Single-Cell Multi-Omics Analysis of In Vitro Post-Ovulatory-Aged Oocytes Revealed Aging-Dependent Protein Degradation [0.03%]
单细胞多组学分析揭示玻璃化老化卵母细胞蛋白质依赖性降解
Yueshuai Guo,Mengmeng Gao,Xiaofei Liu et al.
Yueshuai Guo et al.
Once ovulated, the oocyte has to be fertilized in a short time window or it will undergo post-ovulation aging (POA), whose underlying mechanisms are still not elucidated. Here, we optimized single-cell proteomics methods and performed singl...
Integrative Multi-PTM Proteomics Reveals Dynamic Global, Redox, Phosphorylation, and Acetylation Regulation in Cytokine-Treated Pancreatic Beta Cells [0.03%]
整合多 PTM 组学揭示细胞因子处理的胰岛β细胞中的动态全局、氧化还原、磷酸化和乙酰化调节机制
Austin Gluth,Xiaolu Li,Marina A Gritsenko et al.
Austin Gluth et al.
Studying regulation of protein function at a systems level necessitates an understanding of the interplay among diverse posttranslational modifications (PTMs). A variety of proteomics sample processing workflows are currently used to study ...
In-depth analysis of miRNA binding sites reveals the complex response of uterine epithelium to miR-26a-5p and miR-125b-5p during early pregnancy [0.03%]
对miR-26a-5p和miR-125b-5p在早期妊娠中对子宫上皮的作用的深入分析及miRNA结合位点的复杂响应机制研究
Kamil Myszczynski,Joanna Szuszkiewicz,Kamil Krawczynski et al.
Kamil Myszczynski et al.
Post-transcriptional regulation of gene expression by miRNAs likely makes significant contributions to mRNA abundance at the embryo-maternal interface. In this study, we investigated how miR-26a-5p and miR-125b-5p contribute to molecular ch...
Gradient-Elution Nanoflow Liquid Chromatography Without a Binary Pump: Smoothed Step Gradients Enable Reproducible, Sensitive, and Low-Cost Separations for Single-Cell Proteomics [0.03%]
无二元泵的梯度洗脱纳升液相色谱法：平滑的阶梯型梯度实现了单细胞蛋白质组学分离的可重现性、灵敏性和低成本属性
Kei G I Webber,Siqi Huang,Hsien-Jung L Lin et al.
Kei G I Webber et al.
Mass spectrometry-based proteome profiling of trace analytes including single cells benefits from liquid chromatography separations operated at low flow rates (e.g.,
Bridging the Gap From Proteomics Technology to Clinical Application: Highlights From the 68th Benzon Foundation Symposium [0.03%]
从蛋白质组技术到临床应用：第六届本松恩基金会 symposium 研讨会报告摘要
Vincent Albrecht,Johannes Müller-Reif,Thierry M Nordmann et al.
Vincent Albrecht et al.
The 68th Benzon Foundation Symposium brought together leading experts to explore the integration of mass spectrometry-based proteomics and artificial intelligence to revolutionize personalized medicine. This report highlights key discussion...
Knockdown Proteomics Reveals USP7 as a Regulator of Cell-Cell Adhesion in Colorectal Cancer via AJUBA [0.03%]
蛋白酶体敲低蛋白质组学发现通过AJUBA介导的细胞间粘附作用的去泛素化酶USP7在结直肠癌中的调节功能
Ahood Al-Eidan,Ben Draper,Siyuan Wang et al.
Ahood Al-Eidan et al.
Ubiquitin-specific protease 7 (USP7) is implicated in many cancers including colorectal cancer in which it regulates cellular pathways such as Wnt signaling and the P53-MDM2 pathway. With the discovery of small-molecule inhibitors, USP7 has...
Top-Down Proteomics Identifies Plasma Proteoform Signatures of Liver Cirrhosis Progression [0.03%]
基于质谱的蛋白质组学鉴定出肝硬化发展过程中的血浆蛋白形式标志物
Eleonora Forte,Jes M Sanders,Indira Pla et al.
Eleonora Forte et al.
Cirrhosis, advanced liver disease, affects 2 to 5 million Americans. While most patients have compensated cirrhosis and may be fairly asymptomatic, many decompensate and experience life-threatening complications such as gastrointestinal ble...
The Hunt Lab Guide to De Novo Peptide Sequence Analysis by Tandem Mass Spectrometry [0.03%]
从头解析肽序列的串联质谱分析指南
Lissa C Anderson,Dina L Bai,Greg T Blakney et al.
Lissa C Anderson et al.
Donald Hunt has made seminal contributions to the fields of proteomics, immunology, epigenetics, and glycobiology. The foundation of every important work to come out of the Hunt Laboratory is de novo peptide sequencing. For decades, he taug...
P Jane Gale,George C Stafford,Howard R Morris et al.
P Jane Gale et al.
Arriving at the University of Virginia in the autumn of 1969, Donald Hunt began his 50+ year career in academics with the study of organometallic chemistry, on which he had done his PhD thesis work, and mass spectrometry, to which he was in...
Screening of Cancer-Specific Biomarkers for Hepatitis B-Related Hepatocellular Carcinoma Based on a Proteome Microarray [0.03%]
基于蛋白质微阵列的肝炎B病毒相关肝细胞癌特异性生物标志物筛选
Wudi Hao,Danyang Zhao,Yuan Meng et al.
Wudi Hao et al.
Hepatocellular carcinoma (HCC) is associated with one of the highest mortality rates among cancers, rendering its early diagnosis clinically invaluable. Serum biomarkers, specifically alpha-fetoprotein (AFP), represent the most promising an...