Limited impact of column chemistry and length on proteome coverage under high-speed DIA [0.03%]
高速DIA条件下反相色谱柱填料化学组成及长度对蛋白质组覆盖的有限影响
Alicia-Sophie Schebesta,Kathrin Korff,Ericka C M Itang et al.
Alicia-Sophie Schebesta et al.
The evolution of mass spectrometry (MS)-based proteomics has been driven by continuous technological advances in sample preparation, liquid-phase separations, instrumentation, and data acquisition. Chromatographic performance has been recog...
A Parallel Accumulation-Mobility Aligned Fragmentation Strategy Utilizing High-Resolution Ion Mobility for High Performance Proteomics Analysis [0.03%]
一种利用高分辨率离子淌度的并行累积-动力学对齐碎裂策略，用于高性能蛋白质组分析
Leonard C Rorrer rd,Liulin Deng,Lauren Royer et al.
Leonard C Rorrer rd et al.
Here we present a novel data independent acquisition (DIA) mass spectrometry (MS) operating mode termed parallel accumulation-mobility aligned fragmentation (PAMAF) that offers enhanced speed and sensitivity of ion fragmentation analysis fo...
Optimizing NGN2 Dosage Enhances the Neuronal Enrichment of iPSC-derived Neuronal Cultures [0.03%]
优化NGN2剂量可增强iPSC来源的神经元培养物中的神经元富集度
Jesús Muñoz-Estrada,Andrew Mostafania,Lahiruni Halwatura et al.
Jesús Muñoz-Estrada et al.
Proteomic analysis of highly neuron-enriched iPSC-derived cultures can provide valuable insights into the pathogenesis of neurological diseaseby minimizing confounding signals from non-neuronal populations . However, single-cell iPSC clones...
DORSSAA: Drug-target interactOmics Resource based on Stability/Solubility Alteration Assay [0.03%]
基于稳定性和溶解度改变测定的药物靶点互作组学资源(DORSSAA)
Ehsan Zangene,Elham Gholizadeh,Veit Schwämmle et al.
Ehsan Zangene et al.
Advancements in high-throughput techniques such as Thermal Proteome Profiling (TPP) and the high-throughput Proteome Integral Solubility Alteration (PISA) assay have revolutionized our understanding of drug-protein interactions. Despite the...
Proteome-wide ubiquitinome profiling reveals substrate-specific dynamics within the USP7 network [0.03%]
泛素蛋白质组分析揭示了USP7网络中的底物特异性动力学特征
Joyce Wolf van der Meer,Jan A van der Knaap,Ayestha Sijm et al.
Joyce Wolf van der Meer et al.
USP7 is a pleiotropic deubiquitylating enzyme that is involved in tumor suppression, (neuro)development, chromatin regulation and the DNA damage response. How USP7 regulates these diverse pathways is still unclear. Here, we report data-inde...
Julie Manessier,Hassan Hijazi,Lisa Vizzini et al.
Julie Manessier et al.
Dynamic histone post-translational modifications are crucial to precisely orchestrate gene expression programs. The recently discovered histone lysine lactylation has already been explored in various pathological contexts, but less in norma...
Long-chain S-acylation is a key modulator during the macrophage inflammatory response [0.03%]
长链S-酰化在巨噬细胞炎性反应中起关键调节作用
Anneroos E Nederstigt,Samiksha Sardana,Francine R Ianiski et al.
Anneroos E Nederstigt et al.
Long-chain S-acylation is a reversible lipid modification critical for regulating protein localization, stability, and signaling, yet its role in macrophage-mediated inflammation remains incompletely understood. Here, we combine stable isot...
LCAT acts as a molecular switch and regulates rooster sperm motility in vitro [0.03%]
LCAT充当分子开关调节公鸡精子体外的运动能力
Jiwei Li,Yuzhu Cao,Juan Zhang et al.
Jiwei Li et al.
Sperm motility is a critical determinant of semen quality, yet the molecular mechanisms regulating motility remain unclear. In this study, we identified Yufen 1 roosters with significant differences in sperm motility among highly motile spe...
dia-PASEF Enables Rapid Profiling of the Human Secretome for Deeper Insights into Cellular Dynamics and Inflammatory Mechanisms [0.03%]
dia-PASEF促进人类分泌组的快速剖析以更深入地了解细胞动力学和炎症机制
Chloe L Tayler,Serena Bateman,Charlie Haslam et al.
Chloe L Tayler et al.
Protein secretion is a fundamental mechanism for cellular coordination and signalling, with its dysregulation leading to widespread physiological dysfunction and disease. Immunoassay formats that utilise secondary antibody readouts are the ...
π-SeqOmics: A Sequential Workflow for Genomic, Transcriptomic, Proteomic and Phosphoproteomic Profiling from Biopsy-Scale Samples [0.03%]
π-SeqOmics：一种用于基因组、转录组、蛋白质组和磷酸化蛋白质组生物检材样本分析的流程式工作流方法
Shuyi Feng,Xuehui Deng,Ying Xu et al.
Shuyi Feng et al.
Comprehensive multi-omics profiling from a single limited specimen remains challenging, as current extraction methods are difficult to standardize and often compromise balanced recovery of nucleic acids and proteins. Here, we developed π-S...