Matthew O&#x;Donohue,Chaoming Gu,Byungsoo Kim et al.
Matthew O&#x;Donohue et al.
We demonstrate a hybrid microtubule-solid-state nanopore (MT-SSN) platform that enables label-free single-molecule analysis. Under continuous voltage bias, individual taxol-stabilized MTs are electrostatically anchored into an SSN to form a...
A Separation Buffers Platform Set to Facilitate CZE Charge Heterogeneity Method Development for Monoclonal Antibodies [0.03%]
一种用于促进单克隆抗体CZE电荷异质性方法开发的分离平台
Joanne Baxter,Kevin Waltham,Lori Fitton et al.
Joanne Baxter et al.
Monoclonal antibodies (mAbs) are complex therapeutic proteins exhibiting heterogeneity due to post-translational modifications, making charge variant analysis essential for defining critical quality attributes. Although ion-exchange chromat...
A Continuum Modeling Approach to Nanoparticle Polarizability Characterization Using Fluorescence Intensity Profiles [0.03%]
基于荧光强度谱的纳米粒子介电函数的连续介质模型研究
Mens Wout,Wood Jeffery A,Liu Chengxun et al.
Mens Wout et al.
The use of dielectrophoresis (DEP) for the manipulation of electrically polarizable particles has received a lot of interest in the past decades. Theories relying on the Clausius-Mossotti (CM) factor describe the DEP behavior of macroscale ...
A Biomimetic Protein Immobilization Method for Studying Drug-Protein Interactions Based on Affinity Capillary Electrochromatography [0.03%]
一种仿生蛋白质固定化方法及其在亲和毛细管电色谱研究药物-蛋白相互作用中的应用
Jiaping Cui,Yawen Tian,Xinru Fu et al.
Jiaping Cui et al.
In complex systems, a universal and effective method for accurately determining the dissociation constant (Kd) of drug-protein interactions (DPI) has not been identified yet. In this study, a novel biomimetic affinity capillary electrochrom...
Electrokinetic Nucleic Acid Amplification (E-NAAMP) Using Paper-PDMS Microfluidics and High-Frequency Joule Heating [0.03%]
基于纸-聚二甲基硅氧烷微流控器件的高频焦耳加热驱动核酸电泳扩增技术(E-NAAMP)
Jarad Yost,Md Nazibul Islam,Zachary Gagnon
Jarad Yost
We present a novel paper-based nucleic acid amplification (NAA) technique using electrokinetic nucleic acid amplification (E-NAAMP). In E-NAAMP, a high radio frequency (RF) potential is applied across a conductive aqueous sample to induce a...
Capillary Zone Electrophoresis for the Analysis of Phenolic Preservatives in Plasma and Insulin Formulations Using Salting-Out Assisted Liquid-Liquid Extraction [0.03%]
盐效应辅助液液萃取结合毛细管区带电泳测定血浆和胰岛素制剂中的苯酚类防腐剂
Meriem Dadouch,Yoann Ladner,Catherine Perrin
Meriem Dadouch
Phenolic preservatives are critical excipients in biopharmaceutical formulations such as insulin and its analogs. The determination of these analytes in insulin formulations and human plasma is crucial for various applications. This study r...
Improvement of Peak Integration in Capillary Electrophoresis: Reference Data Set No. 1 [0.03%]
毛细管区带电泳峰积分的改进——参考数据集1
Marlon Krompholz,Timothy Blanc,Huixin Lu et al.
Marlon Krompholz et al.
Capillary electrophoresis (CE) often provides superior separation of macromolecules such as monoclonal antibodies (mAbs), a major biopharmaceutical class, compared with liquid chromatography. However, electropherograms frequently exhibit co...
A Microfluidic Platform for Five-Order-of-Magnitude Concentration Control With Stable Droplet Size [0.03%]
一种稳定液滴尺寸的五量级浓度控制微流控平台
Sung-Jin Kim
Sung-Jin Kim
Generating monodisperse droplets with tunable concentrations is essential for a wide range of microfluidic applications. We present a platform that overcomes the trade-off between concentration range and droplet size stability using a passi...
Sensitive Analysis of Topoisomers of Large Plasmid DNA by Capillary Gel Electrophoresis With Laser-Induced Fluorescence in Uncoated Capillary [0.03%]
不用涂层的毛细管进行激光诱导荧光毛细管凝胶电泳分析大型质粒DNA的各种拓扑异构体
Yan He,John Orlet,Olivia Cunio et al.
Yan He et al.
This study presents a capillary gel electrophoresis-laser-induced fluorescence (CGE-LIF) method for analyzing topoisomers of large plasmid DNA (up to 19 kbp) using an uncoated capillary. Separation of large plasmid isoforms, particularly th...
Integrated Genetic Profiling for Cattle Parentage Testing via Next-Generation Sequencing [0.03%]
基于下一代测序的奶牛亲缘鉴定综合基因型检测方法研究
Jiaojiao Geng,Jiajun Liu,Zhentang Liu et al.
Jiaojiao Geng et al.
Cattle are frequently involved in civil disputes and offenses. Currently, the paternity testing of cattle is mainly done by analysis of dinucleotide STR (di-STR) or SNPs. However, the di-STR marker usually produces many stutter artifacts th...