Dynamic Proteomic Changes in Tumor and Immune Organs Reveal Systemic Immune Response to Tumor Development [0.03%]
肿瘤及免疫器官的蛋白组动态变化揭示机体对肿瘤发展的免疫响应机制
Zhike Li,Shuwen Liu,Zhouyong Gao et al.
Zhike Li et al.
In orthotopic mouse tumor models, tumor progression is a complex process, involving interactions among tumor cells, host cell-derived stromal cells, and immune cells. Much attention has been focused on the tumor and its tumor microenvironme...
Systematic Optimization of Automated Phosphopeptide Enrichment for High-Sensitivity Phosphoproteomics [0.03%]
用于高灵敏度磷酸蛋白质组学的自动化磷酸肽富集的系统优化方法研究
Patricia Bortel,Ilaria Piga,Claire Koenig et al.
Patricia Bortel et al.
Improving coverage, robustness, and sensitivity is crucial for routine phosphoproteomics analysis by single-shot liquid chromatography-tandem mass spectrometry (LC-MS/MS) from minimal peptide inputs. Here, we systematically optimized key ex...
Protein Interaction Map of APOBEC3 Enzyme Family Reveals Deamination-Independent Role in Cellular Function [0.03%]
APOBEC3酶族蛋白质相互作用图谱揭示了去氨化独立的细胞功能作用机制
Gwendolyn M Jang,Arun Kumar Annan Sudarsan,Arzhang Shayeganmehr et al.
Gwendolyn M Jang et al.
Human APOBEC3 enzymes are a family of single-stranded (ss)DNA and RNA cytidine deaminases that act as part of the intrinsic immunity against viruses and retroelements. These enzymes deaminate cytosine to form uracil which can functionally i...
Quantification of Adaptive Immune Responses Against Protein-Binding Interfaces in the Streptococcal M1 Protein [0.03%]
针对链球菌M1蛋白结合面的适应性免疫反应量化研究
Eva Torres-Sangiao,Lotta Happonen,Morizt Heusel et al.
Eva Torres-Sangiao et al.
Bacterial or viral antigens can contain subdominant protein regions that elicit weak antibody responses upon vaccination or infection although there is accumulating evidence that antibody responses against subdominant regions can enhance th...
An Automated and Fast Sample Preparation Workflow for Laser Microdissection Guided Ultrasensitive Proteomics [0.03%]
一种用于激光显微解剖引导的超灵敏蛋白质组学的自动化快速样品制备流程
Anuar Makhmut,Di Qin,David Hartlmayr et al.
Anuar Makhmut et al.
Spatial tissue proteomics integrating whole-slide imaging, laser microdissection, and ultrasensitive mass spectrometry is a powerful approach to link cellular phenotypes to functional proteome states in (patho)physiology. To be applicable t...
High-Throughput Antigen Microarray Identifies Longitudinal Prognostic Autoantibody for Chemoimmunotherapy in Advanced Non-Small Cell Lung Cancer [0.03%]
高通量抗原微阵列鉴定出化疗联合免疫疗法在晚期非小细胞肺癌中的纵向预后自身抗体
Liyuan Dai,Qiaoyun Tan,Lin Li et al.
Liyuan Dai et al.
Chemoimmunotherapy has evolved as a standard treatment for advanced non-small cell lung cancer (aNSCLC). However, inevitable drug resistance has limited its efficacy, highlighting the urgent need for biomarkers of chemoimmunotherapy. A thre...
Proteomic Profiling of Muscular Adaptations to Short-Term Concentric Versus Eccentric Exercise Training in Humans [0.03%]
人类短期离心运动训练与向心运动训练肌肉适应性的蛋白质谱分析
Jiawei Du,Hezhang Yun,Hongsheng Wang et al.
Jiawei Du et al.
The molecular mechanisms underlying muscular adaptations to concentric (CON) and eccentric (ECC) exercise training have been extensively explored. However, most previous studies have focused on specifically selected proteins, thus, unable t...
Galectin-4 Antimicrobial Activity Primarily Occurs Through its C-Terminal Domain [0.03%]
半乳糖凝集素-4的抗菌活性主要通过其C端域发生
Hau-Ming Jan,Shang-Chuen Wu,Carter J Stowell et al.
Hau-Ming Jan et al.
Although immune tolerance evolved to reduce reactivity with self, it creates a gap in the adaptive immune response against microbes that decorate themselves in self-like antigens. This is particularly apparent with carbohydrate-based blood ...
Ribosome Profiling and Mass Spectrometry Reveal Widespread Mitochondrial Translation Defects in a Striatal Cell Model of Huntington Disease [0.03%]
核糖体分析和质谱法揭示了亨廷顿病纹状细胞模型中普遍存在线粒体翻译缺陷
Sunayana Dagar,Manish Sharma,George Tsaprailis et al.
Sunayana Dagar et al.
Huntington disease (HD) is caused by an expanded polyglutamine mutation in huntingtin (mHTT) that promotes prominent atrophy in the striatum and subsequent psychiatric, cognitive deficits, and choreiform movements. Multiple lines of evidenc...
Universal Pretreatment Development for Low-input Proteomics Using Lauryl Maltose Neopentyl Glycol [0.03%]
使用月桂基麦芽糖新戊二醇进行低投入蛋白质组学的通用预处理开发
Ryo Konno,Masaki Ishikawa,Daisuke Nakajima et al.
Ryo Konno et al.
In recent years, there has been a growing demand for low-input proteomics, particularly in the context of single-cell proteomics (SCP). In this study, we have developed a lauryl maltose neopentyl glycol (LMNG)-assisted sample preparation (L...