Screening CZE-UV Green Method for Acetate, Propionate, Butyrate, and Valerate Analysis in Human Stool for Complementary Gut Dysbiosis Diagnosis [0.03%]
用于人体粪便中醋酸、丙酸、丁酸和戊酸分析的筛选CZE-UV绿色方法以辅助诊断肠道菌群失调
Olívia B O Moreira,Matheus Miranda Fracetti,Eliane B T Lemos et al.
Olívia B O Moreira et al.
Short-chain fatty acids present relevant function in the human body as fermentation metabolites pathways in the natural intestine microbiota activity. Their evaluations can be useful for indication as biomarkers for gut dysbiosis, provided ...
Development of Pentaplex Reverse Transcription Droplet Digital PCR Assay for Simultaneous Detection and Absolute Quantification of HIV-1, HIV-2, HCV, and HBV With Internal Control [0.03%]
用于同时检测和绝对定量HBV、HCV、HIV-1和HIV-2并含有内标的五联逆转录数字液滴PCR检测试剂盒的研制
Soo Yeon Lim,Un Na Koh,Ah Leum Kim et al.
Soo Yeon Lim et al.
This study presents the development of a pentaplex reverse transcription droplet digital polymerase chain reaction (RT-ddPCR) assay for the simultaneous detection and absolute quantification of human immunodeficiency virus (HIV)-1, HIV-2, h...
A Consecutive Separation Strategy Using a 3D-Printed Microfluidic Chip to Achieve High-Purity White Blood Cells From Blood [0.03%]
一种使用3D打印微流控芯片的连续分离策略实现高纯度的血液分离得白细胞的方法
Haoyuan Gu,Feng Yang,Chushan Gao et al.
Haoyuan Gu et al.
Label-free and direct separation of white blood cells (WBCs) remains one of the major challenges in the field of efficient leukocyte separation. To address this challenge, we propose a WBC sorting strategy based on inertial microfluidics an...
A Novel HILIC-UPLC-DAD-MS/MS Method for the Analysis of Mycosporine-Like Amino Acids and Their Quantification in Diverse Algae [0.03%]
一种新颖的亲水作用色谱-超高效液相色谱-二极管阵列检测器-串联质谱法用于分析类似麦孢素氨基酸并定量其在不同藻类中的含量
Armin Oberosler,Anastasiia Fedorova,Ignacio Zegri et al.
Armin Oberosler et al.
Mycosporine-like amino acids (MAAs) are photoprotective compounds primarily produced by marine organisms, especially red algae. Chemically, MAAs and their precursors are low-molecular-weight natural products that absorb ultraviolet radiatio...
Determination of Flunixin Meglumine in Veterinary Pharmaceutical Formulations by Capillary Electrophoresis With Capacitively Coupled Contactless Conductivity Detection [0.03%]
应用毛细管电泳-容积波导接触电阻检测法测定兽用制剂中氟尼辛葡甲胺
Letícia M de Araujo,José A Fracassi da Silva,Dosil P de Jesus
Letícia M de Araujo
This work describes a novel, simple, fast, reliable, and environmentally friendly analytical method for the determination of the anti-inflammatory and analgesic drug flunixin meglumine (FM) in veterinary pharmaceutical formulations. The met...
Probing Chiral Recognition on Amylose Tris(3,5-Dimethylphenylcarbamate) Using Cinnamyl 2-Aminoanilides: The Subtle Impact of Aliphatic Substituents [0.03%]
采用肉桂基-2-芳氨茋探针研究支链淀粉三（3，5-二甲苯基）карбамат的手性识别：脂肪烃取代基的微妙效应
Selen Gözde Kaya,Alessia Raucci,Clemens Zwergel et al.
Selen Gözde Kaya et al.
This study investigates the chiral recognition behavior of the polysaccharide‑based chiral stationary phase amylose tris(3,5‑dimethylphenylcarbamate) (Chiralpak AD‑3) under polar organic conditions, using a series of racemic cinnamyl 2‑...
A Simulation Study of Inhibition Assay With Theophylline on the Enzymatic Hydrolysis of p-Nitrophenyl Phosphate With Alkaline Phosphatase in a Continuous Mode of Electrophoretically Mediated Microanalysis [0.03%]
微电泳介导的连续流动模式微量分析中咖啡因对碱性磷酸酶水解p-硝基苯基磷酸酯反应抑制作用的计算机模拟研究
Toshio Takayanagi,Asuka Nomoto,Hitoshi Mizuguchi
Toshio Takayanagi
Inhibition assays in a continuous mode of electrophoretically mediated microanalysis (EMMA) were simulated using a MATLAB matrix. The enzymatic hydrolysis of p-nitrophenyl phosphate (NPP) with alkaline phosphatase (ALP) was adopted as a mod...
A Five-Color Multiplex Droplet Digital PCR Assay With Support Vector Regression for Forensic Age Prediction [0.03%]
用于法医年龄预测的五色多重数字PCR基因分型及其支持向量回归模型分析方法研究
Jing Zhang,Chuantong Zhao,Li Jiang et al.
Jing Zhang et al.
Current forensic methylation analysis faces challenges: Traditional SNaPshot assays often suffer from limited inhibitor tolerance and high reagent costs, whereas massively parallel sequencing (MPS) involves a labor-intensive workflow and lo...
Paper-Based Electrochemical Aptasensor Chip for Ultrasensitive Glyphosate Detection Enabled by MXene-Au and Au@NiCo LDH Nanozyme Amplification [0.03%]
基于MXene-Au和Au@NiCo层状双氢氧化物纳米酶放大作用的超灵敏草甘膦纸基电化学生物传感器芯片
Yaqi Yang,Hongxiao Sun,Ke Sun et al.
Yaqi Yang et al.
As one of the world's most widely used herbicides, glyphosate (Gly) poses considerable ecological and human health risks due to its environmental persistence and capacity to bioaccumulate through food webs in both terrestrial and aquatic ec...
Integrated Purification and Desalting of Plasmid DNA Using Monolith Columns [0.03%]
采用整体柱同时纯化和脱盐质粒DNA
Rok Miklavčič,Tadej Sever,Polona Skrt et al.
Rok Miklavčič et al.
Efficient purification and desalting of nucleic acids are essential unit operations in downstream processing (DSP). Conventional methods offer good solutions for either desalting (e.g., tangential flow filtration, dialysis and size exclusio...