High-performance liquid chromatography coupled with tandem mass spectrometry for the detection of amyloid Beta peptide related with Alzheimer's disease [0.03%]
高效液相色谱-串联质谱检测与阿尔茨海默病相关的淀粉样β肽
Chengan Du,Corinne Ramaley,Hugh McLean et al.
Chengan Du et al.
Recent studies show that quantitative and qualitative differences in amyloid beta (Abeta ) peptides may be implicated in the development of Alzheimer's disease. New evidence seems to support the existence of a dynamic equilibrium between Ab...
Comparison of Golgi apparatus and endoplasmic reticulum proteins from livers of juvenile and aged rats using a novel technique for separation and enrichment of organelles [0.03%]
一种新型细胞器分离和富集技术在肝脏高尔基体和内质网蛋白比较中的应用——以幼年大鼠和老年大鼠为例
Kate L Drahos,Hung-Cuong Tran,Ajay N Kiri et al.
Kate L Drahos et al.
The broad dynamic range of protein abundances, which can vary from about 10(6) for cells to 10(10) for tissues in complex proteomes, continues to challenge proteomics research. Proteome analysis, in particular organelle proteomics, using cu...
Comparative Study
Journal of biomolecular techniques : JBT. 2005 Dec;16(4):347-55. DOI: 2005
David K Crockett,Charles E Seiler rd,Kojo S J Elenitoba-Johnson et al.
David K Crockett et al.
As the reliable identification of proteins by tandem mass spectrometry becomes increasingly common, the full characterization of large data sets of proteins remains a difficult challenge. Our goal was to survey the proteome of a human T-cel...
Combination primer polymerase chain reaction for multi-site mutagenesis of close proximity sites [0.03%]
邻近位点多位点饱和突变的组合引物PCR方法
Pia Hønnerup Jensen,Dietmar Weilguny
Pia Hønnerup Jensen
We describe a rapid and efficient polymerase chain reaction procedure for multi-site-directed mutagenesis for cases in which the sites to be mutated are in close proximity. The combination primer polymer chain reaction method is based on a ...
Optimization of reversed-phase peptide liquid chromatography ultraviolet mass spectrometry analyses using an automated blending methodology [0.03%]
利用自动化混合方法优化反相肽液相色谱紫外质谱分析
Asish B Chakraborty,Scott J Berger
Asish B Chakraborty
The balance between chromatographic performance and mass spectrometric response has been evaluated using an automated series of experiments where separations are produced by the real-time automated blending of water with organic and acidic ...
Identification of Hedysarum varieties using amplified fragment length polymorphism on a capillary electrophoresis system [0.03%]
应用毛细管电泳系统扩增片段长度多态性鉴别黄耆属植物品种
Surekha Karudapuram,Steve Larson
Surekha Karudapuram
For non-model organisms that do not have sequence information readily available, amplified fragment length polymorphism (AFLP) is a well-established technique that can be used for genomic mapping applications such as genetic diversity studi...
A Irina Mutiu,Christopher J Brandl
A Irina Mutiu
RNA isolation from yeast is complicated by the need to initially break the cell wall. While this can be accomplished by glass bead disruption or enzyme treatment, these approaches result in DNA contamination and/or the need for incubation p...
Ingrid D Cruzado-Park,Edna Betgovargez,Chitra Ratnayake et al.
Ingrid D Cruzado-Park et al.
The ultimate goal of proteomics is to understand complex biological systems. The first step toward this end is the discovery of protein differences by profiling a given proteome. One approach to proteome profiling is to fractionate it into ...
Edna Betgovargez,Vita Knudson,Michael H Simonian
Edna Betgovargez
This paper presents a multidimensional profile of the human serum proteome, produced by a two-dimensional protein fractionation system based on liquid chromatography followed by characterization with capillary electrophoresis (CE). The firs...
Vijay G Ramakrishnan,Majd N Aljamali,John R Sauer et al.
Vijay G Ramakrishnan et al.
Ticks are obligate ectoparasites that feed on a variety of hosts including mammals, birds and reptiles. Prolonged attachment on the host and an ability to transmit a wide variety of pathogens are the special features of tick feeding. Saliva...