High-throughput sample preparation for protein or peptide structural characterization [0.03%]
高通量样品制备在蛋白质或肽结构表征中的应用
D G Sheer,A M Pitt
D G Sheer
High-performance liquid chromatography (HPLC) combined with mass spectrometry has become the method of choice for identifying and characterizing cell-expressed biomolecules. This technology has evolved so rapidly that efficient sample prepa...
Mass spectrometry detection and reduction of disulfide adducts between reducing agents and recombinant proteins with highly reactive cysteines [0.03%]
用于检测和减少含高度活性半胱氨酸的重组蛋白与还原剂之间硫醚加合物的质谱方法及其应用
G E Begg,D W Speicher
G E Begg
Recombinant proteins with highly reactive thiol groups can form disulfide adducts with reducing agents commonly used in protein purification, such as beta-mercaptoethanol and dithiothreitol. These adducts can interfere with protein-protein ...
Quantitative real-time polymerase chain reaction for the core facility using TaqMan and the Perkin-Elmer/Applied Biosystems Division 7700 Sequence Detector [0.03%]
利用TaqMan和佩尔金埃尔默/应用生物系统7700序列检测仪进行核心设施的定量实时聚合酶链反应技术
D S Grove
D S Grove
Quantitative real-time polymerase chain reaction (PCR) using the Perkin Elmer/Applied Biosystems Division 7700 Sequence Detector provides an accurate method for determination of levels of specific DNA and RNA sequences in tissue samples. It...
R H Angeletti
R H Angeletti
Well-designed anti-peptide antibodies provide the specificity and control for everyday and cutting-edge applications. Pragmatic peptide antigen design principles can be used to help ensure production of successful antibodies. Issues of pept...
Letter from the editor [0.03%]
编者来信
C W Naeve
C W Naeve
Intrinsic protein fluorescence interferes with detection of tear glycoproteins in SDS-polyacrylamide gels using extrinsic fluorescent dyes [0.03%]
intrinsically 為泪糖蛋白的SDS-聚丙烯酰胺凝胶电泳检测中使用extrinsically荧光染料带来的问题
Zhenjun Zhao,Yulina Aliwarga,Mark D P Willcox
Zhenjun Zhao
Intrinsic protein fluorescence may interfere with the visualization of proteins after SDS-polyacrylamide electrophoresis. In an attempt to analyze tear glycoproteins in gels, we ran tear samples and stained the proteins with a glycoprotein-...
The methodology used to measure differential gene expression affects the outcome [0.03%]
用于测量差异基因表达的方法会影响结果
Yongzeng Ding,Li Xu,Borko D Jovanovic et al.
Yongzeng Ding et al.
Confirmation of gene expression by a second methodology is critical in order to detect false-positive findings associated with microarrays. However, the impact of methodology upon the measurement of gene expression has not been rigorously e...
Comparative Study
Journal of biomolecular techniques : JBT. 2007 Dec;18(5):321-30. DOI: 2007
ABRF ESRG 2006 study: Edman sequencing as a method for polypeptide quantitation [0.03%]
ABRF 2006 ESRG研究：作为多肽定量方法的Edman测序
D C Brune,B Hampton,R Kobayashi et al.
D C Brune et al.
The Edman Sequencing Research Group (ESRG) designs studies on the use of Edman degradation for protein and peptide analysis. These studies provide a means for participating laboratories to compare their analyses against a benchmark of those...
Comparative Study
Journal of biomolecular techniques : JBT. 2007 Dec;18(5):306-20. DOI: 2007
Electrophoretic techniques applied to the detection and analysis of the human microsatellite DG10s478 [0.03%]
电泳技术在人类微卫星DG10s478的检测和分析中的应用
Clara Y Taylor,Isabel A Guillén,Marcelo Nazabal et al.
Clara Y Taylor et al.
Short nucleotide repetitions (STRs) are commonly used as genetic markers; thus their detection and analysis constitutes a very important tool for the mapping of genetic diseases, as well as for gathering information about genetic polymorphi...