Biological systems modeling and analysis: a biomolecular technique of the twenty-first century [0.03%]
21世纪的生物系统 Modeling与分析：一种分子技术方法
Gautam Goel,I-Chun Chou,Eberhard O Voit
Gautam Goel
It is proposed that computational systems biology should be considered a biomolecular technique of the twenty-first century, because it complements experimental biology and bioinformatics in unique ways that will eventually lead to insights...
Long-term room temperature storage of high-quality embryonic stem cell genomic DNA extracted with a simple and rapid procedure [0.03%]
一种简便快速的方法提取高质量的胚胎干细胞基因组DNA及其长期保存
Carla Tribioli,Thomas Lufkin
Carla Tribioli
A very simple procedure for the isolation of high-quality, high-molecular-weight genomic DNA from embryonic stem cells is described. The DNA is very stable once dried and can be stored for long periods of time without refrigeration. Living ...
Comparative Study
Journal of biomolecular techniques : JBT. 2006 Sep;17(4):249-51. DOI: 2006
Influence of increased CD4 cell counts on the genetic variability of hepatitis C virus in patients co-infected with human immunodeficiency virus I [0.03%]
CD4细胞增多对人类免疫缺陷病毒Ⅰ型感染患者肝炎C病毒感染性的影响
Xue-Ping Wang,Leslie Goodwin,Pamela Kahn et al.
Xue-Ping Wang et al.
In order to study the effect of increased CD4 cell counts on the biology of hepatitis C virus (HCV), we analyzed the genetic variability of HCV generated over 8 y in eight human immunodeficiency virus-1 (HIV-1) and HCV co-infected patients....
Interactions between the cytomegalovirus promoter and the estrogen response element: implications for design of estrogen-responsive reporter plasmids [0.03%]
人巨细胞病毒启动子与雌激素反应元件之间的相互作用及其对雌激素反应报告质粒设计的意义
K Derecka,C K Wang,A P F Flint
K Derecka
We aimed to produce an estrogen-responsive reporter plasmid that would permit monitoring of estrogen receptor function in the uterus in vivo. The plasmid pBL-tk-CAT(+)ERE was induced by estrogen in bovine endometrial stromal cells. When the...
Jan Kieleczawa
Jan Kieleczawa
Despite enormous progress, the DNA sequencing of difficult regions, whether they are part of complex genomes or individual constructs, still presents a significant challenge and may require many trials, brute-force, or the intervention of a...
Comparative Study
Journal of biomolecular techniques : JBT. 2006 Jul;17(3):207-17. DOI: 2006
The end of the microarray Tower of Babel: will universal standards lead the way? [0.03%]
微阵列巴别塔终结了吗？通用标准会引领出路吗？
Ernest S Kawasaki
Ernest S Kawasaki
Microarrays are the most common method of studying global gene expression, and may soon enter the realm of FDA-approved clinical/diagnostic testing of cancer and other diseases. However, the acceptance of array data has been made difficult ...
Comparison of variability associated with sample preparation in two-dimensional gel electrophoresis of cardiac tissue [0.03%]
心脏组织二维凝胶电泳中与样品制备相关的变异性的比较分析研究
Alison M Bland,Louis R D&#x;Eugenio,Melissa A Dugan et al.
Alison M Bland et al.
Variability is a major complicating factor in analysis by two-dimensional gel electrophoresis. Improvements in methodologies have focused on improving individual gel quality rather than reproducibility. We homogenized rat cardiac tissue and...
Comparative Study
Journal of biomolecular techniques : JBT. 2006 Jul;17(3):195-9. DOI: 2006
The ABRF MARG microarray survey 2005: taking the pulse of the microarray field [0.03%]
ABRF-MARG微阵列调查2005：探测微阵列领域的脉搏
Kevin L Knudtson,Herbert Auer,Andrew I Brooks et al.
Kevin L Knudtson et al.
Over the past several years, microarray technology has evolved into a critical component of any discovery-based program. Since 1999, the Association of Biomolecular Resource Facilities (ABRF) Microarray Research Group (MARG) has conducted b...
Increased protein yields from Escherichia coli using pressure-cycling technology [0.03%]
使用压力循环技术提高大肠杆菌蛋白质产量
Gary B Smejkal,Myra H Robinson,Nathan P Lawrence et al.
Gary B Smejkal et al.
Sample preparation is critical to the success of two-dimensional gel electrophoresis and other analytical methods. Pressure-cycling technology (PCT) uses alternating cycles of high and low pressure to induce cell lysis. Cell suspensions wer...
Cavan Reilly,Arvind Raghavan,Paul Bohjanen
Cavan Reilly
Cross-hybridization is the tendency for chains of nucleic acids to bind to other chains of nucleic acids that have similar but not identical sequences. This has the potential to make the interpretation of microarray experiments difficult si...