Proximity Singlet-Oxygen-Activation and Luminescence-Controllable Chemiluminescent Assay for Fast Ultrasensitive Detection of Pathogenic Bacteria [0.03%]
一种快速超灵敏检测致病菌的近体单线态氧激活和可控发光的化学发光法
Daian Chen,Ling Xie,Ruiying Peng et al.
Daian Chen et al.
In this study, a novel proximity singlet oxygen (1O2)-activation and luminescence-controllable chemiluminescent assay (PALCA) was developed for ultrasensitively detecting pathogenic bacteria. The PALCA system comprised an antimicrobial pept...
Rigid Restriction-Enabled Ultrabright Luminescence through Feather-like Metal-AIEgens Frameworks and Coupling the Collaborative Interfacial Recognition Mechanism for Pathogen Diagnosis [0.03%]
基于羽毛状金属AIEgens框架的rigid restriction-enabled超亮发光及用于病原体诊断的协同界面识别机制耦合研究
Zhaowen Cui,Chenjie Nie,Yuechun Li et al.
Zhaowen Cui et al.
The pursuit of high-performance immunochromatographic assays (ICAs) is hindered by inadequate signal brightness and the lack of understanding of the nanobiointerfacial recognition mechanism. Herein, a featherlike zinc-based metal-AIEgen fra...
Antibody-Free and Rapid Profiling of Transcription Factor Binding Sites by Aptamer-Guided Chromatin Cleavage and Sequencing (AptaChC-Seq) [0.03%]
基于适配体引导的染色质切割与测序的无抗体快速转录因子结合位点图谱绘制技术(AptaChC-Seq)
Xucong Teng,Qiushuang Zhang,Yuncong Wu et al.
Xucong Teng et al.
Mapping transcription factor (TF) binding sites is crucial for understanding the regulatory mechanisms by which TFs and associated proteins control biological processes. Existing methods rely on antibodies as protein-binding reagents, makin...
Efficient Discovery of Immunoglobulin Genes for Detecting Organophosphorus Pesticides Using a Reverse Virtual Screening Pipeline [0.03%]
利用反向虚拟筛选管道高效发现用于检测有机磷农药的免疫球蛋白基因
Rubing Zou,Yuanhao Guo,Zhenyu Wang et al.
Rubing Zou et al.
Hapten-specific recombinant antibodies (rAbs) are increasingly crucial elements in bioanalytical chemistry for tracing small molecules, but identifying anti-hapten immunoglobulin genes remains challenging. Here, we established a reverse vir...
SPIN: Inkjet-Driven Nanowell Workflow for Scalable and Sensitive Single-Cell Proteomics [0.03%]
SPIN：一种基于喷墨打印的单细胞蛋白质组学纳米井工作流程
Eric Cheng,Shuxin Chi,Huan Zhong et al.
Eric Cheng et al.
Single-cell proteomics is emerging as a powerful approach to resolve cellular heterogeneity, yet sample processing remains challenging due to limited input material and the absence of protein amplification. We present a protocol centered on...
AI-Integrated Conformal Cellulose Nanofiber-Reinforced Hydrogel Patch for Dual-Ratiometric Fluorescence Monitoring of Pyrimethanil Residues [0.03%]
一种用于嘧霉胺残留双重比度荧光检测的集成人工智能对形纤维增强水凝胶补丁
Li Li,Rumin Fu,Weihao Li et al.
Li Li et al.
Real-time and on-site monitoring of pesticide residues remains challenging due to the lack of plant-interfaced, actively enriching, and nondestructive analytical devices. Herein, we present an intelligent, plant-conformal cellulose nanofibe...
Precisely Identifying Growth Phases of Living Bacteria using Open-Set Deep Learning-Driven Single-Cell Raman Spectroscopy [0.03%]
基于开集深度学习的单细胞拉曼光谱在活细菌生长阶段精确诊断中的应用研究
Yufeng Yuan,Yifan Sun,Fusheng Du et al.
Yufeng Yuan et al.
Owing to population cellular heterogeneity, it is still challenging to identify and determine the growth phases of spore-forming bacterial cells from a complex culture environment with a single-cell resolution. Knowledge of the physiologica...
POx-Lipids as an Alternative to PEG-Lipids? Multimethod Assessment of Chemistry and Structure [0.03%]
POx-脂质作为PEG-脂质的替代品吗？化学和结构的多方法评估
Ekaterina Tsarenko,Ilya Anufriev,Caroline T Holick et al.
Ekaterina Tsarenko et al.
The characterization of polymer-lipid conjugates is essential for their successful application in pharmaceutical formulations, particularly in lipid nanoparticles (LNPs). While poly(ethylene glycol) (PEG) lipids are widely used in LNPs, gro...
Fluorogenic Ligand Enables Identification of NSD3-Overexpressed Tumors by Targeting the PWWP1 Domain of NSD3 [0.03%]
荧光配体通过靶向NSD3的PWWP1结构域来识别NSD3高表达肿瘤
Ying Li,Songjun Shao,Qian Lei et al.
Ying Li et al.
Nuclear receptor-binding SET domain-containing 3 (NSD3) is a histone-modifying enzyme that is responsible for the dimethylation of lysine 36 of histone 3 (H3K36), a mark associated with active gene transcription. The human NSD3 is frequentl...
Integrated Enzyme-Powered DNA-Gold Nanomachine for In Vivo Amplified mRNA Photoacoustic Imaging and Enhanced Tumor Photothermal Therapy [0.03%]
一种集成酶驱动的DNA-金纳米机器用于放大mRNA光声成像和增强肿瘤光热治疗
Mingzhu Fan,Huiping Liang,Yifang He et al.
Mingzhu Fan et al.
Developing activatable nanotheranostics that can improve diagnostic and therapeutic performances and reduce side effects is highly attractive for precision cancer medicine but remains a daunting challenge. Herein, we develop an integrated e...