Current Standards and Perspectives in Proteomics for Cardiac Amyloidosis [0.03%]
心脏淀粉样变性蛋白组学的当前标准和展望
Francesco Greco,Ellen McPhail,Diana Canetti et al.
Francesco Greco et al.
Amyloidosis is a heterogeneous group of protein misfolding disorders characterized by the deposition of amyloid fibrils in tissues, which causes progressive organ dysfunction. Among the various forms, cardiac involvement by transthyretin (A...
Toward Reliable Environmental Monitoring with Potentiometric Ion Sensors: Unveiling the Effects of Humic Acids and Developing Antifouling Sensors Based on a Poly(vinylidene fluoride) Coating [0.03%]
基于聚偏二氟乙烯涂层的抗 fouling 传感器的研制及腐殖酸的影响研究：致力于可靠地使用离子选择性电极进行环境监测
Yuanxin Liu,Zhibo Liao,Wei Yang et al.
Yuanxin Liu et al.
Although potentiometric ion sensors have attracted growing interest in the environmental analysis of water quality, reliable long-term continuous monitoring with these sensors remains a great challenge. Fouling of the sensor membranes can s...
One Probe, Two Readouts: A Fluorescent Nanozyme Facilitates Cascade Catalysis and On-Demand Bimodal Detection of Hepatic ALT and AST [0.03%]
一探双读：荧光纳米酶级联催化及ALT和AST的按需双模式检测
Bo Wang,Linjie Wang,Xinyu Chen et al.
Bo Wang et al.
Probing the activities of alanine aminotransferase (ALT) and aspartate aminotransferase (AST) is of significance for clinical diagnosis of hepatic diseases and monitoring of therapeutic effects. Traditional detection methods often rely on a...
Covalent Organic Framework with Acid Precipitation Enhances Microprotein Enrichment and Discovery of Ferroptosis-Associated Microproteins [0.03%]
酸沉淀增强共价有机框架提高微肽富集及铁死亡相关微肽发现能力
Fengming Li,Jingchen Sui,Xiaoqian Wang et al.
Fengming Li et al.
Microproteins, encoded by small open reading frames (sORFs), are polypeptides with fewer than 100 amino acids with unique structural and functional characteristics. Protein mass spectrometry is currently the de facto approach to verify the ...
Advancing Collision-Induced Affinity Selection Mass Spectrometry for Quantitative Ligand Analysis in Complex Mixtures [0.03%]
用于复杂混合物中配体定量分析的碰撞诱导亲和质谱技术的发展
Xinru Xue,Ronald J Quinn,Bernd H A Rehm et al.
Xinru Xue et al.
Native mass spectrometry (MS) is a powerful technique for studying protein-ligand interactions in their native states by employing soft electrospray ionization (ESI). It enables the direct observation of all species in equilibrium within a ...
Ultrarapid Collection of High-Concentration Viral Samples from Small-Volume Exhaled Breath for Respiratory Virus Surveillance [0.03%]
一种从呼出气体中收集高浓度病毒样本进行呼吸道病毒监测的超快速方法
Weihao Li,Han Gao,Mengqing Cheng et al.
Weihao Li et al.
Rapid and sensitive detection of airborne respiratory viruses from exhaled breath is essential for early diagnosis and outbreak control, yet current strategies suffer from low capture efficiency and sample dilution. Here, we present a fully...
Measuring the Degree of Labeling of Antibody-Dye Conjugates with a Single-Molecule-Sensitive Digital Flow Cytometer [0.03%]
利用单分子灵敏数字流式细胞仪测量抗体-染料偶联物的标记度
Yuanhua Cheng,Mengxia Zhao,Sebastien Barcy et al.
Yuanhua Cheng et al.
Antibody-dye conjugates are an essential tool in biomedical research and are widely used in flow cytometry, immunofluorescence imaging, and ELISAs. The degree of labeling (DOL), often referred to as the F/P (fluorophore-to-protein) ratio, w...
Streamlined Digital Microfluidics-Mass Spectrometry Strategy for Extracellular Vesicle Enrichment and Lipid Profiling [0.03%]
一种用于外泌体富集和脂质谱型分析的数字微流控-质谱策略
Menglei Zhao,Hang Li,Yudan Ma et al.
Menglei Zhao et al.
Extracellular vesicles (EVs) are nanoscale mediators of intercellular communication that contribute to disease processes, such as tumor progression and immune regulation. However, EV lipidomics remains constrained by conventional isolation ...
Multicomponent Gas Standards for Hydrogen Purity Analysis According to ISO 14687 Grade D [0.03%]
ISO 14687第D级氢纯度分析多组分气体标准品
Verena Reiter,Lea A Brandner,Sebastian Scheikl et al.
Verena Reiter et al.
ISO 14687 sets strict impurity limits for hydrogen used across a wide range of applications, including fuel cell road vehicles (Grade D). Ensuring compliance with these thresholds requires precise analytical methods and certified gas standa...
Real-Time Acoustic Spectroscopy of Nanoparticle Sedimentation in Microfluidic Systems Using Phononic Crystal-Based Ultrasonic Interferometry [0.03%]
基于声子晶体超声干涉技术的微流控系统中纳米颗粒沉降实时光谱检测技术研究
Gabriel Alejandro Medel Méndez,David Martínez Esquivel,Alfredo R Vilchis-Néstor et al.
Gabriel Alejandro Medel Méndez et al.
Real-time quantification of nanoparticle sedimentation is critical for predicting colloidal stability, yet most optical or centrifugation-based techniques require dilution, labeling, or long acquisition times. We report a defect-engineered ...