The Plastidial Protein Acetyltransferase GNAT1 Forms a Complex With GNAT2, yet Their Interaction Is Dispensable for State Transitions [0.03%]
叶绿体蛋白乙酰转移酶GNAT1与GNAT2形成复合体，但两者互作并非进行状态转换的必需因素
Annika Brünje,Magdalena Füßl,Jürgen Eirich et al.
Annika Brünje et al.
Protein N-acetylation is one of the most abundant co- and post-translational modifications in eukaryotes, extending its occurrence to chloroplasts within vascular plants. Recently, a novel plastidial enzyme family comprising eight acetyltra...
NovoBoard: A Comprehensive Framework for Evaluating the False Discovery Rate and Accuracy of De Novo Peptide Sequencing [0.03%]
NovoBoard：一种全面评估从头蛋白序列鉴定的假发现率和准确性的框架
Ngoc Hieu Tran,Rui Qiao,Zeping Mao et al.
Ngoc Hieu Tran et al.
De novo peptide sequencing is one of the most fundamental research areas in mass spectrometry-based proteomics. Many methods have often been evaluated using a couple of simple metrics that do not fully reflect their overall performance. Mor...
Light Changes Promote Distinct Responses of Plastid Protein Acetylation Marks [0.03%]
光变化促进质体蛋白乙酰化标志的特异性响应
Jürgen Eirich,Jean-Baptiste Boyer,Laura Armbruster et al.
Jürgen Eirich et al.
Protein acetylation is a key co- and post-translational modification. However, how different types of acetylation respond to environmental stress is still unknown. To address this, we investigated the role of a member of the newly discovere...
Comprehensive Proteomic Analysis Reveals Distinct Features and a Diagnostic Biomarker Panel for Early Pregnancy Loss in Histological Subtypes [0.03%]
全面蛋白质组分析揭示了.histological亚型早期妊娠丢失的特征和诊断生物标志物谱面板
Yating Zhao,Yingjiqiong Liang,Luya Cai et al.
Yating Zhao et al.
Early pregnancy loss (EPL) is a common event in human reproduction and is classified into histological subtypes such as hydropic abortion (HA) and hydatidiform moles, including complete hydatidiform moles (CHMs) and partial hydatidiform mol...
A Nitrogen-specific Interactome Analysis Sheds Light on the Role of the SnRK1 and TOR Kinases in Plant Nitrogen Signaling [0.03%]
氮特异的蛋白质相互作用分析揭示了SnRK1和TOR激酶在植物氮信号传导中的作用
Freya Persyn,Wouter Smagghe,Dominique Eeckhout et al.
Freya Persyn et al.
Nitrogen (N) is of utmost importance for plant growth and development. Multiple studies have shown that N signaling is tightly coupled with carbon (C) levels, but the interplay between C/N metabolism and growth remains largely an enigma. No...
Proteome-Scale Tissue Mapping Using Mass Spectrometry Based on Label-Free and Multiplexed Workflows [0.03%]
基于无标签和多重标记的质谱技术在蛋白质组水平上的组织mapping研究
Yumi Kwon,Jongmin Woo,Fengchao Yu et al.
Yumi Kwon et al.
Multiplexed bimolecular profiling of tissue microenvironment, or spatial omics, can provide deep insight into cellular compositions and interactions in healthy and diseased tissues. Proteome-scale tissue mapping, which aims to unbiasedly vi...
Insights Into Glycobiology and the Protein-Glycan Interactome Using Glycan Microarray Technologies [0.03%]
运用糖芯片技术研究糖生物学及蛋白质-多糖相互作用组学的见解
Jamie Heimburg-Molinaro,Akul Y Mehta,Catherine A Tilton et al.
Jamie Heimburg-Molinaro et al.
Glycans linked to proteins and lipids and also occurring in free forms have many functions, and these are partly elicited through specific interactions with glycan-binding proteins (GBPs). These include lectins, adhesins, toxins, hemaggluti...
Proteomic Heterogeneity of the Extracellular Matrix Identifies Histologic Subtype-Specific Fibroblast in Gastric Cancer [0.03%]
胃癌细胞外基质的蛋白质异质性识别出组织学亚型特异性成纤维细胞
Hyun Jin Lee,Yoonjin Kwak,Yun Suk Na et al.
Hyun Jin Lee et al.
Gastric cancer (GC) is a highly heterogeneous disease regarding histologic features, genotypes, and molecular phenotypes. Here, we investigate extracellular matrix (ECM)-centric analysis, examining its association with histologic subtypes a...
metaExpertPro: A Computational Workflow for Metaproteomics Spectral Library Construction and Data-Independent Acquisition Mass Spectrometry Data Analysis [0.03%]
元蛋白质谱库构建和数据非依赖性获取质谱数据分析的计算工作流程-metaExpertPro：元蛋白质组学光谱库构建和数据无关型获取质谱数据分析的计算工作流
Yingying Sun,Ziyuan Xing,Shuang Liang et al.
Yingying Sun et al.
Analysis of large-scale data-independent acquisition mass spectrometry metaproteomics data remains a computational challenge. Here, we present a computational pipeline called metaExpertPro for metaproteomics data analysis. This pipeline enc...
Combining Data Independent Acquisition With Spike-In SILAC (DIA-SiS) Improves Proteome Coverage and Quantification [0.03%]
结合数据非依赖性采集与 spike-in SILAC(DIA-SiS)可提高蛋白质覆盖范围及定量准确性
Anna Sophie Welter,Maximilian Gerwien,Robert Kerridge et al.
Anna Sophie Welter et al.
Data-independent acquisition (DIA) is increasingly preferred over data-dependent acquisition due to its higher throughput and fewer missing values. Whereas data-dependent acquisition often uses stable isotope labeling to improve quantificat...