DNA sequencing research group: 2006 general survey of DNA sequencing facilities [0.03%]
DNA测序研究组：2006年DNA测序设施普查概况
David Needleman,Debbie Adam,Michelle Detwiler et al.
David Needleman et al.
Over the past few years, technological advances in automated DNA sequencing have had a profound effect on the nature of DNA sequencing laboratories. To characterize the changes occurring within DNA sequencing facilities, the DNA Sequencing ...
Comparative Study
Journal of biomolecular techniques : JBT. 2007 Apr;18(2):113-9. DOI: 2007
L T T Tran-Nguyen,K S Gibb
L T T Tran-Nguyen
Genome analysis of uncultivable plant pathogenic phytoplasmas is hindered by the difficulty in obtaining sufficient quantities of phytoplasma enriched DNA. We investigated a combination of conventional enrichment techniques such as cesium c...
Summary of the Northeast Regional Life Science Core Directors (NERLSCD) 2006 meeting [0.03%]
东北区域生命科学核心董事会议(NERLSCD) 2006年会总结报告
G S Grills,M Detwiler,T Thannhauser et al.
G S Grills et al.
Abstracts of the Association of Biomolecular Resource Facilities 2007 meeting, Tampa, Florida, USA [0.03%]
美国佛罗里达州坦帕市召开的2007年生物分子资源设施协会会议摘录摘要英文题目：Abstracts of the Association of Biomolecular Resource Facilities 2007 meeting, Tampa, Florida, USA
P. A. Binz,N. Budin,A. Niknejad et al.
P. A. Binz et al.
A number of protein and peptide identification software tools based on MS data are available to the proteomics researchers. They all share a common functionality: they process MS data and present in their output peptides and proteins that b...
Rapid and efficient MALDI-TOF MS peak detection of 2-nitrobenzenesulfenyl-labeled peptides using the combination of HPLC and an automatic spotting apparatus [0.03%]
采用HPLC与自动点样装置结合进行2-硝基苯磺酰胺标记肽的快速高效质谱检测
Tetsuo Iida,Hiroki Kuyama,Makoto Watanabe et al.
Tetsuo Iida et al.
In this paper, we report MALDI-TOF ms analysis of 2-nitrobenzenesulfenyl (NBS) labeled peptides with the powerful aid of an LC-automatic spotting system. using this approach we analyzed mammalian sera (rat and mouse) as biological samples t...
Verification of single-peptide protein identifications by the application of complementary database search algorithms [0.03%]
通过应用互补数据库搜索算法验证单肽蛋白质鉴定结果
James G Rohrbough,Linda Breci,Nirav Merchant et al.
James G Rohrbough et al.
Data produced from the MudPIT analysis of yeast (S. cerevisiae) and rice (O. sativa) were used to develop a technique to validate single-peptide protein identifications using complementary database search algorithms. This results in a consi...
ABRF ESRG 2005 study: identification of seven modified amino acids by Edman sequencing [0.03%]
ABRF 2005 ESRG研究：利用Edman测序鉴定七种修饰氨基酸
D Brune,N D Denslow,R Kobayashi et al.
D Brune et al.
Identification of modified amino acids can be a challenging part for Edman degradation sequence analysis, largely because they are not included among the commonly used phenylthiohydantion amino acid standards. Yet many can have unique reten...
J Allen Easton,Peter Thompson,Michael W Crowder
J Allen Easton
Zinc homeostasis is not well understood beyond methods of import and export. In order to better understand zinc homeostasis in Escherichia coli by identifying Zn(II)-responsive proteins, a proteomic approach was taken. Through the use of tw...
Comparative Study
Journal of biomolecular techniques : JBT. 2006 Dec;17(5):303-7. DOI: 2006
Shamim Shaikh,Louise F B Nicholson
Shamim Shaikh
We have optimized a two-plasmid Tet-On system, the regulatory plasmid and the response plasmid, to produce tightly controlled inducible expression of the gene RAGE in cell-culture models. Two sets of plasmids were constructed: set 1 (univer...
In vitro optimization of antisense oligodeoxynucleotide design: an example using the connexin gene family [0.03%]
体外反义寡核苷酸设计的优化：应用连接蛋白基因家族举例说明
Lee Yong Law,Wei V Zhang,N Susan Stott et al.
Lee Yong Law et al.
The completion of the human and mouse genomes has identified at least 20 connexin isomers in this family of intercellular channel proteins. However, there are no specific gap junction blockers or channel-blocking mimetic peptides available ...
Comparative Study
Journal of biomolecular techniques : JBT. 2006 Sep;17(4):270-82. DOI: 2006