Receptor Kinase Signaling of BRI1 and SIRK1 Is Tightly Balanced by Their Interactomes as Revealed From Domain-Swap Chimaera in AE-MS Approaches [0.03%]
基于域置换嵌合体的邻近依赖性蛋白质相互作用谱揭示BR和SR类受体激酶信号转导的精细调控机制
Lin Xi,Xuna Wu,Jiahui Wang et al.
Lin Xi et al.
At the plasma membrane, in response to biotic and abiotic cues, specific ligands initiate the formation of receptor kinase heterodimers, which regulate the activities of plasma membrane proteins and initiate signaling cascades to the nucleu...
Xiang Zhang,Juan Ge,Yue Wang et al.
Xiang Zhang et al.
Well-controlled metabolism is associated with high-quality oocytes and optimal development of a healthy embryo. However, the metabolic framework that controls mammalian oocyte growth remains unknown. In the present study, we comprehensively...
From Fringe to the Mainstream: How ETD MS Brought O-GlcNAc to the Masses [0.03%]
从边缘到主流：ETD MS技术如何使O-GlcNAc走向大众化的研究之路
Namrata D Udeshi,Gerald W Hart,Chad Slawson
Namrata D Udeshi
O-GlcNAcylation was identified in the 1980s by Torres and Hart and modifies thousands of cellular proteins, yet the regulatory role of O-GlcNAc is still poorly understood compared to the abundance of mechanistic information known for other ...
Proteomic Characterization of 1000 Human and Murine Neutrophils Freshly Isolated From Blood and Sites of Sterile Inflammation [0.03%]
千个来自血液和无菌炎症部位的人类和小鼠中性粒细胞的蛋白质组表征
Susmita Ghosh,Ali Ata Tuz,Martin Stenzel et al.
Susmita Ghosh et al.
Neutrophils are indispensable for defense against pathogens. Injured tissue-infiltrated neutrophils can establish a niche of chronic inflammation and promote degeneration. Studies investigated transcriptome of single-infiltrated neutrophils...
Proteomic Characterization of Ubiquitin Carboxyl-Terminal Hydrolase 19 Deficient Cells Reveals a Role for USP19 in the Secretion of Lysosomal Proteins [0.03%]
泛素羧基末端水解酶19缺陷细胞的蛋白质组特征表明USP19在溶酶体蛋白分泌中的作用
Simone Bonelli,Margot Lo Pinto,Yihong Ye et al.
Simone Bonelli et al.
Ubiquitin carboxyl-terminal hydrolase 19 (USP19) is a unique deubiquitinase, characterized by multiple variants generated by alternative splicing. Several variants bear a C-terminal transmembrane domain that anchors them to the endoplasmic ...
Retinal Proteome Profiling of Inherited Retinal Degeneration Across Three Different Mouse Models Suggests Common Drug Targets in Retinitis Pigmentosa [0.03%]
遗传性视网膜变性的三种小鼠模型的视网膜蛋白质组特征分析提示色素性视网膜炎具有共同的药物靶点
Ahmed B Montaser,Fangyuan Gao,Danielle Peters et al.
Ahmed B Montaser et al.
Inherited retinal degenerations (IRDs) are a leading cause of blindness among the population of young people in the developed world. Approximately half of IRDs initially manifest as gradual loss of night vision and visual fields, characteri...
Profiling Cullin4-E3 Ligases Interactomes and Their Rewiring in Influenza A Virus Infection [0.03%]
流感病毒感染中的Cul4 E3连接酶及其互作蛋白网络的重构
Guillaume Dugied,Thibaut Douche,Melanie Dos Santos et al.
Guillaume Dugied et al.
Understanding the integrated regulation of cellular processes during viral infection is crucial for developing host-targeted approaches. We have previously reported that an optimal in vitro infection by influenza A virus (IAV) requires thre...
Hydrogen/Deuterium Exchange Mass Spectrometry: Fundamentals, Limitations, and Opportunities [0.03%]
氢氘交换质谱：基础、局限性和机遇
Lars Konermann,Pablo M Scrosati
Lars Konermann
Hydrogen/deuterium exchange mass spectrometry (HDX-MS) probes dynamic motions of proteins by monitoring the kinetics of backbone amide deuteration. Dynamic regions exhibit rapid HDX, while rigid segments are more protected. Current data rea...
Multilevel Proteomics Reveals Epigenetic Signatures in BCG-Mediated Macrophage Activation [0.03%]
多级蛋白质组学揭示了BCG介导的巨噬细胞活化中的表观遗传特征
Zoe Schaefer,John Iradukunda,Evelyn N Lumngwena et al.
Zoe Schaefer et al.
The bacillus Calmette-Guérin BCG vaccine (Mycobacterium bovis) is primarily used to prevent tuberculosis (TB) infections but has wide-ranging immunogenic effects. One of its most notable properties is its ability to induce trained immunity...
Super Enhanced Purification of Denatured-Refolded Ubiquitinated Proteins by ThUBD Revealed Ubiquitinome Dysfunction in Liver Fibrosis [0.03%]
利用ThUBD技术分析肝纤维化中泛素蛋白组的异常
Xinyu Cheng,Yonghong Wang,Jinfang Liu et al.
Xinyu Cheng et al.
Ubiquitination is crucial for maintaining protein homeostasis and plays a vital role in diverse biological processes. Ubiquitinome profiling and quantification are of great scientific significance. Artificial ubiquitin-binding domains (UBDs...