Enrichment and detection of molecules secreted by tumor cells using magnetic reversed-phase particles and LC-MALDI-TOF-MS [0.03%]
基于磁性反相微球的肿瘤细胞分泌蛋白质组的富集及LC-MALDI-TOF-MS检测技术
Jochen F Peter,Angela M Otto,Bernhard Wolf
Jochen F Peter
Tumor cells change their genetic expression pattern as they progress to states of increasing malignancy. Investigations at the DNA and RNA level alone cannot provide all the information resulting after the translation and processing of the ...
Identification on membrane and characterization of phosphoproteins using an alkoxide-bridged dinuclear metal complex as a phosphate-binding tag molecule [0.03%]
用于磷酸化蛋白质的鉴定和表征的烷氧基桥联双核金属配合物作为磷酸结合标签分子的研究
Tsuyoshi Nakanishi,Eiji Ando,Masaru Furuta et al.
Tsuyoshi Nakanishi et al.
We have developed a method for on-membrane direct identification of phosphoproteins, which are detected by a phosphate-binding tag (Phos-tag) that has an affinity to phosphate groups with a chelated Zn2+ ion. This rapid profiling approach f...
Satya P Yadav
Satya P Yadav
Measuring the binding stoichiometry of HIV-1 Gag to very-low-density oligonucleotide surfaces using surface plasmon resonance spectroscopy [0.03%]
采用表面等离子体共振光谱法测定HIV-1Gag与极低密度的寡核苷酸基底的结合比例
Andrew G Stephen,Siddhartha A K Datta,Karen M Worthy et al.
Andrew G Stephen et al.
The interaction of the HIV Gag polyprotein with nucleic acid is a critical step in the assembly of viral particles. The Gag polyprotein is composed of the matrix (MA), capsid (CA), and nucleocapsid (NC) domains. The NC domain is required fo...
Analysis of glycoproteins in human serum by means of glycospecific magnetic bead separation and LC-MALDI-TOF/TOF analysis with automated glycopeptide detection [0.03%]
基于糖特异性磁珠分离和自动化糖肽检测的液相色谱-基质辅助激光解析电离飞行时间串联质谱分析人血清糖蛋白
Katrin Sparbier,Arndt Asperger,Anja Resemann et al.
Katrin Sparbier et al.
Comprehensive proteomic analyses require efficient and selective pre-fractionation to facilitate analysis of post-translationally modified peptides and proteins, and automated analysis workflows enabling the detection, identification, and s...
Antibody microarray analysis of inflammatory mediator release by human leukemia T-cells and human non small cell lung cancer cells [0.03%]
人类T细胞和非小细胞肺癌细胞释放炎症介质的抗体微阵列分析
Bradley H Garcia nd,Aubrey Hargrave,Aric Morgan et al.
Bradley H Garcia nd et al.
Cytokines and chemokines are responsible for regulating inflammation and the immune response. Cytokine and chemokine release is typically measured by quantitative enzyme-linked immunosorbant assay (ELISA) or Western blot analysis. To expedi...
Streamlining plant sample preparation: the use of high-throughput robotics to process echinacea samples for biomarker profiling by MALDI-TOF mass spectrometry [0.03%]
基于MALDI-TOF质谱分析的生物标志物谱图的高通量植物样品前处理技术——以紫锥菊为样本对象的研究
Leasa A Greene,Issa Isaac,Dean E Gray et al.
Leasa A Greene et al.
Several species in the genus Echinacea are beneficial herbs popularly used for many ailments. The most popular Echinacea species for cultivation, wild collection, and herbal products include E. purpurea (L.) Moench, E. pallida (Nutt.) Nutt....
A comparison of nLC-ESI-MS/MS and nLC-MALDI-MS/MS for GeLC-based protein identification and iTRAQ-based shotgun quantitative proteomics [0.03%]
基于GeLC的蛋白质鉴定和iTRAQ的反相液相芯片MALDI及ESI质谱分析的比较研究
Yong Yang,Sheng Zhang,Kevin Howe et al.
Yong Yang et al.
The use of nLC-ESI-MS/MS in shotgun proteomics experiments and GeLC-MS/MS analysis is well accepted and routinely available in most proteomics laboratories. However, the same cannot be said for nLC-MALDI MS/MS, which has yet to experience s...
A density-based proteomics sample fractionation technology: folate deficiency induced oxidative stress response in liver and brain [0.03%]
基于密度的蛋白质组学样品分离技术：叶酸缺乏诱导下的肝脏和大脑氧化应激反应
Wenkui Lan,Jayita Guhaniyogi,Marc J Horn et al.
Wenkui Lan et al.
Folate deficiency (FD) alters hepatic methionine metabolism and is associated with increased hepatocellular apoptosis. Additionally, mice deprived of folate showed increased oxidative damage in brain tissue, leading to cognitive impairment....
Loyal A Goff,Jonathan Davila,Rebecka Jörnsten et al.
Loyal A Goff et al.
Regulated mRnAs during differentiation of rat neural stem cells were analyzed using the ABi1700 microarray platform. This microarray, while technically advanced, suffers from the difficulty of integrating hybridization results into public d...