Dynamic lineage analysis of embryonic morphogenesis using transgenic quail and 4D multispectral imaging [0.03%]
利用转基因鸡和四维多光谱成像进行胚胎形态发生的谱系动态分析
Danielle V Bower,Yuki Sato,Rusty Lansford
Danielle V Bower
We describe the development of transgenic quail that express various fluorescent proteins in targeted manners and their use as a model system that integrates advanced imaging approaches with conventional and emerging molecular genetics tech...
Toward high-content/high-throughput imaging and analysis of embryonic morphogenesis [0.03%]
朝向胚胎形态发生的高内涵/高通量成像与分析
Thai V Truong,Willy Supatto
Thai V Truong
In vivo study of embryonic morphogenesis tremendously benefits from recent advances in live microscopy and computational analyses. Quantitative and automated investigation of morphogenetic processes opens the field to high-content and high-...
Inducible recombination in the cardiac conduction system of minK: CreERT² BAC transgenic mice [0.03%]
可在minK：CreERT² BAC转基因小鼠心脏传导系统中诱导重组
David E Arnolds,Ivan P Moskowitz
David E Arnolds
Inducible Cre recombination is a powerful technology that allows for spatial and temporal modulation of gene expression in vivo. Diseases of the cardiac conduction system (CCS) pose a significant clinical burden but are not currently well u...
A mouse reporter line to conditionally mark nuclei and cell membranes for in vivo live-imaging [0.03%]
一种用于在体内活体成像中条件性标记细胞核和细胞膜的小鼠报告基因系
Go Shioi,Hiroshi Kiyonari,Takaya Abe et al.
Go Shioi et al.
Live-imaging is an essential tool to visualize live cells and monitor their behaviors during development. This technology demands a variety of mouse reporter lines, each uniquely expressing a fluorescent protein. Here, we developed an R26R-...
An in vivo map of bone morphogenetic protein 2 post-transcriptional repression in the heart [0.03%]
心房中骨形态发生蛋白2转录后抑制的体内图谱
Boudewijn P T Kruithof,Junwang Xu,David T Fritz et al.
Boudewijn P T Kruithof et al.
The Bmp2 3'untranslated region (UTR) sequence bears a sequence conserved between mammals and fishes that can post-transcriptionally activate or repress protein synthesis. We developed a map of embryonic cells in the mouse where this potent ...
Offspring from intracytoplasmic sperm injection of aged mouse oocytes treated with caffeine or MG132 [0.03%]
咖啡因或MG132处理老化卵母细胞后以单精子胞浆内注射产生子代小鼠
Tetsuo Ono,Eiji Mizutani,Chong Li et al.
Tetsuo Ono et al.
Postovulatory mammalian oocytes age significantly in culture. B6D2F1 or ICR strain mouse oocytes were collected 16 h after hCG injection and then cultured for up to 40 h post hCG at 37 °C under 5% CO(2) in air. After intracytoplasmic sperm...
Isolation and live imaging of enteric progenitors based on Sox10-Histone2BVenus transgene expression [0.03%]
基于Sox10-Histone2BVenus转基因表达的肠系粘膜神经干细胞的分离和活体成像研究
Jennifer C Corpening,Karen K Deal,V Ashley Cantrell et al.
Jennifer C Corpening et al.
To facilitate dynamic imaging of neural crest (NC) lineages and discrimination of individual cells in the enteric nervous system (ENS) where close juxtaposition often complicates viewing, we generated a mouse BAC transgenic line that drives...
Peter Farlie,Sally A Moody
Peter Farlie
Yu Lan,Han Liu,Catherine E Ovitt et al.
Yu Lan et al.
The Odd-skipped related 1 (Osr1) gene encodes a zinc finger protein homologous to the Drosophila Odd-skipped transcription factor. During mouse embryogenesis, Osr1 is expressed in multiple tissues, including the developing heart, kidney, li...
Establishment of conditional reporter mouse lines at ROSA26 locus for live cell imaging [0.03%]
在ROSALOCUS处建立用于活细胞成像的条件型报告基因小鼠品系
Takaya Abe,Hiroshi Kiyonari,Go Shioi et al.
Takaya Abe et al.
A series of conditional reporter mouse lines were established in which specific organelles were labeled with fluorescent proteins. Subcellular localization and intensity of 28 fluorescent fusion-protein constructs were surveyed in cell line...