The position of the AUG start codon in MFG-based γ-retroviral vectors has a dramatic effect on translation-dependent protein expression [0.03%]
MFG基γ-逆转录病毒载体中的AUG起始密码子位置对依赖翻译的蛋白质表达具有重大影响
Timothy L Frankel,Ling Zhang,William R Burns et al.
Timothy L Frankel et al.
Background: In the past three decades, much advancement has been made in γ-retroviral vector mediated gene transfer. One widely used vector design is based on the MFG vector, which uses the Moloney murine leukemia virus ...
Immunity against a therapeutic xenoprotein/Fc construct delivered by gene transfer is reduced through binding to the inhibitory receptor FcγRIIb [0.03%]
通过基因转移递送的治疗性异源蛋白/Fc融合体可以与抑制受体FcγRIIb结合，从而降低免疫反应
Yaming Liang,Hongmin Qiu,Yelena Glinka et al.
Yaming Liang et al.
Background: Therapeutic xenoproteins are immunogenic and can induce neutralizing antibodies. When delivered by intramuscular injection of a plasmid vector, this mimics classical DNA vaccination. To demonstrate this, we ch...
Sleeping Beauty-mediated correction of Fanconi anemia type C [0.03%]
Sleeping Beauty 介导的C型范可尼贫血症的基因校正
Kendra A Hyland,Erik R Olson,Karl J Clark et al.
Kendra A Hyland et al.
Background: The Sleeping Beauty (SB) transposon system can insert defined sequences into chromosomes to direct the extended expression of therapeutic genes. Our goal is to develop the SB system for nonviral complementatio...
Intracellular trafficking and fate of chimeric adenovirus 5/F35 in human B lymphocytes [0.03%]
人B淋巴细胞中嵌合型腺病毒5型/35型的胞内运输及命运决定机制研究
Mélanie Samson,Daniel Jung
Mélanie Samson
Background: Investigation of the molecular processes that control the development and function of lymphocytes is essential for our understanding of humoral immunity, as well as lymphocyte-associated pathogenesis. Adenovir...
Mechanisms of polyethylenimine-mediated DNA delivery: free carrier helps to overcome the barrier of cell-surface glycosaminoglycans [0.03%]
聚乙烯亚胺介导的DNA递送机制：游离载体有助于克服细胞表面糖胺聚糖屏障
Martina Hanzlíková,Marika Ruponen,Emilia Galli et al.
Martina Hanzlíková et al.
Background: Polyethylenimine (PEI) polyplexes mediate efficient gene transfer only at high +/- charge ratios at which free noncomplexed PEI is present. The excess of PEI gives polyplexes a positive surface charge that pla...
Therapeutic ultrasound promotes plasmid DNA uptake by clathrin-mediated endocytosis [0.03%]
治疗性超声通过网格蛋白介导的内吞作用促进质粒DNA吸收
Daisy Maria Bentes Paula,Valderez Bastos Valero-Lapchik,Edgar Julian Paredes-Gamero et al.
Daisy Maria Bentes Paula et al.
Background: Ultrasound (US) has been widely used to improve the efficiency of nonviral vector transfection. The mechanism of plasmid uptake is usually attributed to sonoporation, although there is not clear evidence for t...
Sustained, high transgene expression in liver with plasmid vectors using optimized promoter-enhancer combinations [0.03%]
利用优化的启动子-增强子组合使肝细胞中质粒载体介导的基因高效持久表达
Terese Magnusson,Rudolf Haase,Martin Schleef et al.
Terese Magnusson et al.
Background: Plasmid-based gene therapy approaches often lack long-term transgene expression in vivo as a result of silencing or loss of the vector. One way to overcome these limitations is to combine nonsilenced promoters...
Gene therapy with adenovirus-delivered indoleamine 2,3-dioxygenase improves renal function and morphology following allogeneic kidney transplantation in rat [0.03%]
腺病毒递送吲哚胺2，3-双氧酶基因治疗改善大鼠异体肾移植的肾功能和形态学损伤
Diana Vavrincova-Yaghi,Leo E Deelman,Harry Goor et al.
Diana Vavrincova-Yaghi et al.
Background: Indoleamine 2,3-dioxygenase (IDO), the rate-limiting enzyme in the tryptophan catabolism, has recently emerged as an important immunosuppressive enzyme involved in the regulation of both physiologic (maternal ...
Hepatic control elements promote long-term expression of human coagulation factor IX gene in hydrodynamically transfected mice [0.03%]
肝控制元件促进水动力转染的小鼠血栓形成素IX基因的长期表达
Hong Sung Kim,Jong Chul Kim,Yeon Kyung Lee et al.
Hong Sung Kim et al.
Background: Long-term expression of the delivered target gene is critical for successful gene therapy. Recently, hepatic control region I (HCR I) originating from the apolipoprotein (apo)C-I pseudogene was shown to be a c...
Intravenous injection of irradiated tumor cell vaccine carrying oncolytic adenovirus suppressed the growth of multiple lung tumors in a mouse squamous cell carcinoma model [0.03%]
携带肿瘤溶瘤腺病毒的辐照肿瘤细胞疫苗静脉注射抑制小鼠肺鳞癌模型中多发肿瘤生长
Aya Saito,Naoya Morishita,Chihomi Mitsuoka et al.
Aya Saito et al.
Background: Although cancer therapy using replication-selective oncolytic adenoviruses has been available for many years, its anti-tumor efficacy is suboptimal as a result of low and nonspecific infectivity that depends o...