EXAGO: An Argonaute-Based Primer-Free Exponential Amplification Strategy for Ultrasensitive Zero-Background Detection of Point Mutation [0.03%]
EXAGO：一种基于Argonaute的无引物指数扩增策略，用于超灵敏的零背景点突变检测
Lingyi Wu,Weijie Chen,Ru Huang et al.
Lingyi Wu et al.
Ultrasensitive gene detection is crucial for precise molecular diagnostics. Conventional nucleic acid amplification methods frequently encounter nonspecific amplification triggered by exogenous primers, which limits their utility in ultrase...
Integrated Single-Cell Array-Electrochemical Platform for Label-Free, Nondestructive Longitudinal Tracking of Osteogenic Differentiation [0.03%]
一种用于无标记、非破坏性纵向追踪成骨分化的单细胞阵列电化学平台
Yuanyuan Zhang,Jing Chen,Meihong Peng et al.
Yuanyuan Zhang et al.
Longitudinal, label-free tracking of stem cell differentiation is hindered by destructive end point assays and variability from cell migration and uncontrolled contacts. Here, we introduce an integrated single-cell array-electrochemical pla...
Suppression Probe Enrichment for Highly Sensitive and Multiplexed Detection of RAS Mutations in Colorectal Cancer [0.03%]
用于检测结直肠癌中RAS突变的高度灵敏且多重化的抑制探针富集法
Xinglei Su,Yunpei Si,Wenle Huang et al.
Xinglei Su et al.
Although RAS mutations are concentrated in specific hotspots, their clinical detection remains reliant on next-generation sequencing (NGS), which is hindered by high costs, long turnaround times, and substantial sample requirements. Herein,...
Rapid and Accurate Quantification of Viable Bacteria in Reproducibly Generated Bioaerosols with a Certified Microbial Reference Material [0.03%]
一种快速准确量化可重复生成生物气溶胶中活细菌的方法及其认证微生物参考物质
Meng Wang,Hanyue Ding,Zhenyan Zhang et al.
Meng Wang et al.
Bioaerosols play a pivotal role in the transmission of respiratory diseases, rendering bioprotective products essential for mitigating bioaerosol-mediated disease spread. However, current limitations in reproducible bioaerosol generation an...
Single-Cell Electrochemical Mass Spectrometry for Complementary Analysis of Cellular Secretions Induced by Mechanical Stimulation [0.03%]
单细胞电化学质谱联用技术全面解析机械刺激诱发的细胞分泌物
Caiying Zhang,Ting Li,Jiamei Lin et al.
Caiying Zhang et al.
Cellular mechanotransduction is an important physiological process. The cellular secretions induced by mechanical stimulation not only reflect the intricate intracellular mechanotransduction processes but also reveal the intercellular commu...
Heather M Felmy,Nikolas T C Boily,Adan Schafer Medina et al.
Heather M Felmy et al.
The ability to run chemical processing more efficiently and cost effectively is a need that spans critical materials recovery and legacy nuclear waste cleanup. Sensors integrated to provide online monitoring are essential to addressing this...
Quantitative 14N NMR with Monte Carlo Uncertainty Analysis of Nitrate/Nitrite in Alkaline Nuclear Waste [0.03%]
碱性核废水中硝酸盐/亚硝酸盐的定量14NNMR及蒙特卡罗不确定度分析
Trent R Graham,Jacob G Morton,Ashley R Kennedy et al.
Trent R Graham et al.
Quantification of nitrate and nitrite concentrations is important for the management of radioactive tank waste chemistry, as these species influence solubility and corrosion. However, existing analytical methods are potentially hindered by ...
Microwave-Assisted Synthesis of PNA@AuNRs with Temperature Gating for Precise Amplification of Nucleic Acids [0.03%]
一种基于温度控制的PNA@金纳米棒复合材料的微波合成及用于核酸精准扩增的新机制研究
Yirui Li,Zhipeng Wang,Xiaodong Sun et al.
Yirui Li et al.
Nucleic acid amplification tests are widely used for molecular diagnostics. However, primer-dimer formation and nonspecific amplification may compromise analytical accuracy and increase the risk of false-positive results. Here, temperature-...
Label-Free, AI-Driven Evanescent Microscopy Decodes Single-Cell Membrane Protein Binding Kinetics and Adhesion Biomechanics [0.03%]
无标签、基于AI的倏逝波显微镜解码单细胞膜蛋白结合动力学及黏附生物力学
Caixin Huang,Jingbo Zhang,Zhaoyang Liu et al.
Caixin Huang et al.
Quantifying ligand interactions with membrane proteins in their native cellular environment is crucial for understanding cellular processes and drug screening, yet it remains a challenging task. Here, we introduce an artificial intelligence...
A Proximal-Guaranteed Dual-Marker Electrochemiluminescence System for Accurate Identification of Colorectal Cancer Cell Subpopulations [0.03%]
一种用于准确识别结直肠癌细胞亚群的近端保证双重标志电化学发光系统
Yunfan Zhou,Xue Wei,Ling Hai et al.
Yunfan Zhou et al.
Accurate identification of cancer cell subgroups is a crucial foundation for targeted therapy and prognosis prediction of cancer. However, existing detection systems lack synergy between spatial proximity assurance and signal resolution, po...