Photonic Crystal Surfaces as a General Purpose Platform for Label-Free and Fluorescent Assays [0.03%]
光子晶体表面作为通用的无标签和荧光检测平台
Brian T Cunningham
Brian T Cunningham
Photonic crystal surfaces can be designed to provide a wide range of functions that are used to perform biochemical and cell-based assays. Detection of the optical resonant reflections from photonic crystal surfaces enables high sensitivity...
Automated high-throughput microchannel assays for cell biology: Operational optimization and characterization [0.03%]
用于细胞生物学的自动化高通量微通道检测：运行优化与表征
John P Puccinelli,Xiaojing Su,David J Beebe
John P Puccinelli
Screening biological readouts in cell culture are increasing in frequency and throughput. In such assays, cell types may be rare and reagents or compounds may be expensive often resulting in a reduced number of conditions and/or replicates....
Walson Lawi,Chris Wiita,Steven T Snyder et al.
Walson Lawi et al.
Cartridge-based microfluidics is a promising technology for clinical diagnostics. By miniaturizing the fluid-handling processes required for genomic and proteomic analyses, reagent and specimen volume is minimized along with the size of the...
Analysis of Retina and Erythrocyte Glycerophospholipid Alterations in a Rat Model of Type 1 Diabetes [0.03%]
1型糖尿病大鼠模型视网膜和红细胞甘油磷脂变化分析
Todd A Lydic,Rebecca Renis,Julia V Busik et al.
Todd A Lydic et al.
An automated tandem mass spectrometry based analysis employing precursor ion and neutral loss scans in a triple quadrupole mass spectrometer has been employed to identify and quantify changes in the abundances of glycerophospholipids extrac...
Perspectives on utilizing unique features of microfluidics technology for particle and cell sorting [0.03%]
基于微流控技术特点的颗粒及细胞分选研究进展
Jonathan D Adams,H Tom Soh
Jonathan D Adams
Sample preparation is often the most tedious and demanding step in an assay, but it also plays an essential role in determining the quality of results. As biological questions and analytical methods become increasingly sophisticated, there ...
The Stanford Automated Mounter: Enabling High-Throughput Protein Crystal Screening at SSRL [0.03%]
斯坦福大学自动安装器：在SSRL进行高通量蛋白质晶体筛选的功能
Clyde A Smith,Aina E Cohen
Clyde A Smith
The macromolecular crystallography experiment lends itself perfectly to high-throughput technologies. The initial steps including the expression, purification and crystallization of protein crystals, along with some of the later steps invol...
Adapting Cell-Based Assays to the High Throughput Screening Platform: Problems Encountered and Lessons Learned [0.03%]
细胞实验在高通量筛选平台的应用：遇到的问题及经验教训
Clinton B Maddox,Lynn Rasmussen,E Lucile White
Clinton B Maddox
In recent years, cell-based phenotypic assays have emerged as an effective and robust addition to the array of assay technologies available for drug discovery in the high throughput screening arena. Previously, biochemical target-based assa...
Coupling Microdialysis Sampling to Microchip Electrophoresis in a Reversibly Sealed Device [0.03%]
通过可逆密封器件将微透析取样与芯片电泳联用
Laura C Mecker,R Scott Martin
Laura C Mecker
In this paper, we describe the fabrication and characterization of a reversibly sealed microchip device that is used to couple microdialysis sampling to microchip electrophoresis. The ability to interface microdialysis sampling and microchi...
Adam Yasgar,Paul Shinn,Ajit Jadhav et al.
Adam Yasgar et al.
An efficient and versatile Compound Management operation is essential for the success of all downstream processes in high-throughput screening (HTS) and small molecule lead development. Staff, equipment, and processes need to be not only re...
An instrument for automated purification of nucleic acids from contaminated forensic samples [0.03%]
一种从污染的法医样本中自动提取核酸的仪器
David J Broemeling,Joel Pel,Dylan C Gunn et al.
David J Broemeling et al.
Forensic crime scene sample analysis, by its nature, often deals with samples in which there are low amounts of nucleic acids, on substrates that often lead to inhibition of subsequent enzymatic reactions such as PCR amplification for STR p...