Chronofluorometry Based on a Facile Fluorescent Probe for Rapid Detection of Fractional Exhaled Nitric Oxide and Bioimaging in Live Cells [0.03%]
一种简便型荧光探针的克罗诺佛洛里计用于快速检测呼出的一氧化氮并进行活细胞成像
Hai Wu,Yuanyuan Li,Hao Qin et al.
Hai Wu et al.
Fractional exhaled nitric oxide (FeNO) is an established biomarker of type 2 airway inflammation in asthma. The advancement of rapid and sensitive FeNO detection technology is crucial for improving the diagnosis and treatment of patients wi...
Ali Gholizadeh,Gabriel Mazzucchelli,Ana Amoroso et al.
Ali Gholizadeh et al.
Sample preparation is often a critical and labor-intensive step in molecular biology and analytical chemistry. It bottlenecks biological assays, where liquid-handling speed and technique influence the outcome. While automation improves effi...
Underwater Gas Chromatograph-Quadrupole Mass Spectrometer for In Situ Analysis of VOCs in Deep Sea [0.03%]
深海原位挥发性有机物分析的水下气相色谱-四级杆质谱仪
Shihao Lu,Kun Ding,Qixin Liu et al.
Shihao Lu et al.
An underwater gas chromatograph-quadrupole mass spectrometer (UGC-qMS) for in situ qualitative and quantitative analysis of volatile organic compounds (VOCs) in the deep sea was developed and evaluated. The UGC-qMS utilizes a membrane inlet...
Precise Determination of CpG Methylation through Entropy-Driven Cascade Circuit [0.03%]
基于熵驱动级联电路的CpG岛甲基化精准检测方法
Xiaohui Chen,Haixia Liu,Ningyu Ma et al.
Xiaohui Chen et al.
Aberrant methylation of a particular region plays a crucial role in the occurrence and development of malignant tumors. However, the sensitive and precise determination of DNA methylation from clinical samples remains challenging. Here, we ...
Research on the Extraction of Characteristic Information and Similarity Evaluation Method for Relative Intensity Information between Characteristic Peaks in Raman Spectra [0.03%]
基于拉曼光谱特征峰相对强度信息的相似性评价方法研究
Wendong Xue,Xiaoxing Yan,Benneng Chen et al.
Wendong Xue et al.
Automatic identification technology of Raman spectra is crucial for the accuracy and stability of qualitative Raman detection analysis and remains a technical challenge. The Relative Intensity Information between Characteristic Peaks (RIICP...
Bottom-Up Structural Analysis for Natural Products by Identifying Fragment Ions Resulted from Gas Phase Collision-Induced C-C Bond Fission [0.03%]
基于气相碰撞诱导C-C键断裂碎片离子的天然产物底部向上结构分析
Qian Wang,Ke Zhang,Jingjing Shi et al.
Qian Wang et al.
Structural degradation is a fundamental principle for identifying complicated structures. Except for the comparable ability with chemical degradation reactions to facilitate C-X (X = N, O, and S) bond fission, gas phase ion dissociation als...
Microwave-Assisted Rapid Synthesis of Near-Infrared Light-Responsive Pd Single-Atom Nanozymes for Colorimetric Detection of Acetylcholinesterase Activity [0.03%]
微波辅助近红外光响应钯单原子纳米酶的快速制备及其比色检测乙酰胆碱酯酶活性应用
Jing Ye,Meihua Hu,Qingqing Chen et al.
Jing Ye et al.
Single-atom nanozymes (SAzymes) have been widely utilized as promising sensing platforms for numerous analytical applications. However, the preparation of SAzymes usually needs tedious heating treatment, and their enzyme-mimicking activitie...
Pheno-Molecular Testing Enabled by Multiplex Droplet Digital PCR for Rapid and High-Content Detection of Carbapenem-Resistant Enterobacterales [0.03%]
基于多重液滴数字pcr的表型分子联检技术实现碳青霉烯耐药肠杆菌科细菌的快速高内涵检测
Ziyi Zhang,Dongyang Cai,Ting Zhang et al.
Ziyi Zhang et al.
Pheno-molecular testing evaluates bacterial responses to antibiotics via quantitative nucleic acid detection, thus enabling rapid antibiotic susceptibility testing by eliminating the time-consuming isolation culture step. Carbapenems exert ...
Cascade Signal Amplification of Time-Resolved Fluorescence Immunoassay through Enzyme Catalysis Coupled with Dissolution Enhancement [0.03%]
基于酶催化解离增强的时间分辨荧光级联信号放大免疫测定法
Zhenlong Lu,Jianan Niu,Yingxia Li et al.
Zhenlong Lu et al.
Time-resolved fluorescence immunoassay (TRFIA) has found wide application in various fields due to its low background noise characteristics. However, the limited attachment of lanthanide ion complexes onto antibodies restricts the signal in...
Advancing Cryo-EM and Cryo-ET through Innovation in Sample Carriers: A Perspective [0.03%]
通过样品承载器的创新推动 cryo-em 和 cryo-et 的发展：透视观点论文
Navya Premaraj,Ron M A Heeren,Raimond B G Ravelli et al.
Navya Premaraj et al.
Cryo-electron microscopy (cryo-EM) and cryo-electron tomography (cryo-ET) have revolutionized structural biology by enabling high-resolution imaging of biomolecules and cellular structures. However, traditional sample carriers, such as copp...