Cell Cycle Synchronization of the Murine EO771 Cell Line Using Double Thymidine Block Treatment [0.03%]
双胸腺嘧啶阻滞法同步EO771小鼠细胞系的细胞周期
Marie Goepp,Delphine Le Guennec,Adrien Rossary et al.
Marie Goepp et al.
This study shows that double thymidine block treatment efficiently arrests the EO771 cells in the S-phase without altering cell growth or survival. A long-term analysis of cell behavior, using 5(6)-carboxyfluorescein diacetate N-succinimidy...
Cas9 Cuts and Consequences; Detecting, Predicting, and Mitigating CRISPR/Cas9 On- and Off-Target Damage: Techniques for Detecting, Predicting, and Mitigating the On- and off-target Effects of Cas9 Editing [0.03%]
Cas9切割及其后果：检测、预测和缓解CRISPR/Cas9的靶向和非特异性脱靶损伤：用于检测、预测和减轻Cas9编辑的靶向和非特异性脱靶效应的技术
Anthony Newman,Lora Starrs,Gaetan Burgio
Anthony Newman
Large deletions and genomic re-arrangements are increasingly recognized as common products of double-strand break repair at Clustered Regularly Interspaced, Short Palindromic Repeats - CRISPR associated protein 9 (CRISPR/Cas9) on-target sit...
Nutrient Sensing by Histone Marks: Reading the Metabolic Histone Code Using Tracing, Omics, and Modeling [0.03%]
组蛋白修饰感应营养物质：利用示踪、组学和建模读取代谢组蛋白密码
Scott E Campit,Alia Meliki,Neil A Youngson et al.
Scott E Campit et al.
Several metabolites serve as substrates for histone modifications and communicate changes in the metabolic environment to the epigenome. Technologies such as metabolomics and proteomics have allowed us to reconstruct the interactions betwee...
Lifespan Extension Via Dietary Restriction: Time to Reconsider the Evolutionary Mechanisms? [0.03%]
通过饮食限制延长寿命：是重新考虑进化机制的时候了吗？
Joshua P Moatt,Eevi Savola,Jennifer C Regan et al.
Joshua P Moatt et al.
Dietary restriction (DR) is the most consistent environmental manipulation to extend lifespan. Originally thought to be caused by a reduction in caloric intake, recent evidence suggests that macronutrient intake underpins the effect of DR. ...
Competitive and Clonal Dominance Behavior: Raising Awareness of Their Role in Shape Generation [0.03%]
竞争性和克隆主导行为：提高它们在形态发生中的作用意识
Sabine Hoffmeister-Ullerich
Sabine Hoffmeister-Ullerich
Gene regulatory networks, which are crucial for the proper development of organisms, are extensively studied. From these investigations, coordinative mechanisms are shown to be instructive for further development. Competitive mechanisms as ...
Neuronal Compartmentalization: A Means to Integrate Sensory Input at the Earliest Stage of Information Processing? [0.03%]
神经元隔室化：整合感觉输入的最早信息处理机制？
Renny Ng,Shiuan-Tze Wu,Chih-Ying Su
Renny Ng
In numerous peripheral sense organs, external stimuli are detected by primary sensory neurons compartmentalized within specialized structures composed of cuticular or epithelial tissue. Beyond reflecting developmental constraints, such comp...
Eggshell Biliverdin as an Antioxidant Maternal Effect: Biliverdin as an Antioxidant Resource in Oviparous Animals [0.03%]
蛋壳胆绿素作为抗氧化的母体效应：胆绿素作为卵生动物的抗氧化资源
Judith Morales
Judith Morales
In this essay, the hypothesis that biliverdin pigment plays an antioxidant role in the avian eggshell is proposed. Due to its ability to scavenge free radical species and to reduce mutation, biliverdin potentially counteracts the oxidative ...
Competitive and Coordinative Interactions between Body Parts Produce Adaptive Developmental Outcomes [0.03%]
竞争和协调的机体互动产生适应性的发展结果
Richard Gawne,Kenneth Z McKenna,Michael Levin
Richard Gawne
Large-scale patterns of correlated growth in development are partially driven by competition for metabolic and informational resources. It is argued that competition between organs for limited resources is an important mesoscale morphogenet...
Rajivgandhi Sundaram,Dileep Vasudevan
Rajivgandhi Sundaram
Chromatin structure and dynamics regulate key cellular processes such as DNA replication, transcription, repair, remodeling, and gene expression, wherein different protein factors interact with the nucleosomes. In these events, DNA and RNA ...