenotypic Characteristics and Active Signal Transduction of Human Antibody-Secreting Cells [0.03%]
单细胞(磷酸化)蛋白质和RNA整合检测揭示了人抗体分泌细胞的表型特性和活性信号转导特性
Erik van Buijtenen,Wout Janssen,Paul Vink et al.
Erik van Buijtenen et al.
Single-cell technologies are currently widely applied to obtain a deeper understanding of the phenotype of single-cells in heterogenous mixtures. However, integrated multilayer approaches including simultaneous detection of mRNA, protein ex...
Quantification and Proteomic Characterization of β-Hydroxybutyrylation Modification in the Hearts of AMPKα2 Knockout Mice [0.03%]
AMPKα2基因敲除小鼠心脏组织中β-羟基丁酰化修饰的定量及蛋白质组特征分析
Wen-Jing Ding,Xue-Hui Li,Cong-Min Tang et al.
Wen-Jing Ding et al.
AMP-activated protein kinase alpha 2 (AMPKα2) regulates energy metabolism, protein synthesis, and glucolipid metabolism myocardial cells. Ketone bodies produced by fatty acid β-oxidation, especially β-hydroxybutyrate, are fatty energy-su...
Proteomics Investigation of Diverse Serological Patterns in COVID-19 [0.03%]
COVID-19多样血清学图谱的蛋白质组学研究
Xiao Liang,Rui Sun,Jing Wang et al.
Xiao Liang et al.
Serum antibodies IgM and IgG are elevated during Coronavirus Disease 2019 (COVID-19) to defend against viral attacks. Atypical results such as negative and abnormally high antibody expression were frequently observed whereas the underlying ...
Spatially Resolved Top-Down Proteomics of Tissue Sections Based on a Microfluidic Nanodroplet Sample Preparation Platform [0.03%]
基于微流控纳米液滴样品制备平台的组织切片空间解析自上而下的蛋白质组学
Yen-Chen Liao,James M Fulcher,David J Degnan et al.
Yen-Chen Liao et al.
Conventional proteomic approaches measure the averaged signal from mixed cell populations or bulk tissues, leading to the dilution of signals arising from subpopulations of cells that might serve as important biomarkers. Recent developments...
Lysine Succinylation of VBS Contributes to Sclerotia Development and Aflatoxin Biosynthesis in Aspergillus flavus [0.03%]
黄曲霉中VBS的苏式赖氨酸化有助于子座发育和黄曲霉素生物合成
Yu Wang,Mingkun Yang,Feng Ge et al.
Yu Wang et al.
Aspergillus flavus is a common saprophytic and pathogenic fungus, and its secondary metabolic pathways are one of the most highly characterized owing to its aflatoxin (AF) metabolite affecting global economic crops and human health. Differe...
Synchro-PASEF Allows Precursor-Specific Fragment Ion Extraction and Interference Removal in Data-Independent Acquisition [0.03%]
同步PASEF允许在数据独立获取中进行前体特异性片段离子提取和干扰去除
Patricia Skowronek,Florian Krohs,Markus Lubeck et al.
Patricia Skowronek et al.
Data-independent acquisition (DIA) methods have become increasingly popular in mass spectrometry-based proteomics because they enable continuous acquisition of fragment spectra for all precursors simultaneously. However, these advantages co...
HIV-1 Transcription Inhibitor 1E7-03 Decreases Nucleophosmin Phosphorylation [0.03%]
HIV-1转录抑制剂1E7-03降低核磷蛋白磷酸化
Xionghao Lin,Asrar Ahmad,Andrey I Ivanov et al.
Xionghao Lin et al.
Transcription activation of latent human immunodeficiency virus-1 (HIV-1) occurs due to HIV-1 rebound, the interruption of combination antiretroviral therapy, or development of drug resistance. Thus, novel HIV-1 inhibitors, targeting HIV-1 ...