MIB2 Functions in Oocyte Meiosis by Modulating Chromatin Configuration [0.03%]
MIB2通过调节染色质构象在卵母细胞减数分裂中的作用
Yifei Jin,Guangyi Sun,Jiashuo Li et al.
Yifei Jin et al.
Chromatin configuration serves as a principal indicator of GV (germinal vesicle)-stage oocyte quality. However, the underlying mechanisms governing the chromatin configuration transition from NSN (non-surrounded nucleolus) to SN (surrounded...
A Carrier-Based Quantitative Proteomics Method Applied to Biomarker Discovery in Pericardial Fluid [0.03%]
基于载体的定量蛋白质组学方法在心包液生物标志物发现中的应用
Amanda J Campbell,Samir Cakar,Nicolai B Palstrøm et al.
Amanda J Campbell et al.
Data-dependent liquid chromatography tandem mass spectrometry is challenged by the large concentration range of proteins in plasma and related fluids. We adapted the SCoPE method from single-cell proteomics to pericardial fluid, where a myo...
Enhanced in situ spatial proteomics by effective combination of MALDI imaging and LC-MS/MS [0.03%]
有效结合MALDI成像和LC-MS/MS提高原位空间蛋白质组学水平
Frederike Schäfer,Archana Tomar,Shogo Sato et al.
Frederike Schäfer et al.
Highly specialized cells are fundamental for proper functioning of complex organs. Variations in cell-type specific gene expression and protein composition have been linked to a variety of diseases. Investigation of the distinctive molecula...
Quantitative phosphoproteomic profiling of mouse sperm maturation in epididymis revealed kinases important for sperm motility [0.03%]
定量分析小鼠附睾精子成熟过程中磷酸化蛋白质组的动态变化并筛选影响精子运动性的关键激酶
Xiangzheng Zhang,Haixia Tu,Xin Zhou et al.
Xiangzheng Zhang et al.
Transcriptionally and translationally silent sperm undergo functional maturation during epididymis traverse, which provides sperm ability to move and is crucial for successful fertilization. However, the molecular mechanisms governing sperm...
Proximity Labeling Proteomics Reveals Kv1.3 Potassium Channel Immune Interactors in Microglia [0.03%]
邻位连接蛋白组学揭示小胶质细胞中Kv1.3钾通道的免疫互作蛋白
Christine A Bowen,Hai M Nguyen,Young Lin et al.
Christine A Bowen et al.
Microglia are resident immune cells of the brain and regulate its inflammatory state. In neurodegenerative diseases, microglia transition from a homeostatic state to a state referred to as disease-associated microglia (DAM). DAM express hig...
DIA-Based Phosphoproteomics Identifies Early Phosphorylation Events in Response to EGTA and Mannitol in Arabidopsis [0.03%]
基于DIA的磷酸化蛋白质组学鉴定拟南芥对EGTA和甘露醇响应的早期磷酸化事件
Tian Sang,Chin-Wen Chen,Zhen Lin et al.
Tian Sang et al.
Osmotic stress significantly hampers plant growth and crop yields, emphasizing the need for a thorough comprehension of the underlying molecular responses. Previous research has demonstrated that osmotic stress rapidly induces calcium influ...
A Targeted LC-MRM3 Proteomic Approach for the Diagnosis of SARS-CoV-2 Infection in Nasopharyngeal Swabs [0.03%]
鼻咽拭子标本中SARS-CoV-2感染诊断的靶向液相色谱多反应监测三重法蛋白组学方法
Nicolas Drouin,Hyung L Elfrink,Stefan A Boers et al.
Nicolas Drouin et al.
Since its first appearance, severe acute respiratory syndrome coronavirus 2 quickly spread around the world and the lack of adequate PCR testing capacities, especially during the early pandemic, led the scientific community to explore new a...
Chemo-phosphoproteomic profiling with ATR inhibitors berzosertib and gartisertib uncovers new biomarkers and DNA damage response regulators [0.03%]
ATR抑制剂berzosertib和gartisertib的化疗磷蛋白质谱分析揭示新的生物标志物和DNA损伤响应调节因子
Rathan Jadav,Florian Weiland,Sylvie M Noordermeer et al.
Rathan Jadav et al.
The ATR kinase protects cells against DNA damage and replication stress and represents a promising anti-cancer drug target. The ATR inhibitors (ATRi) berzosertib and gartisertib are both in clinical trials for the treatment of advanced soli...
Data-independent acquisition: A milestone and prospect in clinical mass spectrometry-based proteomics [0.03%]
数据无关型采集：临床质谱蛋白质组学中的一个里程碑与展望
Klemens Fröhlich,Matthias Fahrner,Eva Brombacher et al.
Klemens Fröhlich et al.
Data-independent acquisition (DIA) has revolutionized the field of mass spectrometry (MS)-based proteomics over the past few years. DIA stands out for its ability to systematically sample all peptides in a given mass-to-charge range, allowi...
Profiling Proteins and Phosphorylation Sites During T Cell Activation Using an Integrated Thermal Shift Assay [0.03%]
利用整合热力学测定法分析T细胞活化过程中的蛋白质及磷酸化位点
Brandon M Gassaway,Edward L Huttlin,Emily M Huntsman et al.
Brandon M Gassaway et al.
T cell activation is a complex biological process of naive cells maturing into effector cells. Proteomic and phospho-proteomic approaches have provided critical insights into this process, yet it is not always clear how changes in individua...