A Label-Free Method Combining Chromatography Enrichment with N-Terminal Specific Proteolysis Enables Sensitive Identification of Tn/sTn Glycosites [0.03%]
基于色谱富集和N端特异性酶切的非标记糖基化位点鉴定方法
Zhongyu Wang,Yan Wang,Taiheng Ma et al.
Zhongyu Wang et al.
Tn (GalNAcα1-O-S/T) and sTn (Neu5Acα2-6GalNAcα1-O-S/T) antigens, the immature O-glycans, are strongly correlated with cancer aggressiveness. Tn and sTn antigens exhibit significant differences between patients and healthy people, and the...
Colorimetric-Assisted Photoelectrochemical Sensing for Dual-Mode Detection of Neuron-Specific Enolase via the Photoanode-Photocathode System [0.03%]
基于光阳极/光电阴极系统的肌醇神经特异性烯醇化酶双模式检测的比色辅助型光电化学传感法
Hanyu Wang,Tong Zhang,Xin Chen et al.
Hanyu Wang et al.
Convenient and accurate detection of neuron-specific enolase (NSE) is crucial for small cell lung cancer. However, due to the shortcomings of a single detection mode such as insufficient reliability, a dual-mode signal response system based...
Direct, Selective, and Rapid Measurement of HCN at Sub-ppb Levels Using HiT-CD-IMS: Applications to Cell Culture and Breath Analysis [0.03%]
基于HiT-CD-IMS的HCN皮摩尔级直接、快速、选择性测量及其在细胞培养和呼吸分析中的应用
Mahmoud Tabrizchi,Brian De La Franier,Sandro Spagnolo et al.
Mahmoud Tabrizchi et al.
This study demonstrates the use of a research-grade ion mobility spectrometer combined with a high-temperature negative corona discharge ionization source (HiT-CD-IMS) to detect hydrogen cyanide (HCN) emitted by Pseudomonas aeruginosa (PA) ...
Visualization of Immune Rejection: Noninvasive Imaging/Efficacy Evaluation of Immune Rejection after Liver Transplantation via Stable NIR-II Scaffolds [0.03%]
基于稳定近红外二区支架的肝移植后免疫排斥反应的无创成像/疗效评估研究
Li Xu,Hao-Ming Chen,Zuo-Jia Qin et al.
Li Xu et al.
Liver transplantation is the only definitive treatment to cure liver failure in the clinic. However, patients undergoing allogeneic liver transplantation often experience immune rejection, which can lead to significant liver damage or even ...
Fast Taylor Dispersion Analysis for Minimizing Protein Adsorption Effects [0.03%]
快速泰勒分散分析以最小化蛋白质吸附效应
Ruben Szabó,Melinda Andrási,Attila Gáspár
Ruben Szabó
Taylor Dispersion Analysis (TDA) is performed using capillaries with a small inner diameter. However, the strong tendency of proteins to adhere to the capillary walls can distort peaks, reduce reproducibility, and compromise accuracy. In th...
Peroxynitrite-Activated Conditional Chemical Probe for the Affinity-Based Protein Modification [0.03%]
基于亲和性的蛋白质修饰的过氧亚硝酸盐激活条件化学探针
Chien-Chi Wu,Szu-Hsien Lee,Ting-Ju Huang et al.
Chien-Chi Wu et al.
Affinity-based protein labeling chemical probes are invaluable tools for the selective chemical modification of native proteins. This protein labeling strategy generally relies on "always ON" reactive electrophiles to achieve protein modifi...
Polydopamine-Mediated Flexible Spatial Barrier Facilitates Multilayer Assembly of Nanozymes to Co-Strength Point-of-Care Immunoassays [0.03%]
聚多巴胺介导的柔性空间屏障促进纳米酶的多层组装以协同强化现场免疫分析
Xiaoxuan Li,Zhenguang Ji,Niu Feng et al.
Xiaoxuan Li et al.
The development of effective diagnostic strategies is crucial for ensuring the public health security. Here, we present a composite probe with exceptional peroxidase-like activity suitable for ultrasensitive and rapid immune-quantitative an...
Temperature-Modulated SERS Fusion Enables Accurate Diagnosis of Clinical Fungal Pathogens [0.03%]
温度调制SERS融合技术可实现临床真菌病原体的精准诊断
Lei Jin,Qiang Wang,Xiaojun Cai et al.
Lei Jin et al.
The rapid and accurate identification of fungal pathogens remains a significant challenge in clinical microbiology. Surface-enhanced Raman spectroscopy (SERS) shows great potential for fungal diagnostics; however, achieving fast, convenient...
Insight into the G-Quadruplex Topologies: A Rapid Separation and Identification Method of Multiple Coexisting Conformations by Capillary Zone Electrophoresis [0.03%]
一种快速分离和鉴定共存的G-四链体构象的方法：毛细管区带电泳研究G-四链体拓扑结构的新见解
Yi-Xuan Gu,Xu-Yang Shen,Yu-Nan Chen et al.
Yi-Xuan Gu et al.
G-quadruplexes (G4s) are noncanonical secondary structures, which have been demonstrated to be prevalent at gene regulatory regions in various genomes. In solution, G4s derived from single-stranded oligonucleotides can adopt different topol...
Flexible and Reusable Biochip Platform for miRNA Quantification via Dual-Signal Amplification on 3D Au Substrate [0.03%]
基于三维金基底双重信号扩增的用于miRNA定量的柔性可重复使用的芯片平台
Jieon Lee,Hee-Kyung Na,Woo-Keun Kim
Jieon Lee
Micro ribonucleic acids (miRNAs) are small, noncoding RNA molecules that are pivotal regulators of gene expression and have emerged as critical biomarkers for various diseases and therapeutic targets. Among these, circulating miRNAs in the ...