Adsorption-Based Aggregation-Induced Emission on Nanoparticles: A Promising Strategy for Generic Homogeneous Fluorescence Assay Platforms [0.03%]
基于吸附的聚集诱导纳米颗粒发光：通用型均相荧光分析平台的一项有前景的技术
Danni Zhao,Turghun Muhammad,Haidong Li et al.
Danni Zhao et al.
Aggregation-induced emission (AIE), a "zero background" luminescent phenomenon, shows great potential for fluorescent analytical methods. Traditionally, AIE luminogen aggregation is achieved by modifying solvent polarity. However, analyte c...
Cross-Device Risk Calibration for Surface-Enhanced Raman Spectroscopy-Based Urologic Disease Classification [0.03%]
基于表面增强拉曼光谱的泌尿系统疾病分类的跨设备风险校准
Huafeng She,Fuqiang Wang,Xin Bai et al.
Huafeng She et al.
Surface-enhanced Raman spectroscopy (SERS) combined with deep learning offers a noninvasive route for molecular screening of urologic diseases. Its broader analytical utility, however, is constrained by two coupled challenges: overconfident...
Classification of N-Nitrosamines and Other Nitroso Compounds by Using Tandem Mass Spectrometry Experiments Based on Collision- Activated Dissociation [0.03%]
基于碰撞激活解离的串联质谱实验的N-亚硝胺及其他亚硝基化合物分类法
Annika M Little,Meghan A Cortez,David S Toba-Veloza et al.
Annika M Little et al.
A method is reported for the general detection of nitroso compounds by using MSn (n = 2 or 3) experiments based on collision-activated dissociation (CAD). The fragmentation reactions of 36 protonated nitroso model compounds, supplemented by...
Ultrasensitive SERS Detection and AI-Assisted Differentiation of Structurally Similar Pollutants on Flexible Aluminum Substrates [0.03%]
基于柔性铝基底的类似污染物的超灵敏SERS检测及AI辅助鉴别技术
Jayasree Kumar,Santhoshkumar S,Cathrin Lims Selvakumar et al.
Jayasree Kumar et al.
Surface-enhanced Raman spectroscopy (SERS) achieves ultrasensitive molecular detection; however, reliable discrimination of structurally similar analytes remains a critical challenge due to spectral overlap. Here, we report a flexible alumi...
Quantitative Single-Cell Multielemental Analysis Using Metal-Loaded Microparticles: toward a Universal Quantification Workflow in Mass Cytometry [0.03%]
使用金属载荷微粒进行定量单细胞多元素分析：朝向质谱流式细胞术的通用量化工作流程发展
Ángela de la Rosa-Díaz,Carlos López-Portugués,Mario Corte-Rodríguez et al.
Ángela de la Rosa-Díaz et al.
The use of lanthanide-tagged antibodies enables multiplexed biomarker monitoring in individual cells by mass cytometry. Here, we show the design and application of a quantitative workflow that enables multiple-element determination in singl...
Linlin Wang,Gabriel Castro,Obtin Alkhamis et al.
Linlin Wang et al.
Fentanyl and its analogs pose considerable dangers to public health and safety, and the development of simple tests that can detect these harmful substances accurately and rapidly could control their currently unchecked spread and thereby s...
HaloTag-Based FACS-Compatible Assay for Intracellular Haloalkane Detection with a Potential Application to Halogenase Screening [0.03%]
基于HaloTag的用于细胞内 halo烷检测并可用于卤代酶筛选的FACS相容性实验
Hannes Meinert,Hannes Wolfgramm,Hannah Meier et al.
Hannes Meinert et al.
Halogenases are attractive catalysts to facilitate asymmetric Csp3-halogenation of organic molecules. Traditional halogen insertion reactions employ hazardous reagents and are notoriously unselective, but asymmetric halogenation of drug mol...
Single-Cell-Derived Rabbit mAb-Based Immunoassay for Simultaneous Detection of Florfenicol and Florfenicol Amine [0.03%]
基于兔单克隆抗体的氟喹诺酮和氟喹诺酮胺同时免疫检测方法
Peipei Li,Yantong Pan,Yuan Li et al.
Peipei Li et al.
Previously, we speculated that rabbits could produce more diverse and higher-affinity antibodies for small molecules due to their specific, unique antibody structure and antibody evolution mechanism. Here, using a single antibody-secreting ...
Nonconjugated Alkyl Chains in Ionic Molecular Rotor Fluorophores: An Effective Modulating Moiety for Amyloid-β Probes [0.03%]
非共轭烷基链作为离子分子陀螺荧光探针的有效调节基团：用于检测淀粉样蛋白-β的探针
Zhenyu Zhang,Junxin Yi,Zhang Lin et al.
Zhenyu Zhang et al.
Developing molecular structural tuning strategies for ionic molecular rotor (MR)-based β-amyloid (Aβ) fluorescent probes is essential for balancing high sensitivity, signal-to-background ratio (SBR) in wash-free mode, and blood-brain barr...
Formic Acid Enhanced Single-Pot Sample Preparation to Improve Identification Coverage of Membrane Proteome of Laser Capture Microdissected Tissue Sections [0.03%]
用于改进激光捕获显微切割组织切片膜蛋白组鉴定覆盖率的甲酸辅助单锅样品前处理方法
Yingyun He,Xinyu He,Dan Liu et al.
Yingyun He et al.
To interpret the heterogeneous multicellular microenvironment in biological tissues, cell type-resolved global proteome profiling has attracted great attention. Due to its high compatibility with mass spectrometry (MS), n-Dodecyl-d-maltosid...