Noble Metal-Metal Oxide Nanohybrids as a High-Performance LDI-MS Matrix for Machine Learning-Driven Metabolic Diagnosis of Esophageal Cancer [0.03%]
贵金属-金属氧化物纳米杂化材料作为基于机器学习的食管癌代谢诊断高灵敏质谱分析基质
Chaoqi Wang,Mengdan Shi,Peilin Peng et al.
Chaoqi Wang et al.
Esophageal cancer represents a global health challenge with a notably high incidence and poor prognosis, necessitating the development of rapid, noninvasive diagnostic methodologies. In this study, we present a high-throughput metabolomics ...
Top-Down versus Bottom-Up Proteomics in Highly Sensitive LC-MS-Based Profiling of Limited Samples [0.03%]
有限样本的高灵敏度LC-MS分析中的自上而下与自下而上蛋白质组学
Yunfan Gao,Michal Greguš,James C Kostas et al.
Yunfan Gao et al.
The advantages of top-down proteomics (TDP) in the characterization of proteoforms, resulting from genetic variations, alternative splicing, and post-translational modifications (PTMs), have been well documented. However, TDP applications o...
Water Activation Enables Low-Triggering-Potential [Ru(bpy)3]2+-Tripropylamine Electrochemiluminescence [0.03%]
水活化实现低触发电位的[Ru(bpy)₃]²⁺-三丙胺电致化学发光
Juan He,Yan Zhou,Siting Wu et al.
Juan He et al.
Low-triggering-potential electrochemiluminescence (ECL) systems based on tris(2,2'-bipyridyl) ruthenium(II)/tripropylamine ([Ru(bpy)3]2+/TPrA) are essential for minimizing electrochemical interference and enabling highly sensitive biodetect...
UNMR-Net: A Unified Deep Reconstruction Network for Multitask Compressed Sensing NMR Spectroscopy [0.03%]
一种用于多任务压缩感知NMR光谱的统一深度重建网络（UNMR-Net）
Haolin Zhan,Zhixin Ye,Chaojie Xing et al.
Haolin Zhan et al.
NMR spectroscopy has become an indispensable tool in chemistry and life sciences, yet its high resolution is generally constrained by the cost of increased acquisition times. Recently, sparse data sampling combined by deep learning (DL) rec...
Evaluating DIA LiP-MS Analysis Workflows with a Hybrid LiP Proteome Benchmark [0.03%]
基于混合型LiP蛋白质组基准评估DIA LiP-MS分析工作流
Shanshan Li,Shijia Yuan,Huiting Luo et al.
Shanshan Li et al.
Emerging as a powerful structural proteomics approach, limited proteolysis mass spectrometry (LiP-MS) has been widely employed to interrogate proteome-wide protein structural alterations, identify drug targets and drug-binding pockets, and ...
Establishing Orbitrap-Based Methods for Multi-Element Compound-Specific Isotope Analysis of Per- and Polyfluoroalkyl Substances [0.03%]
基于轨道阱的方法建立用于全氟和多氟烷基物质的多元素化合物特异性同位素分析
Juyoung Park,Linnea J Heraty,Neil C Sturchio et al.
Juyoung Park et al.
Per- and polyfluoroalkyl substances (PFAS) are globally distributed environmental contaminants whose persistence and toxicity necessitate advanced source identification strategies. Stable isotope analysis serves as a powerful forensic tool,...
Thermally Unlocked One-Pot RPA-CRISPR Cas12b Assay Integrated with the Centrifugal Microfluidic Chip for Multiplex Detection of Porcine Viruses [0.03%]
基于离心微流控芯片的热激释放型RPA-CRISPR Cas12b联检猪病毒的一管式检测体系
Mingyan Li,Di Huang,Chutian Xu et al.
Mingyan Li et al.
Highly contagious porcine viruses, represented by African swine fever virus (ASFV), porcine reproductive and respiratory syndrome virus (PRRSV), and pseudorabies virus (PRV), inflict severe economic losses on the swine industry and pose sig...
Membrane Fusion-Assisted Laser Desorption/Ionization Mass Spectrometry for In Situ Extracellular Vesicle Metabolic Fingerprinting [0.03%]
膜融合辅助激光解吸电离质谱技术用于细胞外囊泡代谢指纹鉴定
Qian Shi,Yuerong Tang,Haonan Yang et al.
Qian Shi et al.
Metabolites packaged within extracellular vesicles (EVs) are increasingly recognized for their role in intercellular communication and metabolic regulation. However, their comprehensive characterization remains challenging due to the low ab...
Ligand-Driven Supramolecular Assembly of Gold Nanoclusters with Ultralong Lifetime and Large Stokes Shift for High-Efficiency Electrochemiluminescence [0.03%]
高效电化学发光的长寿命大斯托克斯位移金纳米团簇超分子组装体及其驱动配体研究
Mingyang Xia,Hongyan Liu,Congrui Zhang et al.
Mingyang Xia et al.
Achieving high-efficiency electrochemiluminescence (ECL) with an ultralong lifetime and a large Stokes shift in a single emitter is highly desirable yet challenging. Herein, we report the supramolecular assembly of gold nanoclusters (Au NCs...
A One-Drop Quantitative Concentration Pipette Using Micro- to Centimeter-Scale Fluidics [0.03%]
一种使用微米至厘米级流体学的单滴定量浓度移液管
Ruying Wang,Suyang Liu,Xuan Yan et al.
Ruying Wang et al.
The analytical process is typically divided into three stages: pretreatment, assay, and detection. In the assay and detection stages, some analytical platforms require very small sample volumes. Small sample volumes can increase reaction ra...