Sulfonation chemistry as a powerful tool for MALDI TOF/TOF de novo sequencing and post-translational modification analysis [0.03%]
磺化化学作为MALDI TOF/TOF从头测序和翻译后修饰分析的有力工具
Paolo Conrotto,Ulf Hellman
Paolo Conrotto
Mass spectrometry using matrix-assisted laser desorption/ionization (MALDI) is a widespread technique for various types of proteomic analysis. In the identification of proteins using peptide mass fingerprinting, samples are enzymatically di...
MALDI QqTOF MS combined with off-line HPLC for characterization of protein primary structure and post-translational modifications [0.03%]
基质辅助激光解析电离QQTOFMS结合线性离子阱和色谱法表征蛋白质一级结构及翻译后修饰
Oleg V Krokhin,Werner Ens,Kenneth G Standing
Oleg V Krokhin
The addition of off-line high-performance liquid chromatography to matrix-assisted laser desorption/ionization mass spectrometry greatly reduces congestion in the mass spectra, and also provides complete decoupling of the separation process...
Comparative Study
Journal of biomolecular techniques : JBT. 2005 Dec;16(4):429-40. DOI: 2005
Concentration and desalting of peptide and protein samples with a newly developed C18 membrane in a microspin column format [0.03%]
一种新型C18微孔膜在微型离心柱中用于多肽和蛋白质样品的富集及脱盐
Mike J Naldrett,Robert Zeidler,Karen E Wilson et al.
Mike J Naldrett et al.
Protein identification plays an important role in today's academic and industrial proteomic research. Commonly used methods for the separation of proteins from complex samples include liquid chromatography (e.g., ion exchange, reversed-phas...
Reproducibility of retention time using a splitless nanoLC coupled to an ESI-FTICR mass spectrometer [0.03%]
无分流式nanoLC与ESI-FTICR质谱仪联用时的保留时间重现性研究
Christopher J Mason,Kenneth L Johnson,David C Muddiman
Christopher J Mason
Replicate injections of a myoglobin tryptic digest, ultrafiltrates of human serum, and ultrafiltrates of human plasma made on a splitless nanoscale liquid chromatography system coupled to a Fourier-transform ion cyclotron resonance mass spe...
Comparative Study
Journal of biomolecular techniques : JBT. 2005 Dec;16(4):414-22. DOI: 2005
Selective isolation of glycoproteins and glycopeptides for MALDI-TOF MS detection supported by magnetic particles [0.03%]
磁性颗粒辅助的基质辅助激光解析电离飞行时间质谱检测糖蛋白和糖肽的分离富集方法研究
Katrin Sparbier,Sonja Koch,Irina Kessler et al.
Katrin Sparbier et al.
Glycosylation is the most common form of posttranslational modification of proteins (50-80%). The isolation, discovery, and subsequent identification of glycosylated peptides and proteins is becoming more and more important in glycoproteomi...
The SNPlex genotyping system: a flexible and scalable platform for SNP genotyping [0.03%]
SNPlex基因分型系统：一种灵活的可拓展的单核苷酸多态性基因分型平台
Andreas R Tobler,Sabine Short,Mark R Andersen et al.
Andreas R Tobler et al.
We developed the SNPlex Genotyping System to address the need for accurate genotyping data, high sample throughput, study design flexibility, and cost efficiency. The system uses oligonucleotide ligation/polymerase chain reaction and capill...
Large-surface biosensor technology for enhanced recovery in protein characterization [0.03%]
用于蛋白质表征的大型表面生物传感器技术以实现更高的回收率
Ermias Melles,Tomas Bergman,Marie Ståhlberg et al.
Ermias Melles et al.
A large-surface biosensor technique using surface plasmon resonance (SPR) was tested for protein purification by recovery of a monoclonal antibody against human proinsulin C-peptide. Notably, both reversible attachment/desorption and actual...
Hematopoietic chimerism monitoring based on STRs: quantitative platform performance on sequential samples [0.03%]
基于STR的造血嵌合体监测：顺序样本定量平台性能评估研究
Don Kristt,Moshe Israeli,Ronit Narinski et al.
Don Kristt et al.
Hematopoietic stem cell transplantation (HSCT) creates a donor-recipient cellular chimerism in the patient, which is quantitatively assayed from peripheral blood based on STR-DNA. Since chimerism values often vary across a patient's samples...
Proteomic changes in bovine heart mitochondria with age: using a novel technique for organelle separation and enrichment [0.03%]
基于一种新型器室分离和浓缩技术的牛心脏线粒体蛋白质组变化研究
Ajay N Kiri,Hung-Cuong Tran,Kate L Drahos et al.
Ajay N Kiri et al.
Separation and enrichment of organelles from complex biological mixtures are important for proteomic analysis. Two widely used current standard techniques to isolate individual organelles include differential and density-gradient centrifuga...
Comparative Study
Journal of biomolecular techniques : JBT. 2005 Dec;16(4):371-9. DOI: 2005
Identification of ubiquitin nitration and oxidation using a liquid chromatography/mass selective detector system [0.03%]
利用液相色谱/质选检测器系统鉴定泛素硝基化和氧化形式
Donghui Yi,Patrick D Perkins
Donghui Yi
Ubiquitin is a member of the family of low-molecular-weight heat shock proteins that serve a vital role in physiological and pathological protein turnover. It appears to be one of the proteins involved in cell alterations during aging, dege...