A two-stage differential hydrogen deuterium exchange method for the rapid characterization of protein/ligand interactions [0.03%]
一种用于快速表征蛋白质/配体相互作用的两步差示氢氘交换方法
Michael J Chalmers,Scott A Busby,Bruce D Pascal et al.
Michael J Chalmers et al.
The peroxisome proliferator-activated receptor is a member of the nuclear receptor superfamily of transcriptional regulators. Regulation of the nuclear receptors occurs through changes to the structure and dynamics of the ligand-binding dom...
A novel approach for ganglioside structural analysis based on electrospray multiple-stage mass spectrometry [0.03%]
基于多重质谱的电喷雾灰质结构分析的新方法研究
Alina D Zamfir,Zeljka Vukelic,Andrea Schneider et al.
Alina D Zamfir et al.
A powerful method for detailed structural analysis based on electrospray ionization high-capacity ion-trap multiple-stage mass spectrometry (MS) is for the first time introduced in glycolipidomics. The method was optimized for accurate stru...
Yi Li,Herb E Schellhorn
Yi Li
Catalase is a commonly assayed enzyme found in many bacteria and eukaryotes. In this report, we examined the applicability of a kinetic microassay to quantify catalase from two different sources. The assay was found to be linear over a wide...
An improved protocol for coupling synthetic peptides to carrier proteins for antibody production using DMF to solubilize peptides [0.03%]
使用DMF溶解多肽以改进合成肽与载体蛋白偶联用于抗体生产的协议
Syed Salman Lateef,Shalini Gupta,Lasanthi P Jayathilaka et al.
Syed Salman Lateef et al.
We present an improved protocol for coupling synthetic peptides to carrier proteins. In this protocol, dimethyl-formamide is used as the solvent to solubilize peptides instead of phosphate-buffered saline (PBS) or 6 M guanidine-HCl/0.01 M p...
Assessment of N-glycan heterogeneity of cactus glycoproteins by one-dimensional gel electrophoresis and matrix-assisted laser desorption/ionization time-of-flight mass spectrometry [0.03%]
仙人掌糖蛋白N-糖链异质性的二维凝胶电泳和基质辅助激光解析飞行时间质谱分析
Biljana Balen,Marijana Krsnik-Rasol,Alina D Zamfir et al.
Biljana Balen et al.
Artificial environmental conditions in tissue culture, such as elevated relative humidity and rich nutrient medium, can influence and modify tissue growth and induce spontaneous changes from characteristic organization pattern to unorganize...
Big results from small samples: evaluation of amplification protocols for gene expression profiling [0.03%]
小样本大作为：基因表达谱分析的放大方案评价
Agnes Viale,Juan Li,Jay Tiesman et al.
Agnes Viale et al.
Microarrays have revolutionized many areas of biology due to our technical ability to quantify tens of thousands of transcripts within a single experiment. However, there are still many areas that cannot benefit from this technology due to ...
Efficient site-directed saturation mutagenesis using degenerate oligonucleotides [0.03%]
高效位点饱和突变筛选新技术
David L Steffens,John G K Williams
David L Steffens
We describe a reliable protocol for constructing single-site saturation mutagenesis libraries consisting of all 20 naturally occurring amino acids at a specific site within a protein. Such libraries are useful for structure-function studies...
Marcia Goldfarb
Marcia Goldfarb
This work identifies statistical algorithms which need to be included in analysis of two-dimensional gels for accurate determination of differential changes. Two-dimensional electrophoresis is a powerful tool for determining differential pr...
David Arnott,Mary Ann Gawinowicz,Jeffrey A Kowalak et al.
David Arnott et al.
Accurate protein identification sometimes requires careful discrimination between closely related protein isoforms that may differ by as little as a single amino acid substitution or post-translational modification. The ABRF Proteomics Rese...
Comparative Study
Journal of biomolecular techniques : JBT. 2007 Apr;18(2):124-34. DOI: 2007
Comparison of doxycycline delivery methods for Tet-inducible gene expression in a subcutaneous xenograft model [0.03%]
地塞米松递送方法在皮下异种移植物模型中诱导基因表达的比较研究
Christopher Cawthorne,Ric Swindell,Ian J Stratford et al.
Christopher Cawthorne et al.
Doxycycline (Dox) controlled Tet systems provide a powerful and commonly used method for functional studies on the consequences of gene overexpression/downregulation. However, whereas Dox delivery in tissue culture in vitro is relatively si...
Comparative Study
Journal of biomolecular techniques : JBT. 2007 Apr;18(2):120-3. DOI: 2007