sEV-ICP-TOF-MS for Multiparametric Single-EV Analysis via Nanoparticle-Conjugated Antibodies and Isotope Ratio Filtering [0.03%]
基于纳米颗粒偶联抗体和同位素比率过滤的单个EV多参数分析(SEV-ICP-TOF-MS)方法
Minseop Kim,Seung Min Ha,Eunyong Ha et al.
Minseop Kim et al.
Extracellular vesicles (EVs) play a vital role in cell-to-cell communication and hold great promise for diagnostics and therapy. However, their small size, heterogeneity, and interference with other nanoparticles make them difficult to dete...
Probing the Growth Kinetics of Bioinspired Phenolic Nanocoating via Evanescent Wave Excited by Fiber-Optic Modal Interferometry [0.03%]
基于光纤模式干涉的倏逝波激发技术用于生物启发型酚醛纳米涂层动力学研究
Xin Xin,Junjun Wu,Qiang Ling et al.
Xin Xin et al.
Bioinspired polydopamine (PDA) nanocoating poses one important measure to precisely customize functionalized surfaces, while its dynamic growth characteristics are still not fully understood. Herein, we develop a high-sensitivity and rapid-...
Amplification by Polysaccharides for the Electrochemical Detection of Trypsin Activity [0.03%]
基于糖胺聚糖放大作用的胰蛋白酶电化学检测方法研究
Junpeng Lv,Jingjing Xu,Yiting Chen et al.
Junpeng Lv et al.
Although polysaccharides are ubiquitous in nature, biodegradable, and cheap, there is no report illustrating their use as functional materials for signal amplification in the biosensing of enzyme activities. Herein, we report the developmen...
A Structure-Guided Bifunctional Aptamer Mitigates Pseudomonas aeruginosa Virulence and Biofilms by Disrupting las and rhl Quorum Sensing Systems [0.03%]
一种结构导向的双功能核酸适配体通过破坏PQS、las和rhl淬火系统来抑制绿脓杆菌的毒力和生物被膜形成
Kuaile Wu,Yalin Mo,Mengxia Duan et al.
Kuaile Wu et al.
Quorum sensing (QS) is a microbial communication system that mediates the virulence factor synthesis and biofilm formation through signaling molecules. QS-targeted antivirulence strategies have emerged as a new approach to avoiding antibiot...
Micelle-Confined Co-N Coordination Enables Size-Tailored Nanoemitters for High-Performance ECL Biosensing [0.03%]
micelle限定的Co-N配位用于尺寸可调的纳米发射体以实现高性能的化学发光生物传感
Yu-Xuan Dai,Yi-Xuan Li,Jérome Chauvin et al.
Yu-Xuan Dai et al.
Electrochemiluminescence (ECL) is particularly suited for ultrasensitive biomarker detection due to its low background signal and tunable electrochemical control. Herein, we report a micelle-confined coordination engineering strategy for th...
Aptamer-Based Molecule-Responsive SERS System for Highly Efficient Detection of Cocaine [0.03%]
一种高效的检测可卡因的适配子分子响应型拉曼系统
Tingting Xiao,Weiqiang Wang,Lingyi Zhao et al.
Tingting Xiao et al.
Illicit drug abuse has brought big challenges to public health, because it has caused severe damage to the human central nervous system. The development of a rapid, quantified, and on-site detection of illicit drugs is of key importance for...
Artificial Peak Laser Absorption Spectroscopy: Simultaneous Quantification of Temperature and Concentration with Extended Dynamic Range [0.03%]
人工峰激光吸收光谱法：扩展量程的温度和浓度同时定量测量方法
Jiachen Sun,Jiankun Shao,Dezhi Zheng
Jiachen Sun
This study presents an innovative calibration-free artificial peak-laser absorption spectroscopy (AP-LAS) technique that fundamentally overcomes the inherent dynamic range-sensitivity trade-off and eliminates reliance on specific absorption...
Integrating Multiple MS Techniques for in-Depth Antibody Glycosylation Analysis: Revealing Glycosylation-Dependent Structural and Functional Properties [0.03%]
多种MS技术的整合用于深入的抗体糖基化分析：揭示糖基化依赖性的结构和功能属性
Yuye Zhou,Sophia Schedin Weiss,Congrui Tan et al.
Yuye Zhou et al.
Glycosylation plays a critical role in modulating protein structure, stability, and binding properties, yet comprehensive tools to systematically characterize these effects are scarce. Here, we integrated multiple mass spectrometry (MS) tec...
Investigating Interfacial Interactions between the Adjuvant and Antigens in Pneumococcal Conjugate Vaccine In Situ at the Molecular Level [0.03%]
基于分子水平的肺炎球菌结合疫苗中佐剂与抗原相互作用的研究
Yuchen Wu,Zahra Gandhi,Daniel Rossi et al.
Yuchen Wu et al.
Despite recent advances in prevention and treatment, pneumococcal disease is still a leading cause of serious illness and death among young children and elderly adults. Novel multivalent pneumococcal conjugate vaccines (PCVs) are under deve...
Quantitative Control of Zn2+ Photorelease: A Step toward Decoding Mechanisms of Subsecond Metal Signaling in the Brain [0.03%]
定量控制Zn²⁺光释放：迈向解码大脑亚秒金属信号机制的第一步
Sayuri Niyangoda,Jodie Kearney,Piyanka Hettiarachchi et al.
Sayuri Niyangoda et al.
Free ionic zinc (Zn2+) exerts control over many aspects of neuronal function after its exocytotic release from vesicles by associating with conserved binding sites on regulatory proteins, including the dopamine transporter. While the use of...