Afp::mCherry, a red fluorescent transgenic reporter of the mouse visceral endoderm [0.03%]
Afp::mCherry小鼠胚胎视觉报告基因线路构建及应用
Manuel Viotti,Sonja Nowotschin,Anna-Katerina Hadjantonakis
Manuel Viotti
Live imaging of genetically encoded fluorescent protein reporters is increasingly being used to investigate details of the cellular behaviors that underlie the large-scale tissue rearrangements that shape the embryo. However, the majority o...
Use of fully modified 2'-O-methyl antisense oligos for loss-of-function studies in vertebrate embryos [0.03%]
全修饰的2'-O-甲基反义寡核苷酸在脊椎动物胚胎功能缺失研究中的应用
Patricia N Schneider,John T Olthoff,Abby J Matthews et al.
Patricia N Schneider et al.
Antisense oligonucleotides are commonly employed to study the roles of genes in development. Although morpholino phosphorodiamidate oligonucleotides (morpholinos) are widely used to block translation or splicing of target gene products' the...
Development of transgenic mice expressing a conditionally active form of the eIF2α kinase PKR [0.03%]
条件性活化的eIF2α激酶PKR转基因小鼠的建立
Andreas I Papadakis,Dionissios Baltzis,Ryan C Buensuceso et al.
Andreas I Papadakis et al.
Phosphorylation of the alpha (α) subunit of the eukaryotic initiation factor 2 (eIF2) at serine 51 is an important mechanism of translational control in response to various forms of environmental stress. In metazoans, eIF2α phosphorylatio...
A tryptophan hydroxlyase 1 reporter that directs Cre recombinase extinguishable placental alkaline phosphatase expression in serotonergic (5-HT) neurons and peripheral tissues [0.03%]
一种血清素神经元和外周组织中的色氨酸羟化酶1报告工具，可在指定位置重新组合生成克雷重组酶灭活的胎盘碱性磷酸酶表达
Michael L Huynh,Elena Rivkin,Ryan Mui et al.
Michael L Huynh et al.
The serotonergic (5-HT) system modulates many behaviors and has been implicated in psychiatric disorders, but the density of 5-HT processes has complicated analyses. We have used regulatory regions from the Tryptophan hydroxylase 1 (Tph1) g...
Efficient inducible Cre-mediated recombination in Tcf21 cell lineages in the heart and kidney [0.03%]
心脏和肾脏中Tcf21细胞谱系的高效诱导型Cre介导重组
Asha Acharya,Seung Tae Baek,Serena Banfi et al.
Asha Acharya et al.
Tcf21 is a Class II bHLH family member with essential roles in the formation of the lungs, kidneys, gonads, spleen, and heart. Here, we report the utility of a mouse line with targeted insertion of a tamoxifen-inducible Cre recombinase, Mer...
Generation of mice with a conditional allele for the p75(NTR) neurotrophin receptor gene [0.03%]
制备条件性失活p75（NTR）神经营养因子受体基因的小鼠品系
Emil Bogenmann,Penny S Thomas,Qianfeng Li et al.
Emil Bogenmann et al.
The p75(NTR) neurotrophin receptor has been implicated in multiple biological and pathological processes. While significant advances have recently been made in understanding the physiologic role of p75(NTR) , many details and aspects remain...
Heads-up: new roles for the fragile X mental retardation protein in neural stem and progenitor cells [0.03%]
顶体提示：脆性X综合征智力低下蛋白在神经干细胞和前体细胞中的新作用
Matthew A Callan,Daniela C Zarnescu
Matthew A Callan
Fragile X syndrome (FXS) is the most common form of inherited mental retardation and is caused by the loss of function for Fragile X Mental Retardation Protein (FMRP), a selective RNA-binding protein with a demonstrated role in the localize...
Jing Cai,Liang Ma
Jing Cai
Epithelial-mesenchymal interactions underlie the foundation for ectodermal appendage formation. Signal molecules such as BMPs and WNTs mediate crosstalk between the two tissue layers and coordinate both the induction and morphogenesis of ec...
A new allele of Lyl1 confirms its important role in hematopoietic stem cell function [0.03%]
一个新的Lyl1等位基因确认了它在造血干细胞功能中的重要作用
George P Souroullas,Margaret A Goodell
George P Souroullas
Lyl1 codes for a bHLH protein that is an important regulator of hematopoietic stem cell function. An existing mutant allele of Lyl1 features a lacZ gene inserted in-frame into the fourth exon, leaving behind the N-terminus and the DNA-bindi...
Odd-skipped maintains prohemocyte potency and blocks blood cell development in Drosophila [0.03%]
Odd-skipped维持前血细胞潜能并阻止Drosophila的血液细胞发育
Hongjuan Gao,Xiaorong Wu,Nancy Fossett
Hongjuan Gao
Studies using Drosophila have contributed significantly to our understanding of regulatory mechanisms that control stem cell fate choice. The Drosophila blood cell progenitor or prohemocyte shares important characteristics with mammalian he...