Comprehensive proteomics metadata and integrative web portals facilitate sharing and integration of LINCS multiomics data [0.03%]
综合蛋白质组学元数据和整合网络端口促进共享和整合LINCS多组学数据
Dušica Vidović,Behrouz Shamsaei,Stephan C Schürer et al.
Dušica Vidović et al.
The Library of Integrated Network-based Cellular Signatures (LINCS), an NIH Common Fund program, has cataloged and analyzed cellular function and molecular activity profiles in response to >80,000 perturbing agents that are potentially disr...
Sofia Ainatzi,Svenja V Kaufmann,Ivan Silbern et al.
Sofia Ainatzi et al.
Neuronal communication relies on neurotransmitter release from synaptic vesicles (SVs), whose dynamics are controlled by Ca2+-dependent pathways, as many thoroughly studied phosphorylation cascades. However, little is known about other post...
Addressing NHS chemistry: Efficient quenching of excess TMT reagent and reversing TMT-over labelling in proteomic samples by methylamine [0.03%]
应对NHS化学在蛋白质组样品中的高效淬灭过量的TMT试剂和通过甲胺逆转TMT过度标记
Yana Demyanenko,Xintong Sui,Andrew M Giltrap et al.
Yana Demyanenko et al.
N-Hydroxysuccinimide (NHS) ester chemistry is used extensively across proteomics sample preparation. One of its increasingly prevalent applications is in isobaric reagent-based quantitation such as iTRAQ (isobaric tags for relative and abso...
Benchmarking of quantitative proteomics workflows for Limited proteolysis mass spectrometry [0.03%]
有限蛋白水解质谱定量蛋白质组学工作流程的基准测试
Tomas Koudelka,Claudio Bassot,Ilaria Piazza
Tomas Koudelka
Limited proteolysis coupled with mass spectrometry (LiP-MS) has emerged as a powerful technique for detecting protein structural changes and drug-protein interactions on a proteome-wide scale. However, there is no consensus on the best quan...
Proteomics analysis of porcine endometrial cell-derived extracellular vesicles involved in embryo attachment [0.03%]
猪子宫内膜细胞来源的胚胎附植相关胞外囊泡的蛋白质组学分析
Seonggyu Bang,Ahmad Yar Qamar,Sang-Yeop Lee et al.
Seonggyu Bang et al.
Maternal-embryo interactions play a critical role in early mammalian development, with extracellular vesicles (EVs) playing a key role in intercellular communication. Recent studies have focused on the mechanisms by which maternal-derived f...
The Hunt lab weighs in on mass spectrometry-based analysis of protein post-translational modifications [0.03%]
Hunt实验室对基于质谱的蛋白质翻译后修饰分析发表见解
Joshua J Coon,Jarrod A Marto,John E P Syka et al.
Joshua J Coon et al.
Protein post-translational modifications have traditionally been challenging to identify due to their dynamic regulation and typically low stoichiometry. Methods for phosphopeptide enrichment from complex proteomes developed in the Hunt lab...
L Nagel,J Grossbach,V Cappelletti et al.
L Nagel et al.
Limited proteolysis combined with mass spectrometry (LiP-MS) facilitates probing structural changes on a proteome-wide scale. This method leverages differences in the proteinase K accessibility of native protein structures to concurrently a...
Upregulation of protein O-GlcNAcylation levels promotes zebrafish fin regeneration [0.03%]
蛋白质O-连接N-乙酰葡萄糖苷修饰水平的上调促进斑马鱼鳍再生
Liyuan Jia,Hanxue Zheng,Juantao Feng et al.
Liyuan Jia et al.
As one of the most important post-translational modifications, glycosylation participates in various cellular activities in organisms and is closely associated with many pathogeneses. It has been reported that glycosylation affects liver, s...
PEPSeek-mediated identification of novel epitopes from viral and bacterial pathogens and the impact on host cell immunopeptidomes [0.03%]
PEPSeek介导的病毒和细菌病原体新型表位的鉴定及其对宿主细胞免疫肽组的影响
John A Cormican,Lobna Medfai,Magdalena Wawrzyniuk et al.
John A Cormican et al.
Here, we develop PEPSeek, a web-server based software to allow higher performance in the identification of pathogen-derived epitope candidates detected via mass spectrometry in MHC class I immunopeptidomes. We apply it to human and mouse ce...
diaPASEF analysis for HLA-I peptides enables quantification of common cancer neoantigens [0.03%]
diaPASEF分析HLA-I肽可实现常见癌症新抗原的定量
Denys Oliinyk,Hem R Gurung,Zhenru Zhou et al.
Denys Oliinyk et al.
Human leukocyte antigen class I (HLA-I) molecules present short peptide sequences from endogenous or foreign proteins to cytotoxic T cells. The low abundance of HLA-I peptides poses significant technical challenges for their identification ...