Development of a high-throughput platform for quantitation of histone modifications on a new QTOF instrument [0.03%]
新型QTOF仪器上表观遗传定量高通量平台的建立和发展
Emily Zahn,Yixuan Xie,Xingyu Liu et al.
Emily Zahn et al.
Histone post-translational modifications (PTMs) regulate gene expression patterns through epigenetic mechanisms. The 5 histone proteins (H1, H2A, H2B, H3, and H4) are extensively modified, with over 75 distinct modification types spanning m...
CySP3-96 enables scalable, streamlined, and low-cost sample preparation for cysteine chemoproteomic applications [0.03%]
CySP3-96能够实现半胱氨酸化学蛋白质组学应用的大规模、简便和低成本样品制备
Flowreen Shikwana,Beeta S Heydari,Samuel Ofori et al.
Flowreen Shikwana et al.
Cysteine chemoproteomic screening platforms are widely utilized for chemical probe and drug discovery campaigns. Chemoproteomic compound screens, which use a mass spectrometry-based proteomic readout, can interrogate the structure activity ...
Rensong Ji,Riccardo Zenezini Chiozzi,Henk van den Toorn et al.
Rensong Ji et al.
Sperm cells are terminally differentiated cells that are essential for reproduction in sexually reproducing species. Consistent with their highly specialized function, sperm cells harbor a unique proteome containing many proteins not expres...
Electrophoresis-Correlative Ion Mobility Deepens Single-cell Proteomics in Capillary Electrophoresis Mass Spectrometry [0.03%]
毛细管电泳质谱中电泳-相关离子淌度深化单细胞蛋白质组学研究
Bowen Shen,Fei Zhou,Peter Nemes
Bowen Shen
Detection of trace-sensitive signals is a current challenge in single-cell mass spectrometry (MS) proteomics. Separation prior to detection improves the fidelity and depth of proteome identification and quantification. We recently recognize...
Heterogeneity Assessment and Protein Pathway Prediction via Spatial Lipidomic and Proteomic Correlation: Advancing Dry Proteomics concept for Human Glioblastoma Prognosis [0.03%]
基于空间脂质组学和蛋白质组学关联的异质性评估及蛋白通路预测：推进干式蛋白质组学概念以改善人类胶质母细胞瘤预后
Laurine Lagache,Yanis Zirem,Émilie Le Rhun et al.
Laurine Lagache et al.
Prediction of proteins and associated biological pathways from lipid analyses via MALDI MSI is a pressing challenge. We introduced "dry proteomics," using MALDI MSI to validate spatial localization of identified optimal clusters in lipid im...
Nuclear Factor I family members are key transcription factors regulating gene expression [0.03%]
核因子I家族成员是调节基因表达的关键转录因子
Dicle Malaymar Pinar,Helka Göös,Zenglai Tan et al.
Dicle Malaymar Pinar et al.
The Nuclear Factor I (NFI) family of transcription factors (TFs) plays key roles in cellular differentiation, proliferation, and homeostasis. As such, NFI family members engage in large number of interactions with other proteins and the chr...
Identification of Critical Phosphorylation Sites Enhancing Kinase Activity with a Bimodal Fusion Framework [0.03%]
利用双模态融合框架识别增强激酶活性的关键磷酸化位点
Menghuan Zhang,Yizhi Zhang,Keqin Dong et al.
Menghuan Zhang et al.
Phosphorylation is an indispensable regulatory mechanism in cells, with specific sites on kinases that can significantly enhance their activity. Although several such critical phosphorylation sites (phos-sites) have been experimentally iden...
Global proteomics indicates subcellular-specific anti-ferroptotic responses to ionizing radiation [0.03%]
全球蛋白质组学表明细胞器特异性抗铁死亡反应对抗离子辐射
Josie A Christopher,Lisa M Breckels,Oliver M Crook et al.
Josie A Christopher et al.
Cells have many protective mechanisms against background levels of ionizing radiation (IR) orchestrated by molecular changes in expression, post-translational modifications and subcellular localization. Radiotherapeutic treatment in oncolog...
Shaowen Wu,Sheng Zhang,Chun-Ming Liu et al.
Shaowen Wu et al.
The foundation of all biological processes is the network of diverse and dynamic protein interactions with other molecules in cells known as the interactome. Understanding the interactome is crucial for elucidating molecular mechanisms but ...
Chemical glycoproteomic profiling in rice seedlings reveals N-glycosylation in the ERAD-L machinery [0.03%]
化学糖蛋白谱分析揭示了水稻幼苗中内质网相关蛋白质降解-脂致死结构元件中的N-糖基化现象
Cong Lei,Xilong Li,Wenjia Li et al.
Cong Lei et al.
As a ubiquitous and essential posttranslational modification occurring in both plants and animals, protein N-linked glycosylation regulates various important biological processes. Unlike the well-studied animal N-glycoproteomes, the landsca...