An improved method for sampling and quantitative protein analytics of cerebrospinal fluid of individual mice [0.03%]
改进的采样及量化分析单个小鼠脑脊液蛋白质的方法
Athanasios Lourbopoulos,Stephan A Müller,Georg Jocher et al.
Athanasios Lourbopoulos et al.
The mouse is the species most commonly used in preclinical research, but protein analytics of murine cerebrospinal fluid (CSF) remains challenging because of low sample volumes (often
Metal ion-enhanced ZIC-cHILIC StageTip for N-Glycoproteomic and Phosphoproteomic Profiling in EGFR-mutated Lung Cancer Cells [0.03%]
金属离子增强的ZIC-cHILIC StageTip用于EGFR突变肺癌细胞的N-糖蛋白质组和磷酸蛋白质组谱分析
Yi-Ju Chen,Yan-Lin Chen,Kun-Hao Chang et al.
Yi-Ju Chen et al.
Surface glycosylation and intracellular phosphorylation regulates the cell-cell communication and signaling cascades. Due to complex glycosylation and dynamic phosphorylation, exploring their interplay remains technically challenging. In th...
Bioinformatics-Guided Identification and Quantification of Biomarkers of Crotalus atrox Envenoming and its Neutralization by Antivenom [0.03%]
毒液生物标志物的生物信息学指导识别与量化及其抗蛇毒血清中和效应分析
Auwal A Bala,Anas Bedraoui,Salim El Mejjad et al.
Auwal A Bala et al.
Quantitative mass spectrometry-based proteomics of extracellular vesicles (EVs) provides systems-level exploration for the analysis of snakebite envenoming (SBE) as the venom progresses, causing injuries such as hemorrhage, trauma, and deat...
Proteomic Analysis of Aqueous Humor Identified Clinically Relevant Molecular Targets for Neovascular Complications in Diabetic Retinopathy [0.03%]
玻璃体蛋白组学分析确定了糖尿病视网膜病变血管并发症的临床相关分子靶标
Jae Won Oh,Seong Joon Ahn,Jae Hun Jung et al.
Jae Won Oh et al.
Diabetic retinopathy (DR) is a leading cause of blindness in adults under 40 in the developed world, with a significant proportion progressing to vision-threatening stages such as proliferative diabetic retinopathy (PDR) and neovascular gla...
Proteomics and machine learning-based approach to decipher subcellular proteome of mouse heart [0.03%]
基于蛋白质组学和机器学习的策略破译小鼠心脏亚细胞蛋白组结构
Haoyun Fang,Alin Rai,Seyed Sadegh Eslami et al.
Haoyun Fang et al.
Protein compartmentalisation to distinctive subcellular niches is critical for cardiac function and homeostasis. Here, we employed a rapid and robust workflow based on differential centrifugal-based fractionation with mass spectrometry (MS)...
Deleterious knock-outs in the HLA class I antigen processing and presentation machinery induce distinct changes in the immunopeptidome [0.03%]
HLA I类抗原处理和呈现机器中的有害基因敲除引起免疫肽组的显著变化
Ilja E Shapiro,Clélia Maschke,Justine Michaux et al.
Ilja E Shapiro et al.
The human leukocyte antigen (HLA) processing and presentation machinery (APPM) is altered in various diseases and in response to drug treatments. Defects in the machinery may change presentation levels or alter the repertoire of presented p...
A Single-Step Protein Extraction for Lung Extracellular Matrix Proteomics Enabled by the Photocleavable Surfactant Azo and timsTOF Pro [0.03%]
光裂解表面活性剂Azo和timtTOF Pro实现的单步肺细胞外基质蛋白质组学蛋白质提取方法
Anna G Towler,Andrew J Perciaccante,Timothy J Aballo et al.
Anna G Towler et al.
The extracellular matrix (ECM) is a dynamic, complex network of proteins, collectively known as the 'matrisome', which not only provides essential structural support to cells and tissues but also regulates critical cellular processes. Dysre...
Single-Cell Proteomic Characterization of Drug-Resistant Prostate Cancer Cells Reveals Molecular Signatures Associated with Morphological Changes [0.03%]
单细胞蛋白质特征分析揭示药物抗性前列腺癌细胞的形态变化相关分子标记
Jongmin Woo,Michael Loycano,Md Amanullah et al.
Jongmin Woo et al.
This study delves into the proteomic intricacies of drug-resistant cells (DRCs) within prostate cancer, which are known for their pivotal roles in therapeutic resistance, relapse, and metastasis. Utilizing single-cell proteomics (SCP) with ...
Metabolic Reprogramming into a Glycolysis Phenotype Induced by Extracellular Vesicles Derived from Prostate Cancer Cells [0.03%]
前列腺癌细胞来源的胞外囊泡诱导的代谢重编程进入糖酵解表型
Yoon-Jin Lee,Chul Won Seo,Shinwon Chae et al.
Yoon-Jin Lee et al.
Most cancer cells adopt a less efficient metabolic process of aerobic glycolysis with high level of glucose uptake followed by lactic acid production, known as the Warburg effect. This phenotypic transition enables cancer cells to achieve i...
Comprehensive proteomics metadata and integrative web portals facilitate sharing and integration of LINCS multiomics data [0.03%]
综合蛋白质组学元数据和整合网络端口促进共享和整合LINCS多组学数据
Dušica Vidović,Behrouz Shamsaei,Stephan C Schürer et al.
Dušica Vidović et al.
The Library of Integrated Network-based Cellular Signatures (LINCS), an NIH Common Fund program, has cataloged and analyzed cellular function and molecular activity profiles in response to >80,000 perturbing agents that are potentially disr...