Salicylhydroxamic acid functionalized affinity membranes for specific immobilization of proteins and oligonucleotides [0.03%]
用于蛋白质及寡核苷酸定向固载的水杨醛肟功能化亲和膜
Amy L Springer,Anna S Gall,Karin A Hughes et al.
Amy L Springer et al.
Immobilization of proteins and other biological macromolecules on solid supports is a method suitable for purification or screening applications in life science research. Prolinx, Inc. has developed a novel chemical affinity system that can...
Rapid analysis of protein structure and dynamics by hydrogen/deuterium exchange mass spectrometry [0.03%]
基于氢/氘交换质谱的蛋白质结构和动力学快速分析方法
Yoshitomo Hamuro,Stephen J Coales,Mark R Southern et al.
Yoshitomo Hamuro et al.
An automated approach for the rapid analysis of protein structure has been developed and used to study acid-induced conformational changes in human growth hormone. The labeling approach involves hydrogen/deuterium exchange (H/D-Ex) of prote...
The use of real-time reverse transcriptase PCR for the quantification of cytokine gene expression [0.03%]
实时反转录PCR法用于细胞因子基因表达的定量分析
L Overbergh,A Giulietti,D Valckx et al.
L Overbergh et al.
Real-time reverse transcriptase polymerase chain reaction (RT-PCR) is becoming a widely used method to quantify cytokines from cells, tissues, or tissue biopsies. The method allows for the direct detection of PCR product during the exponent...
A rapid method for manual or automated purification of fluorescently labeled nucleic acids for sequencing, genotyping, and microarrays [0.03%]
用于测序、基因型分析和微阵列的快速荧光标记核酸纯化方法
Amy L Springer,Lisa R Booth,Michael D Braid et al.
Amy L Springer et al.
Fluorescent dyes provide specific, sensitive, and multiplexed detection of nucleic acids. To maximize sensitivity, fluorescently labeled reaction products (e.g., cycle sequencing or primer extension products) must be purified away from resi...
Comparative Study
Journal of biomolecular techniques : JBT. 2003 Mar;14(1):17-32. DOI: 2003
Simple tests to detect errors in high-throughput genotype data in the molecular laboratory [0.03%]
简单的分子生物学实验室检测高通量基因型数据错误的测试方法
David J Vandenbergh,Kathrine Heron,Ryan Peterson et al.
David J Vandenbergh et al.
With the advent of high-density DNA marker data sets for the mouse and other model systems, 100 or more genotype are routinely generated from large groups of mice. Issues of the accuracy and reliability of the genotyping are extremely impor...
Angiotensin II-acetylcholine noncovalent complexes analyzed with MALDI-ion mobility-TOF MS [0.03%]
matrix辅助激光解吸离子化-离子淌度-飞行时间质谱分析血管紧张素II-乙酰胆碱非共价复合物
Amina S Woods,Katrin Fuhrer,Marc Gonin et al.
Amina S Woods et al.
Matrix-assisted laser desorption ionization-ion mobility-orthogonal time-of-flight mass spectrometry (MALDI-IM oTOF MS) is a new technique that allows laser desorbed ion to be preseparated on the basis of their shape prior to mas analysis. ...
Comparative Study
Journal of biomolecular techniques : JBT. 2003 Mar;14(1):1-8. DOI: 2003