Haozhi Song,Zhayila Reheman,Ying Fang et al.
Haozhi Song et al.
Cellular targets can be imaged using biosensors composed of RNA. However, current RNA-based sensors encounter several challenges, including low brightness, poor photostability, slow response kinetics, and green background noise. In addition...
SMART: An Approach for Accurate Formula Assignment in Spatially Resolved Metabolomics [0.03%]
基于空间代谢组学的精确分子式指派方法SMART
Yinghao Cao,Shengxi Li,Zhaoyi Liu et al.
Yinghao Cao et al.
Spatially resolved metabolomics plays a critical role in unraveling tissue-specific metabolic complexities. Despite its significance, this profound technology generates thousands of features, yet accurate annotation significantly lags behin...
Deep Mining of Novel Acylated Polyamines by Integrated Prior-Knowledge-Guided Prediction and Chemical Isotope Labeling-Based Metabolomics [0.03%]
基于整合的先验知识指导预测和化学同位素标签代谢组学深度挖掘新型酰化多胺
Yiran Zhang,Qinwen Xiao,Xinyao Zhou et al.
Yiran Zhang et al.
Acylated polyamines (acyl-PAs) are gaining significant attention due to their involvement in various diseases. However, their annotation and quantification remain challenging, as robust analytical methods are lacking, with only a few acyl-P...
Monitoring the Chemical Transformation and Detoxification Process of Aconitum Herbs during Processing by Maximum-Quantum Filter NMR Spectroscopy [0.03%]
基于最大量子滤波NMR技术监测附子炮制过程中化学变化及减毒增效过程
Haipeng Jiang,Wenjing Zhong,Danlei Feng et al.
Haipeng Jiang et al.
Processing is an ancient technique to make Traditional Chinese Medicine (TCM) more effective or less toxic; however, the traditional process control for processing is empirical, and it is highly desired to monitor chemical markers that are ...
Two-Photon FLIM Imaging of Mitochondrial Microenvironment in Apoptosis, Ferroptosis, and Fatty Liver Disease Models Using a Carbazole-Pyridinium Probe [0.03%]
用于凋亡、铁死亡和脂肪肝疾病模型的线粒体微环境的双光子FLIM成像技术研究
Tong Zhu,Shang Wan,Hong Wang et al.
Tong Zhu et al.
Mitochondria are dynamic organelles whose microenvironmental state is tightly linked to cell death pathways and metabolic disease progression. However, directly visualizing mitochondrial microenvironment dynamics (e.g., viscosity changes) i...
Robust Automated Separation of Circulating Tumor Cells and Cancer-Associated Fibroblasts for Enhanced Liquid Biopsy in Breast Cancer [0.03%]
耐药性乳腺癌外渗细胞与肿瘤相关成纤维细胞的自动化分离以增强液体活检技术
Hyeong Jung Woo,Paul N Rademacher,Hyun Young Shin et al.
Hyeong Jung Woo et al.
Circulating tumor cells (CTCs) are a key biomarker in cancer diagnostics, offering critical insights into metastasis and treatment responses. Although several automated CTC isolation systems have been developed, a thorough comparison of the...
Nanozyme-Enabled Multimodal Sensing: Visual and Rapid Profiling of Extracellular Vesicles [0.03%]
纳米酶驱动的多模态检测：细胞外囊泡的可视化和快速剖析
Xiao Wang,Jing-Yuan Ma,Xiaoli Wei et al.
Xiao Wang et al.
CD20, a transmembrane protein on the surface of lymphoma extracellular vesicles (EVs), is highly expressed and serves as an effective marker for monitoring lymphoma subtypes and evaluating the efficacy of antibody therapy. Therefore, there ...
Time-Resolved Quantification of Single-Cell Extracellular Vesicles Secretion for Drug Resistance Evaluation [0.03%]
单细胞外泌体分泌的时间分辨量化以评估药物抗性问题
Haimeng Pei,Haibin Si,Dexin Du et al.
Haimeng Pei et al.
Extracellular vesicles (EVs) play a crucial role in mediating cell-cell communication and shaping cellular functions. However, the relationship between EVs secretion and cellular drug sensitivity, as well as the role of EVs in drug resistan...
Metal/Insulator/Metal Sandwich Structure Improves Background Potential Stability of Galvanic Redox Potentiometry [0.03%]
金属/绝缘体/金属夹心结构可提高伏安氧化还原电位法的背景电位稳定性
Siliang Zhang,Wenjie Ma,Yanyan Zhang et al.
Siliang Zhang et al.
Galvanic redox potentiometry (GRP) has been used in various research fields, particularly for in vivo neurotransmitter detection. However, background potential fluctuations significantly limit its application for detecting basal neurotransm...
Coupling Supercritical Fluid Chromatography with Internal Standard Corrected Quantitative NMR for Rapid and Accurate Purity Assessment [0.03%]
快速准确的纯度评估耦合超临界流体色谱与内标校正的定量核磁共振法
Huan Yao,Ting Huang,Zhifang Li et al.
Huan Yao et al.
Purity assessment is critical for the quality control of active pharmaceutical ingredients (APIs). However, there is a lack of rapid and accurate purity assessment methods that operate independently of target-specific certified reference ma...