Inversion on the Extinction Coefficient of Oil Film in the Visible Wavelengths Based on Fluorescence Spectroscopy with Differential Thickness and Observation Angles [0.03%]
基于差值厚度和观测角度的荧光光谱法测量油膜消光系数的倒置现象研究可见光波段
Ming Xie,Ying Li,Yong Wang et al.
Ming Xie et al.
This study developed a novel method for the inversion on the extinction coefficient of oil film in visible wavelengths based on fluorescence spectroscopy. A new analytical model that takes the observation angles into account is established ...
Effects of Chemical Cross-Linking on the Structure of Proteins and Protein Assemblies [0.03%]
化学交联对蛋白质和蛋白质聚合体结构的影响
Tamar Tayri-Wilk,Nir Kalisman,Uri Raviv
Tamar Tayri-Wilk
Chemical cross-linking is frequently used in structural biology for protein stabilization and probing protein structures, often in combination with mass spectrometry (XL-MS). These applications assume that chemical cross-linking does not si...
Development of a Protein-Free Nucleic Acid Lateral Flow Assay for Trichomonas vaginalis [0.03%]
一种用于检测阴道毛滴虫的无蛋白核酸 lateral flow 试纸条的发展
Christine Aubrey C Justo,Miriam Jauset-Rubio,Vasso Skouridou et al.
Christine Aubrey C Justo et al.
In line with the global research priorities on sexually transmitted infections (STIs), we developed a molecular point-of-care test (POCT) for the parasite Trichomonas vaginalis that causes the STI trichomoniasis. Trichomoniasis remains the ...
Robust Chemical Cross-linking of Proteins and Protein Complexes via SuFEx Click Chemistry [0.03%]
利用SuFEx点击化学对蛋白质及蛋白复合物进行稳定的交联反应
Xiaoya Li,Kai Li,Xuechun Yuan et al.
Xiaoya Li et al.
Chemical cross-linking of proteins coupled with mass spectrometry (CXMS) has emerged as a highly efficient tool for elucidating the structures of proteins and protein complexes. This technology offers valuable insights into protein conforma...
An Approach for Measuring Extracellular Vesicle Size Using the Attenuation-Velocity Change Ratio of SH-SAW Biosensors [0.03%]
基于衰减-频率变化比的SH-SAW生物传感器微泡尺寸检测方法研究
Chia-Hsuan Cheng,Hiromi Yatsuda,Szu-Heng Liu et al.
Chia-Hsuan Cheng et al.
Large EVs (lEVs) and small EVs (sEVs) are distinct extracellular vesicles (EVs) found in physiological fluids with different size ranges, biogenesis pathways, and biomarker cargoes. This study introduces a novel approach using a shear horiz...
Collisional Cross-Section Prediction for Multiconformational Peptide Ions with IM2Deep [0.03%]
基于IM2Deep的多构象肽离子碰撞截面预测
Robbe Devreese,Alireza Nameni,Arthur Declercq et al.
Robbe Devreese et al.
Peptide collisional cross-section (CCS) prediction is complicated by the tendency of peptide ions to exhibit multiple conformations in the gas phase. This adds further complexity to downstream analysis of proteomics data, for example for id...
O-Glycosylated RNA Identification and Prediction by Solid-Phase Chemoenzymatic TnORNA Method and PONglyRNA Tool [0.03%]
基于固相化学酶法TnORNA和PONglyRNA工具的O-糖基化RNA识别与预测
Jiajia Li,Linshu Wang,Jianbo Deng et al.
Jiajia Li et al.
Recent studies have shown that the cell surface undergoes post-transcriptional modification by N-linked glycosylation. However, the question of whether RNA can be glycosylated by O-glycans remains to be explored. The presence of O-glycosyla...
A Retention-Monitoring Assay Based on Logic Profiling Nucleic Acid Framework for Accurate Identification of Extracellular Vesicles [0.03%]
基于逻辑分析的核酸框架构建的监测保藏性的测定方法用于精确鉴定细胞外囊泡
Xu Yao,Yuxin Wang,Xinming Luo et al.
Xu Yao et al.
Extracellular vesicles (EVs) hold promise as a new generation of noninvasive cancer diagnostic biomarkers, but the complexity and heterogeneity of clinical samples limit the detection accuracy and clinical applicability. Herein, we propose ...
Deep Learning-Based Classification of NSCLC-Derived Extracellular Vesicles Using AFM Nanomechanical Signatures [0.03%]
基于深度学习的AFM纳米力学特征在非小细胞肺癌来源的外泌体分类中的应用
Soohyun Park,Youngkyu Kim,Jung-Hee Kim et al.
Soohyun Park et al.
Nonsmall cell lung cancer (NSCLC) remains a leading cause of cancer-related mortality, with liquid biopsy emerging as a promising tool for noninvasive diagnostics. Extracellular vesicles (EVs) serve as molecular messengers of the tumor micr...
Enhancing Classification Robustness in Real-Time Digital PCR via Amplification Curve Regulation and Clustering Analysis [0.03%]
基于扩增曲线调节和聚类分析的数字PCR实时分类鲁棒性增强方法研究
Dongshu Li,Chuanyu Li,Qi Yang et al.
Dongshu Li et al.
Real-time digital PCR technology holds significant promise in nucleic acid detection. However, amplification heterogeneity will hinder the effective separation of positive and negative amplification curves, leading to partition misclassific...